Organization and morphology of thalamocortical neurons of mouse ventral lateral thalamus

Tlamsa, Aileen P.; Brumberg, Joshua C.

doi:10.3109/08990221003646736

Cited by 43 publications

(21 citation statements)

References 29 publications

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“…5B-D). The morphology of these cells resembled that of previously described thalamocortical sensory relay neurons (Tlamsa AP and JC Brumberg 2010). This connectivity pattern is hence similar to what has previously been described for other CST neurons (Abraira VE and DD Ginty 2013;Constantinople CM and RM Bruno 2013;McMahon et al 2013).…”

Section: S1-cst Neurons Receive Input From the Somatosensory Circuitsupporting

confidence: 88%

In-depth characterization of layer 5 output neurons of the primary somatosensory cortex innervating the mouse dorsal spinal cord

Frezel

Mateos

Platonova

et al. 2020

Preprint

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Neuronal circuits of the spinal dorsal horn integrate sensory information from the periphery with inhibitory and facilitating input from higher CNS areas. Most previous work focused on descending projections originating from the hindbrain. Less is known about the organisation of inputs descending from the cerebral cortex. Here, we identified cholecystokinin (CCK) positive layer 5 pyramidal neurons of the primary somatosensory cortex (S1-CST neurons) as a major source of descending input to the spinal dorsal horn. We combined intersectional genetics and virus-mediated gene transfer to characterize CCK + S1-CST neurons and to define their presynaptic input and postsynaptic target neurons. We found that S1-CST neurons constitute a heterogeneous population that can be subdivided into distinct molecular subgroups. Rabies-based retrograde tracing revealed monosynaptic input from layer 2/3 pyramidal neurons, from parvalbumin (PV) and neuropeptide Y (NPY) positive cortical interneurons, and from thalamic relay neurons in the ventral posterolateral nucleus. WGA-based anterograde tracing identified postsynaptic target neurons in dorsal horn laminae III and IV. About 60% of these neurons were inhibitory and about 60% of all spinal target neurons expressed the transcription factor c-Maf. The heterogeneous nature of both S1-CST neurons and their spinal targets suggest sophisticated roles in the fine-tuning of sensory processing.Research University (PSL), Paris 75005, France. E. Platonova has been supported through the Technology Platform commission of the University of Zürich. We want to thank C. Kaiser for the technical support and F. Voigt and F. Helmchen for the support with the MesoSPIM.

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Section: S1-cst Neurons Receive Input From the Somatosensory Circuitsupporting

confidence: 88%

In-depth characterization of layer 5 output neurons of the primary somatosensory cortex innervating the mouse dorsal spinal cord

Frezel

Mateos

Platonova

et al. 2020

Preprint

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“…Somatosensory information also reaches M1 via higher-order sensory thalamus, including the medial subdivision of posterior nucleus (POm) (Deschenes et al, 1998; Ohno et al, 2011) and ventrolateral thalamus (VL) (Asanuma et al, 1980; Asanuma et al, 1979; Kuramoto et al, 2009; Nambu et al, 1988; Strick and Sterling, 1974). Motor thalamus consists of several nuclei, including the anteromedial (AM), ventral anterior (VA) and ventrolateral (VL) nuclei (Kuramoto et al, 2009; Tlamsa and Brumberg, 2010). Frontal cortical areas also project to M1 (Dum and Strick, 2002; Muakkassa and Strick, 1979; Reep et al, 1990; Rouiller et al, 1993) and may activate M1 during voluntary movements, in contrast to circuits involved in sensory-guided actions (Haggard, 2008; Smith et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Organization of Cortical and Thalamic Input to Pyramidal Neurons in Mouse Motor Cortex

et al. 2013

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Determining how long-range synaptic inputs engage pyramidal neurons in primary motor cortex (M1) is important for understanding circuit mechanisms involved in regulating movement. We used channelrhodopsin-2 (ChR2)-assisted circuit mapping to characterize the long-range excitatory synaptic connections made by multiple cortical and thalamic areas onto pyramidal neurons in mouse vibrissal motor cortex (vM1). Each projection innervated vM1 pyramidal neurons with a unique laminar profile. Collectively, the profiles for different sources of input partially overlapped and spanned all cortical layers. Specifically, orbital cortex (OC) inputs primarily targeted neurons in L6. Secondary motor cortex (M2) inputs excited neurons mainly in L5B, including pyramidal tract (PT-type) neurons. In contrast, thalamocortical inputs from anterior motor-related thalamic regions, including VA/VL, targeted neurons in L2/3 through L5B, but avoided L6. Inputs from posterior sensory-related thalamic areas, including POm, targeted neurons only in the upper layers (L2/3 and L5A), similar to inputs from somatosensory (barrel) cortex. Our results show that long-range excitatory inputs target vM1 pyramidal neurons in a layer-specific manner. Inputs from sensory-related cortical and thalamic areas preferentially target the upper-layer pyramidal neurons in vM1. In contrast, inputs from OC and M2, areas associated with volitional and cognitive aspects of movements, bypass local circuitry and have direct monosynaptic access to neurons projecting to brainstem and thalamus.

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“…Among the nuclei of the ventral thalamic group, VL sends a good number of projections to S1BF. It is well established that VL provides somatotopic motor information to the motor cortex (Strick, 1973;Schmahmann, 2003;Tlamsa and Brumberg, 2010), however, there is a small number of neurons in VL that projects to S1 (Donoghue et al, 1979;Spreafico et al, 1981). As we show here, this projection could target specifically S1BF.…”

Section: Top-down and Multisensory Thalamocortical Connections To S1mentioning

confidence: 50%

Afferent connections of the primary somatosensory cortex of the mouse for contextual and multisensory processing

Massé

Blanchet-Godbout

Bronchti

et al. 2019

Preprint

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Sensory information is conveyed from peripheral receptors through specific thalamic relays to primary areas of the cerebral cortex. Information is then routed to specialized areas for the treatment of specific aspects of the sensory signals and to multisensory associative areas. Information processing in primary sensory cortices is influenced by contextual information from top-down projections of multiple cortical motor and associative areas as well as areas of other sensory modalities and higher order thalamic nuclei. The primary sensory cortices are thus located at the interface of the ascending and descending pathways. The theory of predictive coding implies that the primary areas are the site of comparison between the sensory information expected as a function of the context and the sensory information that comes from the environment. To better understand the anatomical basis of this model of sensory systems we have charted the cortical and subcortical afferent inputs in the ipsilateral and contralateral hemispheres of the primary somatosensory cortex of adult C57Bl/6 mice. Iontophoretic injections of the b-fragment of cholera toxin were performed inside the mystacial caudal barrel field, more rostral barrel field and somatosensory cortex outside the barrel field to test the hypothesis that differences exist between these three parts and to compare their projections to the subnetworks built from the Mouse Connectome Project data. The laminar distribution of retrogradely labeled cell bodies was used to classify the projections as feedback, feedforward or lateral. Layer indices range between −1 and 1, indicating feedback and feedforward connections respectively. The primary somatosensory cortex and the barrel field have afferent connections with somatosensory areas, non-somatosensory primary sensory areas, multisensory, motor, associative, and neuromodulatory areas. The caudal part of the barrel field displays different and more abundant cortical and subcortical connections compared to the rest of the primary somatosensory cortex. Layer indices of cortical projections to the primary somatosensory cortex and the barrel field were mainly negative and very similar for ipsilateral and contralateral projections. These data demonstrate that the primary somatosensory cortex receives sensory and non-sensory information from cortical and subcortical sources.

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Organization and morphology of thalamocortical neurons of mouse ventral lateral thalamus

Cited by 43 publications

References 29 publications

In-depth characterization of layer 5 output neurons of the primary somatosensory cortex innervating the mouse dorsal spinal cord

In-depth characterization of layer 5 output neurons of the primary somatosensory cortex innervating the mouse dorsal spinal cord

Organization of Cortical and Thalamic Input to Pyramidal Neurons in Mouse Motor Cortex

Afferent connections of the primary somatosensory cortex of the mouse for contextual and multisensory processing

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