Organization and transcription of the gluconate operon, gnt, of Bacillus subtilis.

Crystallographic study of coenzyme, coenzyme analogue and substrate binding in 6-phosphogluconate dehydrogenase: implications for NADP specificity and the enzyme mechanism

“…The enzyme is dimeric and NADP-dependent for almost all species [1]. Seven complete primary sequences have been determined (for sheep [2]; Trypanosoma brucei [3]; Escherichia coli [4]; Salmonella typhimurium [5]; Synechococcus [6]; Bacillus subtilis [7]; Drosophila melanogaster [8]) and two further partial sequences (pig [9,10]; mouse: S Hoffmann, personal communication). The subunit contains between 468 (E. coli) and 482 residues (sheep), 88 of which are conserved in all sequences reported so far.…”

Deficiency of GntR Family Regulator MSMEG_5174 Promotes Mycobacterium smegmatis Resistance to Aminoglycosides via Manipulating Purine Metabolism

“…The GntR family transcriptional regulators, originally identified in Bacillus subtilis ( B. subtilis ), are named after a gluconate operon repressor ( Fujita et al, 1986 ). These family regulators are widespread in bacteria and possess a conserved N-terminal DNA-binding domain and a C-terminal domain with the variable in length and structure ( Suvorova et al, 2015 ).…”

Engineering and characterization of probiotics to tackle fructose-induced metabolic syndrome

“…Besides FabA and FabB, FadR is involved in the regulation of a host of fatty acid degradation genes in E. coli such as FadD, FadE and FadH [133]. FadR is a member of the GntR family of transcription factors that operate as dimers comprising of a ligand-binding domain at the Cterminus and a DNA binding helix-turn-helix domain at the N-terminus [134,135]. In the absence of fatty acids, FadR binds to its cognate operator site within the promoter region of the fatty acid degradation genes, and thereby acting as steric hindrance to transcription by preventing RNA polymerase (RNAP) from coupling with the promoter.…”