Organization of a 117-kb circular mitochondrial chromosome in IR36 rice

Narayanan, K. K.; André, Charles P.; Yang, Jinshui; Walbot, Virginia

doi:10.1007/bf00351503

Cited by 20 publications

(5 citation statements)

References 28 publications

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“…When mtDNA is extracted by in-gel procedures and fractionated by pulsed-field gel electrophoresis (PFGE), the DNA that migrates in the gel is found in linear form at m50 to 150 kb in size and much longer linear molecules, but much (or most) remains at the origin of electrophoresis as an immobile wellbound form of mtDNA (Bendich, 1993;Narayanan et al, 1993;André and Walbot, 1995;Muise and Hauswirth, 1995). Because restriction fragment mapping predicts a circular form for the genome and its subgenomic fractions, the immobility of mtDNA has usually been ascribed to relaxed circular molecules trapped by agarose fibers, or the well-bound mtDNA is simply ignored.…”

Section: Introductionmentioning

confidence: 99%

Size and Structure of Replicating Mitochondrial DNA in Cultured Tobacco Cells

Oldenburg¹,

Bendich²

1996

The Plant Cell

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The BY-2 tobacco cell line was used to study the size and structure of replicating mitochondrial DNA (mtDNA). Approximately 70 to 90% of the newly synthesized mtDNA did not migrate during pulsed-field gel electrophoresis. Moving pictures of the fluorescently labeled molecules showed that most of the immobile well-bound DNA was in structures larger than the size of the BY-2 mitochondrial genome of -270 kb. Most of the structures appeared as complex forms with multiple DNA fibers. The sizes of the circular molecules that were also observed ranged continuously from -20 to 560 kb without prominent size classes. Pulse-chase and mung bean nuclease experiments showed that the well-bound DNA contained single-stranded regions and was converted to linear molecules of between 50 and 150 kb. MtDNA replication in plants may be initiated by recombination events that create branched structures of multigenomic concatemers that are then processed to 50-to 150-kb subgenomic fragments.

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Section: Introductionmentioning

confidence: 99%

Size and Structure of Replicating Mitochondrial DNA in Cultured Tobacco Cells

Oldenburg¹,

Bendich²

1996

The Plant Cell

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“…In the maize BMS cultivar, use of pulsed field gel electrophoresis allowed the mapping of a 120-kb chromosome that does not contain any large repeats, suggesting that it cannot be generated by recombination from a master circle and must be maintained autonomously (LEVY,ANDRE and WALBOT 199 1). The genome organization of multiple autonomous chromosomes has also been suggested in the development of a physical map of the rice mitochondrial genome (YAMATO et al 1992;NARAYANAN et al 1993). Therefore, the predicted configuration The proposed model of the genome in common bean is consistent with six genomic environments surrounding repeats R1 and R2.…”

Section: Discussionmentioning

confidence: 65%

“…Recent mapping data in petunia (FOLKERTS and HANSON 199 l), rice (YAMATO et NARAYANAN et al 1993) and maize (LEVY, ANDRE and WALBOT 1991) suggest that the structure of the plant mitochondrial genome in some species includes more than one autonomous molecule. The configuration of these molecules could not be predicted based on homologous recombinations; consequently, they could not be referred to as subgenomic molecules.…”

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confidence: 99%

“…The configuration of these molecules could not be predicted based on homologous recombinations; consequently, they could not be referred to as subgenomic molecules. These autonomous mitochondrial DNA molecules were designated as chromosomes (LEVY, ANDRE and WALBOT 1991;ANDRE and WALBOT 1992;NARAYANAN et al 1993). Rare recombination events across small repeats, or site-specific recombination, are considered a mechanism for the generation of these molecules (MANNA and BRENNICKE 1986; ANDRE, LEVY and WALBOT 1992).…”

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confidence: 99%

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Unusual mitochondrial genome organization in cytoplasmic male sterile common bean and the nature of cytoplasmic reversion to fertility.

Jańska¹,

Mackenzie²

1993

Genetics

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Spontaneous reversion to pollen fertility and fertility restoration by the nuclear gene Fr in cytoplasmic male sterile common bean (Phaseolus vulgaris L.) are associated with the loss of a large portion of the mitochondrial genome. To understand better the molecular events responsible for this DNA loss, we have constructed a physical map of the mitochondrial genome of a stable fertile revertant line, WPR-3, and the cytoplasmic male sterile line (CMS-Sprite) from which it was derived. This involved a cosmid clone walking strategy with comparative DNA gel blot hybridizations. Mapping data suggested that the simplest model for the structure of the CMS-Sprite genome consists of three autonomous chromosomes differing only in short, unique regions. The unique region contained on one of these chromosomes is the male sterility-associated 3-kb sequence designated pvs. Based on genomic environments surrounding repeated sequences, we predict that chromosomes can undergo intra- and intermolecular recombination. The mitochondrial genome of the revertant line appeared to contain only two of the three chromosomes; the region containing the pvs sequence was absent. Therefore, the process of spontaneous cytoplasmic reversion to fertility likely involves the disappearance of an entire mitochondrial chromosome. This model is supported by the fact that we detected no evidence of recombination, excision or deletion events within the revertant genome that could account for the loss of a large segment of mitochondrial DNA.

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“…Regardless of its genomic size or its restriction-endonuclease-generated genomic map (circular or linear), when fractionated by pulsed-field gel electrophoresis (PFGE) most plant mtDNA migrates as a smear zone corresponding to 50 to 200 kb linear DNA molecules. However, much of the mtDNA from all plants remains in the well at the origin of electrophoresis (André and Walbot, 1995;Backert et al, 1997;Bendich, 1996;Dai et al, 2005;Maleszka et al, 1991;Narayanan et al, 1993;Oldenburg and Bendich, 1996;Sakai et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Characterization of the Structure and DNA Complexity of Mung Bean Mitochondrial Nucleoids

Hsiao

Cheng

et al. 2011

Molecules and Cells

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Electron microscopic images of mitochondrial nucleoids isolated from mung bean seedlings revealed a relatively homogeneous population of particles, each consisting of a chromatin-like structure associated with a membrane component. Association of F-actin with mitochondrial nucleoids was also observed. The mitochondrial nucleoid structure identified in situ showed heterogeneous genomic organization. After pulsed-field gel electrophoresis (PFGE), a large proportion of the mitochondrial nucleoid DNA remained in the well, whereas the rest migrated as a 50-200 kb smear zone. This PFGE migration pattern was not affected by high salt, topoisomerase I or latrunculin B treatments; however, the mobility of a fraction of the fastmoving DNA decreased conspicuously following an in-gel ethidium-enhanced UV-irradiation treatment, suggesting that molecules with intricately compact structures were present in the 50-200 kb region. Approximately 70% of the mitochondrial nucleoid DNA molecules examined via electron microscopy were open circles, supercoils, complex forms, and linear molecules with interspersed sigmashaped structures and/or loops. Increased sensitivity of mtDNA to DNase I was found after mitochondrial nucleoids were pretreated with high salt. This result indicates that some loosely bound or peripheral DNA binding proteins protected the mtDNA from DNase I degradation.

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Organization of a 117-kb circular mitochondrial chromosome in IR36 rice

Cited by 20 publications

References 28 publications

Size and Structure of Replicating Mitochondrial DNA in Cultured Tobacco Cells

Size and Structure of Replicating Mitochondrial DNA in Cultured Tobacco Cells

Unusual mitochondrial genome organization in cytoplasmic male sterile common bean and the nature of cytoplasmic reversion to fertility.

Characterization of the Structure and DNA Complexity of Mung Bean Mitochondrial Nucleoids

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