Organization of cortical microtubules in differentiated cells

Zhou, Ping; Gao, Yang; Xie, Wei

doi:10.1002/jcp.31011

Cited by 3 publications

(3 citation statements)

References 48 publications

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“…This unique organization of microtubules in epithelial cells was initially discovered in the late 1980s using hook decoration methods and electron microscopy [15]. This arrangement of microtubules allows the sorting of specific membrane components via directing vesicle trafficking to distinct apical and basolateral regions of the plasma membrane [16].…”

Section: Distinct Orientation and Organization Of Microtubules In Epi...mentioning

confidence: 99%

“…There is no precise mechanism explaining how the non-centrosomal MTOC in apical regions is translocated to a centrosomal localization as epithelial cells transition to mesenchymal cells [13,16]. Decreases in microtubule nucleation, polymerization and stabilization along adherent junctions and apical regions are possible mechanisms that may lead to the displacement of non-centrosomal MTOC to the centrosome adjacent to the nucleus and Golgi structures [6].…”

Section: Reversion Of Mtoc From Non-centrosomal To Centrosomal Positi...mentioning

confidence: 99%

“…Par3 is present along the epithelial cell junction and is capable of recruiting dynein, which pulls on microtubules, and thus, can determine the centrosome position during planar cell polarity establishment. The amount of polymerized tubulin also contributes to the transition from an off-centered microtubule network in epithelial cells to a centered conformation in mesenchymal cells [16].…”

Section: Reversion Of Mtoc From Non-centrosomal To Centrosomal Positi...mentioning

confidence: 99%

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Regulation of Cell Adhesion and Migration via Microtubule Cytoskeleton Organization, Cell Polarity, and Phosphoinositide Signaling

Thapa,

Wen,

Cryns

et al. 2023

Biomolecules

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The capacity for cancer cells to metastasize to distant organs depends on their ability to execute the carefully choreographed processes of cell adhesion and migration. As most human cancers are of epithelial origin (carcinoma), the transcriptional downregulation of adherent/tight junction proteins (e.g., E-cadherin, Claudin and Occludin) with the concomitant gain of adhesive and migratory phenotypes has been extensively studied. Most research and reviews on cell adhesion and migration focus on the actin cytoskeleton and its reorganization. However, metastasizing cancer cells undergo the extensive reorganization of their cytoskeletal system, specifically in originating/nucleation sites of microtubules and their orientation (e.g., from non-centrosomal to centrosomal microtubule organizing centers). The precise mechanisms by which the spatial and temporal reorganization of microtubules are linked functionally with the acquisition of an adhesive and migratory phenotype as epithelial cells reversibly transition into mesenchymal cells during metastasis remains poorly understood. In this Special Issue of “Molecular Mechanisms Underlying Cell Adhesion and Migration”, we highlight cell adhesion and migration from the perspectives of microtubule cytoskeletal reorganization, cell polarity and phosphoinositide signaling.

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Section: Distinct Orientation and Organization Of Microtubules In Epi...mentioning

confidence: 99%

Section: Reversion Of Mtoc From Non-centrosomal To Centrosomal Positi...mentioning

confidence: 99%

Section: Reversion Of Mtoc From Non-centrosomal To Centrosomal Positi...mentioning

confidence: 99%

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Regulation of Cell Adhesion and Migration via Microtubule Cytoskeleton Organization, Cell Polarity, and Phosphoinositide Signaling

Thapa,

Wen,

Cryns

et al. 2023

Biomolecules

View full text Add to dashboard Cite

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Transcriptomic profiling of reward and sensory brain areas in perinatal fentanyl exposed juvenile mice

Olusakin

Kumar

Basu

et al. 2023

Neuropsychopharmacol.

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Use of the synthetic opioid fentanyl increased ~300% in the last decade, including among women of reproductive ages. Adverse neonatal outcomes and long-term behavioral disruptions are associated with perinatal opioid exposure. Our previous work demonstrated that perinatal fentanyl exposed mice displayed enhanced negative affect and somatosensory circuit and behavioral disruptions during adolescence. However, little is known about molecular adaptations across brain regions that underlie these outcomes. We performed RNA-sequencing across three reward and two sensory brain areas to study transcriptional programs in perinatal fentanyl exposed juvenile mice. Pregnant dams received 10μg/ml fentanyl in the drinking water from embryonic day 0 (E0) through gestational periods until weaning at postnatal day 21 (P21). RNA was extracted from nucleus accumbens (NAc), prelimbic cortex (PrL), ventral tegmental area (VTA), somatosensory cortex (S1) and ventrobasal thalamus (VBT) from perinatal fentanyl exposed mice of both sexes at P35. RNA-sequencing was performed, followed by analysis of differentially expressed genes (DEGs) and gene co-expression networks. Transcriptome analysis revealed DEGs and gene modules significantly associated with exposure to perinatal fentanyl in a sex-wise manner. The VTA had the most DEGs, while robust gene enrichment occurred in NAc. Genes enriched in mitochondrial respiration were pronounced in NAc and VTA of perinatal fentanyl exposed males, extracellular matrix (ECM) and neuronal migration enrichment were pronounced in NAc and VTA of perinatal fentanyl exposed males, while genes associated with vesicular cycling and synaptic signaling were markedly altered in NAc of perinatal fentanyl exposed female mice. In sensory areas from perinatal fentanyl exposed females, we found alterations in mitochondrial respiration, synaptic and ciliary organization processes. Our findings demonstrate distinct transcriptomes across reward and sensory brain regions, with some showing discordance between sexes. These transcriptome adaptations may underlie structural, functional, and behavioral changes observed in perinatal fentanyl exposed mice.

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Transcriptomic profiling of reward and sensory brain areas in perinatal fentanyl exposed juvenile mice

Olusakin

Kumar

Basu

et al. 2022

Preprint

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BACKGROUND: Use of the synthetic opioid fentanyl increased ~300% in the last decade, including among women of reproductive ages. Adverse neonatal outcomes and long-term behavioral disruptions are associated with perinatal opioid exposure. Our previous work demonstrated that perinatal fentanyl exposed (PFE) mice displayed enhanced negative affect and somatosensory circuit and behavioral disruptions during adolescence. However, little is known about molecular adaptations across brain regions that underlie these outcomes. We performed RNA-sequencing across three reward and two sensory brain areas to study transcriptional programs in PFE juvenile mice. METHODS: C57BL/6 pregnant dams received 10ug/ml fentanyl in the drinking water from embryonic day 0 (E0) through gestational periods until weaning at postnatal day 21 (P21). RNA was extracted from nucleus accumbens (NAc), prelimbic cortex (PrL), ventral tegmental area (VTA), somatosensory cortex (S1) and ventrobasal thalamus (VBT) from PFE mice of both sexes at P35. RNA-sequencing was performed, followed by analysis of differentially expressed genes (DEGs) and gene co-expression networks. RESULTS: Transcriptome analysis revealed sex-specific DEGs and gene modules significantly associated with PFE. The VTA had the most DEGs, while pronounced gene enrichment occurred in NAc. Genes enriched in mitochondrial respiration were pronounced in NAc and VTA of PFE males, extracellular matrix (ECM) and neuronal migration enrichment were pronounced in NAc and VTA of PFE males, while genes associated with vesicular cycling and synaptic signaling were markedly altered in NAc of PFE female mice. In sensory areas from PFE females, we found alterations in mitochondrial respiration, synaptic and ciliary organization processes. CONCLUSION: Our findings demonstrate sex-specific neuroadaptations in PFE mice. Our studies identify distinct transcriptomes across reward and sensory brain regions that may underlie disrupted behavioral and circuit states in PFE mice.

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Organization of cortical microtubules in differentiated cells

Cited by 3 publications

References 48 publications

Regulation of Cell Adhesion and Migration via Microtubule Cytoskeleton Organization, Cell Polarity, and Phosphoinositide Signaling

Regulation of Cell Adhesion and Migration via Microtubule Cytoskeleton Organization, Cell Polarity, and Phosphoinositide Signaling

Transcriptomic profiling of reward and sensory brain areas in perinatal fentanyl exposed juvenile mice

Transcriptomic profiling of reward and sensory brain areas in perinatal fentanyl exposed juvenile mice

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