Organization of cytoskeleton controls the changes in cytosolic calcium of cold-shocked Nicotiana plumbaginifolia protoplasts

Mazars, Christian; Thion, Laurence; Thuleau, Patrice; Graziana, Annick; Knight, Marc R.; Moreau, Marc; Ranjeva, Raoul

doi:10.1016/s0143-4160(97)90025-7

Cited by 88 publications

(67 citation statements)

References 45 publications

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“…Work by Mazars et al (1997) has shown that the cold-induced Ca 2ϩ influx was stimulated by disruption of microtubules and actin microfilaments. Coldinduced actin reorganization may also regulate ion movements across the membrane (Orvar et al, 2000), and cold has been shown to affect stability of the microtubule cytoskeleton in a number of plants (Mizuno, 1992).…”

Section: Discussionmentioning

confidence: 99%

Mutations in the Gravity Persistence Signal Loci in Arabidopsis Disrupt the Perception and/or Signal Transduction of Gravitropic Stimuli

et al. 2002

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Gravity plays a fundamental role in plant growth and development, yet little is understood about the early events of gravitropism. To identify genes affected in the signal perception and/or transduction phase of the gravity response, a mutant screen was devised using cold treatment to delay the gravity response of inflorescence stems of Arabidopsis. Inflorescence stems of Arabidopsis show no response to gravistimulation at 4°C for up to 3 h. However, when gravistimulated at 4°C and then returned to vertical at room temperature (RT), stems bend in response to the previous, horizontal gravistimulation (H. Fukaki, H. Fujisawa, M. Tasaka [1996] Plant Physiology 110: 933-943). This indicates that gravity perception, but not the gravitropic response, occurs at 4°C. Recessive mutations were identified at three loci using this cold effect on gravitropism to screen for gravity persistence signal (gps) mutants. All three mutants had an altered response after gravistimulation at 4°C, yet had phenotypically normal responses to stimulations at RT. gps1-1 did not bend in response to the 4°C gravity stimulus upon return to RT. gps2-1 responded to the 4°C stimulus but bent in the opposite direction. gps3-1 over-responded after return to RT, continuing to bend to an angle greater than wild-type plants. At 4°C, starch-containing statoliths sedimented normally in both wild-type and the gps mutants, but auxin transport was abolished at 4°C. These results are consistent with GPS loci affecting an aspect of the gravity signal perception/transduction pathway that occurs after statolith sedimentation, but before auxin transport.Gravity is a constant stimulus governing the orientation of plant growth. In response to changes in the gravity vector, plants reorient by differential growth. When placed horizontally, shoots and roots of a plant exhibit asymmetric growth resulting in upward or downward curvature, respectively. Gravitropism is only one part of a complex response network that integrates information from developmental and environmental stimuli (Ramussen, 1995). For simplicity, the response pathway has been separated into three sequential steps: gravity perception, signal transduction, and asymmetric growth response (for review, see Sack, 1991). The first step in gravity perception is the sedimentation of amyloplasts in shoots and roots (Sack, 1991;Kiss, 2000;Weise et al., 2000).Later aspects of the gravity response are driven by the asymmetric distribution of auxin that is thought to induce the differential growth that is required for gravitropic curvature (for review, see Muday, 2001). What is much less clear are the mechanisms by which statolith sedimentation initiates the transduction of the gravitropic signal and directs the asymmetric distribution of auxin.Arabidopsis has emerged as a powerful genetic model and has been useful in the dissection of the molecular mechanisms of the gravity response (Tasaka et al., 1999;Chen et al., 2002). Arabidopsis mutants with gravitropic response defects specific to the inflorescence stem, ...

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Section: Discussionmentioning

confidence: 99%

Mutations in the Gravity Persistence Signal Loci in Arabidopsis Disrupt the Perception and/or Signal Transduction of Gravitropic Stimuli

et al. 2002

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“…In cell types other than SEs, the cytoskeleton is involved in the response to local environmental stimuli such as cold shocks (Mazars et al, 1997). Here, we investigated the impact of cytoskeleton disruptors on voltage transients following local cold shocks in SEs, which are associated with Ca 2+ -dependent sieve plate occlusion by forisome dispersion (Thorpe et al, 2010).…”

Section: Involvement Of the Se Cytoskeleton In Local Electrical Respomentioning

confidence: 99%

“…therein). Cytoskeleton disruptors that destabilize either F-actin (Liu and Luan, 1998;Wang et al, 2004;Zhang et al, 2007) or microtubules (Thion et al, 1998) affect the action of ion channels, as demonstrated by patch clamping (Liu and Luan, 1998;Thion et al, 1998;Wang et al, 2004;Zhang et al, 2007) or Ca 2+ fluorescence imaging (Mazars et al, 1997;Wang et al, 2004).…”

Section: Effects Of Lata and Cytd On Ca 2+ Influx And Forisome Conformentioning

confidence: 99%

“…therein;Thuleau et al, 1998;Örvar et al, 2000;Sangwan et al, 2001;Drøbak et al, 2004;Davies and Stankovic, 2006), as in animal cells (Janmey, 1998;Lange and Gartzke, 2006). For instance, K + fluxes are regulated by actin dynamics (Hwang et al, 1997;Liu and Luan, 1998;Chérel, 2004), while Ca 2+ influx into the cytoplasm appears to be mediated by voltage-dependent Ca 2+ -permeable channels associated with microtubules (Mazars et al, 1997;Thion et al, 1998) or by mechanosensitive channels possibly associated with microfilaments (Wang et al, 2004;Zhang et al, 2007). Both types of Ca 2+ -permeable channels probably reside in the SE plasma membrane (Knoblauch et al, 2001;Hafke et al, 2007Hafke et al, , 2009Furch et al, 2009), where they are likely involved in Ca 2+ -dependent systemic signaling Hafke et al, 2009;van Bel et al, 2011;Hafke and van Bel, 2013).…”

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confidence: 99%

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Involvement of the Sieve Element Cytoskeleton in Electrical Responses to Cold Shocks

Hafke¹,

Ehlers

Föller

et al. 2013

Plant Physiology

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This study dealt with the visualization of the sieve element (SE) cytoskeleton and its involvement in electrical responses to local cold shocks, exemplifying the role of the cytoskeleton in Ca 2+ -triggered signal cascades in SEs. High-affinity fluorescent phalloidin as well as immunocytochemistry using anti-actin antibodies demonstrated a fully developed parietal actin meshwork in SEs. The involvement of the cytoskeleton in electrical responses and forisome conformation changes as indicators of Ca 2+ influx was investigated by the application of cold shocks in the presence of diverse actin disruptors (latrunculin A and cytochalasin D). Under control conditions, cold shocks elicited a graded initial voltage transient, DV 1 , reduced by external La 3+ in keeping with the involvement of Ca 2+ channels, and a second voltage transient, DV 2 . Cytochalasin D had no effect on DV 1 , while DV 1 was significantly reduced with 500 nM latrunculin A. Forisome dispersion was triggered by cold shocks of 4°C or greater, which was indicative of an all-or-none behavior. Forisome dispersion was suppressed by incubation with latrunculin A. In conclusion, the cytoskeleton controls cold shock-induced Ca 2+ influx into SEs, leading to forisome dispersion and sieve plate occlusion in fava bean (Vicia faba).

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“…A reasonable amount of knowledge has accumulated about events upstream of cold-induced cytosolic calcium elevations. There appears to be a role for membrane fluidity and the cytoskeleton in cold sensing in plants (Mazars et al, 1997;Orvar et al, 2000;Sangwan et al, 2001). This is in some way coupled to influx of calcium from outside the cell (Monroy and Dhindsa, 1995;Knight et al, 1996), and also IP 3 and cADPR-mediated calcium release from internal stores (Knight et al, 1996;Wu et al, 1997).…”

mentioning

confidence: 99%

Calmodulin as a Potential Negative Regulator of ArabidopsisCOR Gene Expression

Townley

Knight

2002

Plant Physiology

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Organization of cytoskeleton controls the changes in cytosolic calcium of cold-shocked Nicotiana plumbaginifolia protoplasts

Cited by 88 publications

References 45 publications

Mutations in the Gravity Persistence Signal Loci in Arabidopsis Disrupt the Perception and/or Signal Transduction of Gravitropic Stimuli

Mutations in the Gravity Persistence Signal Loci in Arabidopsis Disrupt the Perception and/or Signal Transduction of Gravitropic Stimuli

Involvement of the Sieve Element Cytoskeleton in Electrical Responses to Cold Shocks

Calmodulin as a Potential Negative Regulator of ArabidopsisCOR Gene Expression

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