Organization of pontocerebellar projections to identified climbing fiber zones in the rat

Pijpers, Angelique; Ruigrok, Tom J. H.

doi:10.1002/cne.20940

Cited by 32 publications

(39 citation statements)

References 46 publications

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“…Note that, for the LRn, the ratio of the number of ipsilaterally and contralaterally labeled neurons varies only marginally between different cases, whereas notable differences are observed for the Pn. Data for Pn labeling are in part taken from Pijpers and Ruigrok (2006). cerebellar lobules as climbing fiber collaterals; (2) collaterals of labeled climbing fibers are always accompanied by labeled mossy fiber terminals in the subjacent granular layer; (3) additional mossy fiber collaterals terminate in specific, nonadjacent, striplike regions, which usually have the same zebrin signature as the source of the collateralization; and (4) mossy fiber projections to different cortical zones are accompanied by significant differences in projection densities from two major brainstem sources.…”

Section: Discussionmentioning

confidence: 99%

“…Figure 3A shows a 3D reconstruction of lobule V, SL, and the anterior part of crus 1 for case 979 (see (Voogd et al, 2003;Pijpers and Ruigrok, 2006). Note that only the positive zebrin bands are indicated by their respective numbers and letters.…”

Section: Distribution Of Climbing and Mossy Fiber Collateral Terminalmentioning

confidence: 99%

“…However, lobular differences may also be present. To examine the possibility of zonal and lobular differences more fully, the distribution of retrograde cell labeling in two major sources of mossy fibers was studied: the Pn [based mainly on data recently published by Pijpers and Ruigrok (2006)] and also the LRn.…”

Section: Origin Of Mossy Fibers To Different Lobules and Zonesmentioning

confidence: 99%

“…Consequently, every CTb injection site will result in retrogradely labeled neurons located within a specific combination of these precerebellar centers. We have concentrated our analysis on the LRn and the Pn (Pijpers and Ruigrok, 2006) and show that the distribution of neurons within both structures correlates closely with the lobular identity of the injection site. However, at best only subtle differences were noted in the distribution of labeled neurons arising from injections targeted at different zones within the same lobule.…”

Section: Somatotopy Zones and Origin Of Mossy Fibersmentioning

confidence: 99%

“…Counts of labeled neurons within the basal pontine nuclei (Pn) were constructed in a similar way (cases 864 and AP10). Additional pontine data were also obtained from Pijpers and Ruigrok (2006).…”

Section: Introductionmentioning

confidence: 99%

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Precise Spatial Relationships between Mossy Fibers and Climbing Fibers in Rat Cerebellar Cortical Zones

Pijpers¹,

Apps²,

Pardoe³

et al. 2006

J. Neurosci.

126

132

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Classically, mossy fiber and climbing fiber terminals are regarded as having very different spatial distributions in the cerebellar cortex. However, previous anatomical studies have not studied these two major cerebellar inputs with sufficient resolution to confirm this assumption. Here, we examine the detailed pattern of collateralization of both types of cerebellar afferent using small injections of the bidirectional tracer cholera toxin b subunit into the posterior cerebellum. The cortical and zonal location of these injections was characterized by mapping climbing fiber field potentials, the distribution of retrogradely labeled olivary neurons, and the intrinsic zebrin pattern of Purkinje cells.Labeled climbing fiber collaterals were distributed as longitudinal strips and were always accompanied by clusters of labeled mossy fiber rosettes in the subjacent granular layer. Two-and three-dimensional reconstructions and quantitative analysis showed that mossy fibers also collateralized to other stripe-like regions usually below Purkinje cells with the same zebrin-positive or zebrin-negative characteristics as that of the injection site and associated climbing fiber collaterals. The distribution of retrogradely labeled neurons in two major sources of mossy fibers, the lateral reticular and basilar pontine nuclei, revealed interlobular and some interzonal differences.These data indicate that nonadjacent cerebellar zones, sharing the same climbing fiber input and zebrin identity, also share a common mossy fiber input. Other cerebellar cortical regions that receive collaterals from the same mossy fibers usually also have the same zebrin signature. Together with the distribution of neurons in precerebellar centers, the findings suggest a revision of the modular hypothesis for information processing in the cerebellar cortex.

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Section: Discussionmentioning

confidence: 99%

Section: Distribution Of Climbing and Mossy Fiber Collateral Terminalmentioning

confidence: 99%

Section: Origin Of Mossy Fibers To Different Lobules and Zonesmentioning

confidence: 99%

Section: Somatotopy Zones and Origin Of Mossy Fibersmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Precise Spatial Relationships between Mossy Fibers and Climbing Fibers in Rat Cerebellar Cortical Zones

Pijpers¹,

Apps²,

Pardoe³

et al. 2006

J. Neurosci.

126

132

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Relationship of complex spike synchrony bands and climbing fiber projection determined by reference to aldolase C compartments in crus IIa of the rat cerebellar cortex

Sugihara

Marshall

Lang

2007

J of Comparative Neurology

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Synchronous complex spike (CS) activity occurs most often among cerebellar Purkinje cells located in a narrow longitudinal (parasagittal) strip of cortex (synchrony band). The relationship of the anatomical organization of the olivocerebellar projection to these synchrony bands has not been investigated in detail. Thus, we studied this relationship by using the aldolase C (zebrin II) expression pattern, another landmark for the cerebellar longitudinal organization, as a reference frame in rat crus IIa. Crus IIa consists of 10 aldolase C-positive and -negative longitudinal compartments. Aldolase C labeling after multiple-electrode recording of CSs indicated that in lateral crus IIa (compartments 5+ to 7+) synchrony bands were generally constrained to single compartments. In contrast, in medial crus IIa (compartments 4a- to 5a-) the synchrony within and across the compartments was much higher than in lateral crus IIa, resulting in wide synchrony bands covering multiple compartments. Retrograde labeling of olivary neurons by injections of biotinylated dextran amine into aldolase C compartments in crus IIa showed that compartments in medial crus IIa were all innervated by the caudal part of the medial accessory olive. On the other hand, each aldolase C compartment in the lateral crus IIa was innervated by a region in a different subnucleus in the rostral inferior olive. These regions in different subnuclei were located close to each other. These results suggest that CS synchrony bands reflect the olivocerebellar compartmental projection pattern and neuronal coupling within a particular olivary subnucleus, and that medial and lateral crus IIa may be functionally distinct.

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Projection patterns of single mossy fiber axons originating from the dorsal column nuclei mapped on the aldolase C compartments in the rat cerebellar cortex

Quy

Fujita

Sakamoto

et al. 2011

J of Comparative Neurology

116

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Although cerebellar mossy fibers are the most abundant cerebellar afferents and are deeply involved in cerebellar function, the organization of their projection has remained obscure, particularly in relation to cerebellar compartmentalization. The dorsal column nuclei (DCN) are a major source of cerebellar mossy fibers and possess distinct somatotopic representations of specific somatosensory submodalities. We reconstructed individual dextran-labeled DCN axons completely from serial sections and mapped their terminals on the longitudinal cerebellar compartments that were visualized by aldolase C immunostaining to clarify their projection pattern. Individual axons branched and formed about 100 rosette terminals in the cerebellar cortex, but infrequently projected to the cerebellar nuclei (1 out of 15 axons). Cortical terminals were clustered in multiple areas in the vermis and pars intermedia mostly, but not exclusively, ipsilateral to the origin of the axon. The gracile, cuneate, and external cuneate nuclei (ECuN) mainly projected to the copula pyramidis and lobule V, paramedian and simple lobules, and lobules I-V and VIII-IX, respectively, although there was some overlap. The majority of terminals were located within aldolase C negative or lightly positive compartments. However, terminals of a single axon can be located on aldolase C-negative as well as on aldolase C-positive compartments. In particular, the rostral ECuN, which is responsive to shoulder movements, projected consistently to lobule IX, which were mostly aldolase C-positive. In sum, DCN-cerebellar axons project to multiple compartments with terminals clustered mainly in the conventional spinocerebellar region with a coarse topography, which shows some relationship to the cortical compartments defined by aldolase C.

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Organization of pontocerebellar projections to identified climbing fiber zones in the rat

Cited by 32 publications

References 46 publications

Precise Spatial Relationships between Mossy Fibers and Climbing Fibers in Rat Cerebellar Cortical Zones

Precise Spatial Relationships between Mossy Fibers and Climbing Fibers in Rat Cerebellar Cortical Zones

Relationship of complex spike synchrony bands and climbing fiber projection determined by reference to aldolase C compartments in crus IIa of the rat cerebellar cortex

Projection patterns of single mossy fiber axons originating from the dorsal column nuclei mapped on the aldolase C compartments in the rat cerebellar cortex

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