Organization of the Bacterial Chromosome

Schumann, Wolfgang

doi:10.1002/3527608494.ch2

Cited by 2 publications

(2 citation statements)

References 159 publications

(192 reference statements)

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“…This process is successively repeated by F3-F5. Unlike in mammals, however, all follicles within this cohort do not undergo atresia [24]. The prehierarchal follicles are categorized by size (2-4, 4-6, 6-8, and 8-10 mm in diameter).…”

Section: Introductionmentioning

confidence: 99%

Metabolomic Analysis of SCD during Goose Follicular Development: Implications for Lipid Metabolism

Yuan

et al. 2020

Genes

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Stearoyl-CoA desaturase (SCD) is known to be an important rate-limiting enzyme in the production of monounsaturated fatty acids (MUFAs). However, the role of this enzyme in goose follicular development is poorly understood. To investigate the metabolic mechanism of SCD during goose follicular development, we observed its expression patterns in vivo and in vitro using quantitative reverse-transcription (qRT)-PCR. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to determine a cellular model of SCD function in granulosa cells (GCs) via SCD overexpression and knockdown. qRT-PCR analysis showed that SCD was abundantly expressed in the GC layer, and was upregulated in preovulatory follicles. Peak expression was found in F1 and prehierarchal follicles with diameters of 4–6 mm and 8–10 mm, respectively. We further found that mRNA expression and corresponding enzyme activity occur in a time-dependent oscillation pattern in vitro, beginning on the first day of GC culture. By LC-MS/MS, we identified numerous changes in metabolite activation and developed an overview of multiple metabolic pathways, 10 of which were associated with lipid metabolism and enriched in both the overexpressed and knockdown groups. Finally, we confirmed cholesterol and pantothenol or pantothenate as potential metabolite biomarkers to study SCD-related lipid metabolism in goose GCs.

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Section: Introductionmentioning

confidence: 99%

Metabolomic Analysis of SCD during Goose Follicular Development: Implications for Lipid Metabolism

Yuan

et al. 2020

Genes

View full text Add to dashboard Cite

show abstract

“…This process is successively repeated by F3-F5. Unlike mammals, however, all follicles within this cohort do not undergo atresia [24]. The prehierarchal follicles are categorized by size (2-4, 4-6, 6-8, and 8-10 mm in diameter).…”

Section: Introductionmentioning

confidence: 99%

Metabolomic Analysis of SCD During Goose Follicular Development: Implications for Lipid Metabolism

Yuan

et al. 2020

Preprint

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Background Stearoyl-CoA desaturase (SCD) is known to be an important rate-limiting enzyme in the production of MUFA. The role of this enzyme in goose follicular development is poorly understood. To investigate the metabolic mechanism of SCD during goose follicular development, we observed SCD expression patterns during follicular development in vivo and in vitro using quantitative reverse-transcription (qRT)-PCR. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to determine a cellular model of SCD function in granulosa cells (GCs) via SCD overexpression and knockdown.Results qRT-PCR analysis showed that SCD was abundantly expressed in the GC layer, and was upregulated in preovulatory follicles. Peak expression was found in F1 and prehierarchal follicles with diameters of 4–6 mm and 8–10 mm, respectively. We further found the mRNA expression and corresponding enzyme activity to occur in a time-dependent oscillation in vitro, beginning on the first day of GC culture. By using LC-MS/MS, we identified numerous changes in metabolite activation and developed an overview of multiple metabolic pathways, ten of which were associated with lipid metabolism and enriched in both the overexpressed and the knockdown groups.ConclusionsWe confirmed cholesterol and pantothenol or pantothenate as potential metabolite biomarkers for studying SCD-related lipid metabolism in goose GCs.

show abstract

Organization of the Bacterial Chromosome

Cited by 2 publications

References 159 publications

Metabolomic Analysis of SCD during Goose Follicular Development: Implications for Lipid Metabolism

Metabolomic Analysis of SCD during Goose Follicular Development: Implications for Lipid Metabolism

Metabolomic Analysis of SCD During Goose Follicular Development: Implications for Lipid Metabolism

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