Organization of the chloroplast ribosomal RNA genes of Euglena gracilis bacillaris

Helling, Robert B.; El-Gewely, M. Raafat; Lomax, Margaret I.; Baumgartner, James E.; Schwartzbach, Steven D.; Barnett, W. Edgar

doi:10.1007/bf00433298

Cited by 31 publications

(29 citation statements)

References 25 publications

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“…Very few plants have only one operon (20). In contrast, the number of rrn operons in the chloroplast genomes of Euglenagracilis fluctuates between one in the Z-S strain, three in the Z and the bacillaris strains, and five in the bacillaris-ATCC strain (21,16,7,13). The tandem arrangement of repeated units seems to facilitate the amplification or reduction of these units.…”

Section: Discussionmentioning

confidence: 99%

“…Euglena gracilis strain bacillaris was obtained from J. Schiff through W. E. Barnett and has been described, (7). Euglena gracilis strain Z-S was obtained from D.E.…”

Section: Methodsmentioning

confidence: 99%

“…Strain Z and strain bacillaris (16,7) contain three operons, whereas the strains Z-S and bacillaris-ATCC contain one and five operons, respectively (21,13). In addition the presence of one, and of two extra 16S rRNA genes was demonstrated for the Z and for the bacillaris-ATCC strains, respectively (9,13).…”

Section: Introductionmentioning

confidence: 99%

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Structure and rearrangements of rRNA genes in chloroplast DNA in two strains of Euglena gracilis

Koller¹,

Delius²,

Helling

1984

Plant Mol Biol

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The organisation of the rRNA genes in the chloroplast genomes of two strains of Euglena gracilis were analyzed and compared. It was previously shown that the bacillaris strain contains three complete rrn (rRNA) operons (7) and that the Z-S strain contains one operon (21). Using heteroduplex analysis it was found that the bacillaris strain contains, apart from the three complete rrn operons, an extra 16S rRNA gene, an extra partial 23S rRNA gene sequence and an inverted duplication of a stretch within the 5S-16S spacer. In addition a short (<100 bp) inverted repeat sequence (13) which forms a stem/loop structure in single-stranded cpDNA was located between the 3'-end of the extra 16S rRNA gene and the partial 23 S rRNA sequence.The Z-S strain differs from the bacillaris strain by a deletion of two units of the complete rrn operons. The region upstream of the single complete rrn operon, including the inverted repeats, the partial 23S and the extra 16S rRNA sequences is identical with the bacillaris strain.The only non-homology found in heteroduplexes between the SalI fragments of B of the two strains is the deletion-insertion loop which represents the two rrn operons. A small deletion loop was found occasionally in hetero-and in homoduplexes of both strands in the region of variable size. Apart from the deletion/insertion of two rrn operons the two genomes appear to be colinear as can be seen from partial denaturation mapping. The organisation of the rRNA genes of the two strains is compared with those of the Z strain and the bacillaris-ATCC strain.

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Section: Discussionmentioning

confidence: 99%

“…Euglena gracilis strain bacillaris was obtained from J. Schiff through W. E. Barnett and has been described, (7). Euglena gracilis strain Z-S was obtained from D.E.…”

Section: Methodsmentioning

confidence: 99%

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Structure and rearrangements of rRNA genes in chloroplast DNA in two strains of Euglena gracilis

Koller¹,

Delius²,

Helling

1984

Plant Mol Biol

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“…Both two genes (Tewari, 1979) a n d o n e g e n e (Palmer a n d T h o m p s o n , 1981) have been r e p o r t e d for the pea. Euglena gracilis presents an interesting case in that the fine structure of the r R N A genes o n its chloroplast g e n o m e is quite variable (Helling et aL, 1979; W u r t z a n d . Euglena gracilis strains Ζ a n d bacillans both have t h r e e genes (Gray a n d ; J e n n i a n d ; R a w s o n^a L , 1978; H e l l i n g^a L , 1979; W u r t z a n d , but differ in the size of the spacer region between the 16 S a n d 23 S r R N A genes.…”

Section: Genes For Chloroplast Ribosomal Ribonucleic Acidsmentioning

confidence: 99%

“…long in maize , 1680 b.p. I n Euglena gracilis bacillaris, the t h r e e sets of r R N A genes differ in length a n d sequence of their own spacers a n d with the spacers of strain Ζ (Helling et al, 1979; W u r t z a n d . in spinach (Whitfeld et al, 1978a; B o h n e r t et al, 1979) a n d 2 5 0 -3 0 0 b.p.…”

Section: Genes For Chloroplast Ribosomal Ribonucleic Acidsmentioning

confidence: 99%