Organization of Thylakoid Membranes in Low-Light Grown Maize Seedlings. Effect of Lincomycin Treatment

Nyitrai, Péter; Sárvári, Éva; Keresztes, Attila; Láng, F.

doi:10.1016/s0176-1617(11)81201-0

Cited by 15 publications

(9 citation statements)

References 25 publications

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“…Chl content and Chl a/b ratios of leaves were determined according to Porra et al (1989). Thylakoid membranes were isolated as in Nyitrai et al (1994). Solubilisation of the membranes and separation of CPCs were carried out according to Sµrvµri and Nyitrai (1994).…”

Section: Methodsmentioning

confidence: 99%

Rejuvenation of Ageing Bean Leaves under the Effect of Low‐Dose Stressors

et al. 2004

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The effect of low concentrations of some stress-inducing compounds like Cd, Pb, Ni, and Ti salts and DCMU on the senescence of chloroplasts was investigated in detached primary leaves of bean. After the petioles of ageing leaves had developed roots, these low-dose stressors stimulated chlorophyll synthesis and photosynthetic activity, as compared to the control, thus causing rejuvenation in treated leaves. The amount of photosystem I (lowest in DCMU-treated leaves) and light-harvesting complex II increased, while that of photosystem II decreased or remained unchanged. Fluorescence induction parameters indicated unchanged electron transport (except for DCMU treatment). CO2 fixation and, in some cases, starch accumulation was stimulated. In parallel, the occurrence of large plastoglobuli seemed to decrease in plastids of heavy metal-treated leaves. A cytokinin bioassay of leaf extracts confirmed the cytokinin-mediated effect of low-dose stressors, as the slopes of Chl and cytokinin curves were similar during the rejuvenation process. It is assumed that these stressors generate non-specific alarm reactions, which involve changes in the hormonal balance by increasing the synthesis of cytokinins.

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Section: Methodsmentioning

confidence: 99%

Rejuvenation of Ageing Bean Leaves under the Effect of Low‐Dose Stressors

et al. 2004

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“…In addition, shade stress induces more superoxide, H 2 O 2 and hydroxyl radicals in plants [19]. Plants alter morphological and nutrients distribution to accommodate lack light, such as adapting to low-light environments by synthesizing more chlorophyll as part of the light-harvesting complex [20][21][22]. Increased light results in a denser thylakoid layer structure, and significantly enhanced photosystem I and II (PSI and PSII) activity [23,24].…”

Section: Introductionmentioning

confidence: 99%

Physiological and comparative proteomic analysis provides new insights into the effects of shade stress in maize (Zea mays L.)

et al. 2020

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Background: Shade stress, a universal abiotic stress, suppresses plant growth and production seriously. However, little is known regarding the protein regulatory networks under shade stress. To better characterize the proteomic changes of maize leaves under shade stress, 60% shade (S) and supplementary lighting (L) on cloudy daylight from tasseling stage to physiological maturity stage were designed, the ambient sunlight treatment was used as control (CK). Isobaric tag for relative and absolute quantification (iTRAQ) technology was used to determine the proteome profiles in leaves. Results: Shading significantly decreased the SPAD value, net photosynthetic rate, and grain yield. During two experimental years, grain yields of S were reduced by 48 and 47%, and L increased by 6 and 11%, compared to CK. In total, 3958 proteins were identified by iTRAQ, and 2745 proteins were quantified including 349 proteins showed at least 1.2-fold changes in expression levels between treatments and CK. The differentially expressed proteins were classified into photosynthesis, stress defense, energy production, signal transduction, and protein and amino acid metabolism using the Web Gene Ontology Annotation Plot online tool. In addition, these proteins showed significant enrichment of the chloroplasts (58%) and cytosol (21%) for subcellular localization. Conclusions: 60% shade induced the expression of proteins involved in photosynthetic electron transport chain (especially light-harvesting complex) and stress/defense/detoxification. However, the proteins related to calvin cycle, starch and sucrose metabolisms, glycolysis, TCA cycle, and ribosome and protein synthesis were dramatically depressed. Together, our results might help to provide a valuable resource for protein function analysis and also clarify the proteomic and physiological mechanism of maize underlying shade stress.

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“…In studies of various aspects of photoinhibition, investigators have employed lincomycin treatment during measurements (11)(12)(13)(14)(15)(16); however, little is known about the long-term application of lincomycin and its consequences on plant growth and photosynthetic membrane development. Early work that involved relatively short treatments with plastid protein synthesis inhibitors in greening beans and maize seedlings suggested a relative decrease in RCIIs and structural alterations that manifested as changes in the distribution and abundance of the photosynthetic membrane proteins (17,18).…”

Section: Introductionmentioning

confidence: 99%

Higher Plant Photosystem II Light-Harvesting Antenna, Not the Reaction Center, Determines the Excited-State Lifetime—Both the Maximum and the Nonphotochemically Quenched

Belgio

Johnson

Jurić

et al. 2012

Biophysical Journal

129

147

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The maximum chlorophyll fluorescence lifetime in isolated photosystem II (PSII) light-harvesting complex (LHCII) antenna is 4 ns; however, it is quenched to 2 ns in intact thylakoid membranes when PSII reaction centers (RCIIs) are closed (Fm). It has been proposed that the closed state of RCIIs is responsible for the quenching. We investigated this proposal using a new, to our knowledge, model system in which the concentration of RCIIs was highly reduced within the thylakoid membrane. The system was developed in Arabidopsis thaliana plants under long-term treatment with lincomycin, a chloroplast protein synthesis inhibitor. The treatment led to 1), a decreased concentration of RCIIs to 10% of the control level and, interestingly, an increased antenna component; 2), an average reduction in the yield of photochemistry to 0.2; and 3), an increased nonphotochemical chlorophyll fluorescence quenching (NPQ). Despite these changes, the average fluorescence lifetimes measured in Fm and Fm' (with NPQ) states were nearly identical to those obtained from the control. A 77 K fluorescence spectrum analysis of treated PSII membranes showed the typical features of preaggregation of LHCII, indicating that the state of LHCII antenna in the dark-adapted photosynthetic membrane is sufficient to determine the 2 ns Fm lifetime. Therefore, we conclude that the closed RCs do not cause quenching of excitation in the PSII antenna, and play no role in the formation of NPQ.

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Organization of Thylakoid Membranes in Low-Light Grown Maize Seedlings. Effect of Lincomycin Treatment

Cited by 15 publications

References 25 publications

Rejuvenation of Ageing Bean Leaves under the Effect of Low‐Dose Stressors

Rejuvenation of Ageing Bean Leaves under the Effect of Low‐Dose Stressors

Physiological and comparative proteomic analysis provides new insights into the effects of shade stress in maize (Zea mays L.)

Higher Plant Photosystem II Light-Harvesting Antenna, Not the Reaction Center, Determines the Excited-State Lifetime—Both the Maximum and the Nonphotochemically Quenched

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