Theoretical model of system determination of subjective well-being at work at the levels of society culture, organizational culture, personality, and psychophysiological level is presented. Subjective well-being at work is considered as a necessary condition for adult working man to experience happiness. The results of research of subjective well-being of personnel of Russian companies (N=425) are presented. It is shown that subjective well-being can be considered as a social action of interactive nature. The key role of organizational culture was revealed. The following indicators of subjective well-being are considered: conformity of individual values of organizational development with the vector of organizational culture, the level of organizational stress, self-assessment of fatigue and health. The role of subjective well-being in the conditions of organizational changes as an emotional regulator of personnel’s acceptance of innovations is shown. The effects of subjective well-being include personal involvement in work activities, age-related self-esteem, and personal activity in working with information. The analysis of characteristics of modern economy, which allow employees of companies to acquire meanings of acceptance of innovations in corporate training, is given. The managerial practices contributing to the construction of a culture of resilience are shown.