Organo-silica nano-particles used in ultrahigh-pressure liquid chromatography

Cintrón, José M.; Colón, Luis A.

doi:10.1039/b203236h

Cited by 63 publications

(53 citation statements)

References 17 publications

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“…Foi observado que ambas as colunas apresentaram resultados similares para eficiência da coluna e resistência mecânica em pressão de 4500 bar. Cintron e Colon [84] realizaram a síntese de partí-culas não porosas de sílica, com diâmetro médio de 0,67 µm, e, após a funcionalização com C18, prepararam uma coluna de 90 mm × 50 µm, contendo as partículas sub-1 µm. Empregando um sistema de cromatografia líquida de ultra eficiência (UHPLC) capaz de trabalhar em 3.500 bar Tabela 1 Diminuição do tamanho das partículas em LC e sua influência na eficiência da coluna (N).…”

Section: Perspectivas Futuras No Desenvolvimento De Suportes Cromatogunclassified

Suportes cromatográficos e fases estacionárias para cromatografia líquida: preparo, evolução e tendências

Nazário¹,

Lanças²

2013

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ResumoDesde o surgimento da instrumentação dedicada à HPLC, essa técnica analítica tem se destacado na separação e análise de uma grande variedade de compostos em diferentes campos de atuação. Nesse contexto, a área de pesquisa e desenvolvimento (P&D) de suportes cromatográficos e de fases estacionárias para HPLC tem recebido atenção de pesquisadores, objetivando análises rápidas, colunas eficientes e seletividade adequada. Neste trabalho discutem-se os métodos utilizados no preparo de suportes cromatográficos para HPLC (principalmente os baseados em sílica) e as estratégias de modificação de sua superfície no preparo de fases estacionárias. Palavras-chaveHPLC; suporte cromatográfico; sílica; fase estacionária.Chromatographic supports and stationary phases for liquid chromatography: preparation, evolution and trends AbstractSince the development of instrumentation for HPLC, this analytical technique has been widely employed in the separations and analysis of a large variety of compounds in different fields. In this context, research and development (R & D) area of chromatographic supports and stationary phases for HPLC have been receiving attention from researchers aiming the decrease in analysis time, increase column efficient and promote adequate selectivity. In this paper, we focused on the methods for the preparation of chromatographic supports for HPLC (especially silica-based materials) and strategies for modifying its surface in the preparation of stationary phases.

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Section: Perspectivas Futuras No Desenvolvimento De Suportes Cromatogunclassified

Suportes cromatográficos e fases estacionárias para cromatografia líquida: preparo, evolução e tendências

Nazário¹,

Lanças²

2013

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“…Recently, Cintrón and Colón [46] reported the use of nanometer-sized organo-silica particles in HPLC. According to the results summarized in previous investigations [47], the analysis time decreases and the theoretical plate number increases with decreasing particle diameter, which can be depicted by the following equations:…”

Section: Silica Nanoparticles In Capillary Hplcmentioning

confidence: 99%

Applications of nanomaterials in liquid chromatography: Opportunities for separation with high efficiency and selectivity

Zhang

Wang

Liao

et al. 2006

J of Separation Science

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ReviewApplications of nanomaterials in liquid chromatography: Opportunities for separation with high efficiency and selectivity During recent decades, great efforts have been made to improve the chemical stability, selectivity, and separation efficiency of stationary phases in liquid chromatography. Significant progress has been achieved, especially after the introduction of nanomaterials into separation science. This review covers the applications of nanomaterials playing various roles in liquid chromatography. Future possibilities for developing nanomaterial-based stationary phases are also discussed.

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“…Because these particles operate at a higher pressure than conventional HPLC, certain challenges associated with the particles must be addressed in particle design. In the pioneering ultra-high-pressure LC studies, monodisperse, nonporous particles were used (46)(47)(48). Failed packing attempts (3ϫ lower efficiency) were also reported with porous particles of a wider d p distribution (49).…”

Section: Packing Materialsmentioning

confidence: 99%

“…However, columns with porous packing material with a wide size distribution showed extensive nonuniformity (48). Reduced HETP values close to 2 were reported on small, 30-150-µm-i.d., sub-2-µm-nonporous-particle packed fused silica capillaries under ultra-high-pressure LC conditions with on-column detection (46)(47)(48)(49). On the basis of these studies, it was generally assumed that only monodisperse, nonporous particles with d p distributions <5% RSD are suitable to obtain high efficiency under ultrahigh-pressure conditions.…”

Section: Packing Materialsmentioning

confidence: 99%

Advancing LC Performance with Smaller Particles and Higher Pressure

Mazzeo¹,

Neue²,

Kele³

et al. 2005

Anal. Chem.

298

146

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HPLCremains the workhorse technique in liquidphase separations. The method has two fundamental roots. One is gel-permeation chromatography for the characterization of polymers (1); the other is GC. HPLC began to emerge when GC researchers turned their attention to liquid-phase separations (2, 3). The original LC columns were typically 50-100 cm long, with a 1-2 mm i.d., and packed with 50-200-µm-diam particles. Shorter diffusion distances were known to provide better performance. However, it was not possible to prepare high-performance columns with particles with diameters <30 µm by using the dry-packing techniques that had been applied successfully in GC. One improvement was the development of superficially porous particles that resulted in better column performance at low retention factors (4,5).A breakthrough was achieved in the early 1970s, when slurry packing techniques were developed for fully porous particles <30 µm in diameter (6-8). Researchers rapidly learned to pack efficient columns with 10-, 5-, and 3-µm particles (9-11). The first commercial columns packed with 10-µm particles became available in 1973 (12). During these early investigations, it became clear that the pressures required to operate long, small-particle columns at suitable velocities (above the minimum of the plate-height-velocity curve) heat up the mobile phase because of friction (13,14). This heat would ultimately limit the benefits of using smaller particle sizes unless measures were taken to compensate for this effect.Today, two driving forces continue to test the limits of HPLC. One is the need for faster separations, such as analyses of either simple samples or a few constituents in a complex sample (15-17 ). The second is the desire to achieve greater separation power to quantify or identify all the constituents of a complex sample or to compare the contents of complex samples with each other (18)(19)(20)(21)(22) A new separati on techni que takes advantage of sub-2-µm porous parti cl es.

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Organo-silica nano-particles used in ultrahigh-pressure liquid chromatography

Cited by 63 publications

References 17 publications

Suportes cromatográficos e fases estacionárias para cromatografia líquida: preparo, evolução e tendências

Suportes cromatográficos e fases estacionárias para cromatografia líquida: preparo, evolução e tendências

Applications of nanomaterials in liquid chromatography: Opportunities for separation with high efficiency and selectivity

Advancing LC Performance with Smaller Particles and Higher Pressure

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