Organogenèse induite chez des plants deProsopis farcta(Fabaceae, Mimosoideae) développésin vitropar apport de diverses concentrations en ions Na⁺et SO₄^−-

Induction of organogenesis and callogenesis in Limbarda crithmoïdes L. (Asteraceae) explants cultured on MS media supplemented with various concentrations of Na+ and K+

“…Generally, callogenesis induction without the addition of hormones is scarce. In Tunisia, for Prosopis farcta; halophyte and native species, Harzallah-Skhiri (2003) and Stambouli et al (2011Stambouli et al ( , 2012 revealed that, media containing maximum concentrations of sodium (100, 150 mM) with 0.1 and 20 mM of calcium, or 0.1 mM sodium with 25 mM of sulfate ion promote optimal callus formation on hypocotyl (36 and 84%, respectively). Previously, in Algeria, Benrebiha et al (1992) proved that in the absence of growth substances, callus control is possible by addition of macronutrients in the in vitro culture medium of Atriplex halimus L. They explained that their role is at the cell membrane level through an indirect effect on the internal hormonal balances.…”

“…Besides, research works studying the effect of the mineral balance on in vitro organogenesis are very scarce. From which, we quote works of Harzallah-Skhiri (2003) and Stambouli et al (2011; for the Tunisian native Prosopis farcta (Banks &Soland.) Macbride and that of Benrebiha et al (1992) for Atriplex halimus L. Mineral balance is crucial and have an important impact on the plant in vitro development (Niedz and Evens, 2007;Aranda-Peres et al, 2009;Efferth, 2019).…”