Organogenesis and Ultrastructural Features ofIn VitroGrownCanna indicaL.

Abstract

An efficient protocol for micropropagation of Canna indica L., an economically and pharmaceutically important plant, was standardized using rhizome explants, excised from two-month-old aseptic seedlings. Complete plant regeneration was induced on MS medium supplemented with 3.0 mg/L BAP plus 1.5 mg/L NAA, which produced the highest number of shoots (73.3 ± 0.5%) and roots (86.7 ± 0.4%) after 2 weeks. Furthermore, the optimum media for multiple shoots regeneration were recorded on MS enriched with 7.0 mg/L BAP (33.0 ± 0.5%). Plantlets obtained were transplanted to pots after two months and acclimatized in the greenhouse, with 75% survival. In addition, ultrastructural studies showed that rhizomes of in vitro grown specimens were underdeveloped compared to the in vivo specimens, possibly due to the presence of wide spaces. Meanwhile, the leaves of in vivo specimens had more open stomata compared to in vitro specimens, yet their paracytic stomata structures were similar. Hence, there were no abnormalities or major differences between in vitro regenerants and mother plants.

Medicinal importance of Kaempferia galanga L. (Zingiberaceae): A comprehensive review

“…Kaempferia galanga belonging to the Zingiberaceae family, is one of the potential medicinal plants endemic to India and distributed across Indonesia (7,8), Laos (9), Sri Lanka (10,11), China (12), Malaysia (13,14), Nigeria (15), Thailand (16,17), Bangladesh (18), Japan (19), Sudan (20), and Vietnam (21). In traditional medicine in Asian countries, this plant is widely used by local practitioners (22).…”

Low-cost shoot multiplication and improved growth in different cultivars of Canna indica

“…The present protocol is an improvement over the already reported protocol by Wafa et al (2016), as they used the rhizome explants from the aseptically established seedlings, whereas we have not only used field-grown rhizome explants for multiplication, but all the multiplied shoots grew well also. Use of different strengths and phases (e.g., solid and liquid phases) of MS and other nutrient media for improved in vitro regeneration of shoots in Canna have already been reported in the literature (Kromer and Kukulczanka 1984;Sakai and Imai 2007).…”

“…Recently, there is one review on in vitro regeneration of Canna (Mishra et al 2015) which majorly discussed C. edulis Ker., an edible species native to Andes and South America (Sakai and Imai 2007), and a report on C. indica (Kromer and Kukulczanka 1984). Wafa et al (2016) reported organogenesis from in vitro-grown seedling rhizome explants, as they could not establish in vivo-grown rhizome under aseptic culture. There are only 1-2 reports, where meristem culture techniques were used to get virus-free cannas (Kromer and Kukulczanka 1985).…”

Effects of Light Environments on Leaf Traits and Phenotypic Plasticity of Canna indica

“…There has been much research done on C. indica, covers a broad range of subjects such as its starch quality and physicochemical properties (Algar et al, 2019;Andrade-Mahecha et al, 2012), its bioactive components and phytochemical analysis (Al-Snafi, 2015;Ayusman et al, 2020;Ifandari et al, 2020), its organogenesis and ultrastructural features (Wafa et al, 2016), the effect of water deficiency and nutrients on its growth (Zhang et al, 2008), its floral vasculature and pollination biology (Glinos & Cocucci, 2011;Miao et al, 2014), its use for domestic and industrial wastewater treatments (Subhashini & Swamy, 2014), and its use for biogas production (Jiang et al, 2014). Unfortunately, little is known about C. indica's adaptability to different light environments (see for example Asmelash, 2017 andCiciarelli, 2012).…”