Organogenesis in Camellia x williamsii: cytokinin requirement and susceptibility to antibiotics

Abstract

The genetic transformation of plants calls for efficient organogenetic methods. Both cytokinins and antibiotics were tested to evaluate shoot regeneration from internodes of in vitro plants of Camellia x williamsii cv Debbie. High regeneration rates were achieved by using thidiazuron, which turned out to be more effective than 6-benzylaminopurine. Up to 96% of explants regenerated when thidiazuron was used, whereas no more than 75% regenerated using 6-benzylaminopurine. The best average number of shoots per regenerant explant was 9.7 and 5.6 regarding respectively thidiazuron and 6-benzylaminopurine. Kanamycin, used in combination with the best performing thidiazuron concentration, completely blocked regeneration at 129 μM. Cefotaxime at 524 μM decreased the regeneration ability, especially when 2 day preculture was applied. The application of genetic transformation protocols as well as the main aims of genetic engineering in ornamental camellias are discussed.

Factor affecting Agrobacterium-mediated transformation of rice chitinase gene in Solanum tuberosum L.

“…Sensitivity of plants to antibiotics is species specific and depends on plant growth conditions (Lin et al, 1995;Joersbo and Okkels, 1996;Chauvin et al, 1999). Cefotaxime had negative effect on the regeneration of camellia (Tosca et al, 1996), and tomato (Ling et al, 1998), but had positive effect on callus growth and organogenesis in wheat (Mathias and Boyd, 1986) and Pinus pinea (Humara et al, 1999).…”