Organoids and Liquid Biopsy in Oral Cancer Research

Eguchi, Takanori

doi:10.3390/jcm9113701

Cited by 4 publications

(2 citation statements)

References 33 publications

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“…In recent years, a spheroid model produced by three-dimensional (3D)-culture has attracted attention as a better experimental model that is closer to the physiological environment in vivo [2]. The intra-and extra-cellular events that occur in 3D culture are similar to those in a physiological environment [3][4][5]. Specifically, it is possible to create site-specific Ivyspring International Publisher differences in the cell proliferation rate and cell morphology/function, imitate microenvironmental conditions such as hypoxia and particular nutritional gradients, and predict the biological response to drug treatment [6].…”

Section: Introductionmentioning

confidence: 99%

Reproduction of the Antitumor Effect of Cisplatin and Cetuximab Using a Three-dimensional Spheroid Model in Oral Cancer

Ono¹,

Sato²,

Nakamura³

et al. 2022

Int. J. Med. Sci.

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Background/Aim: Cancer research has been conducted using cultured cells as part of drug discovery testing, but conventional two-dimensional culture methods are unable to reflect the complex tumor microenvironment. On the other hand, three-dimensional cultures have recently been attracting attention as in vitro models that more closely resemble the in vivo physiological environment. The purpose of this study was to establish a 3D culture method for oral cancer and to verify its practicality. Materials and Methods: Three-dimensional cultures were performed using several oral cancer cell lines. Western blotting was used for protein expression analysis of the collected cell masses (spheroids), and H-E staining was used for structural observation. The cultures were exposed to cisplatin and cetuximab and the morphological changes of spheroids over time and the expression changes of target proteins were compared. Results: Each cell line formed spheroidal cell aggregates and showed enhancement of cell adhesion molecules over time. H-E staining showed tumor tissue-like structures specific to each cell line. Cisplatin showed concentration-dependent antitumor effects due to loss of cell adhesion and spheroid disruption in each cell line, while cetuximab exhibited antitumor effects that correlated with EGFR expression in each cell line. Conclusion: Spheroids made from oral cancer cell lines appeared to have tumor-like characteristics that may reflect their clinical significance. In the future, it may become possible to produce tumor spheroids from tissue samples of oral cancer patients, and then apply them to drug screening and to develop individualized diagnostic and treatment methods.

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Section: Introductionmentioning

confidence: 99%

Reproduction of the Antitumor Effect of Cisplatin and Cetuximab Using a Three-dimensional Spheroid Model in Oral Cancer

Ono¹,

Sato²,

Nakamura³

et al. 2022

Int. J. Med. Sci.

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“…Moreover, its direct contact with tumour tissue makes it a preferred diagnostic and prognostic tool in oral cancer research. 5 , 6 …”

Section: Introductionmentioning

confidence: 99%

Expression of miR-31 in saliva-liquid biopsy in patients with oral squamous cell carcinoma

Kumari

Syed

Wahid

et al. 2021

Journal of Taibah University Medical Sciences

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Objectives Oral squamous cell carcinoma (OSCC) is a commonly reported cancer in men and is second only to breast cancer in women in Pakistan.. Investigations for identifying biomarkers of OSCC are essential for diagnostic, therapeutic, or prognostic significance. This study aims to examine the miR-31 expression in the pre- and post-operative OSCC patients and correlate this expression with clinicopathological characteristics. Methods Patients with histopathologically confirmed OSCC who had undergone surgical resections of tumours were recruited. A total of 40 saliva samples (pre- and post-operative) were collected from 19 patients and two healthy individuals. Levels of salivary miR-31 expressions were examined through quantitative reverse transcription polymerase chain reaction. Results The salivary miR-31 expression was significantly higher in the preoperative patients than in postoperative patients ( p < 0.001). However, no significant correlation had been found between the salivary miR-31 expression and clinicopathological characteristics ( p > 0.05). Conclusion Our data suggest that miR-31 can be used as an adjunct non-invasive marker to monitor surgery outcomes during postoperative follow-up in patients with OSCC.

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Oral squamous cell carcinoma

Gulati,

Sobti

2024

Biomarkers in Cancer Detection and Monitoring of Therapeutics

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Organoids and Liquid Biopsy in Oral Cancer Research

Abstract: To promote the newest discoveries in oral cancer research, a special issue “Frontiers in Oral Cancer—Basic and Clinical Sciences” in the Journal of Clinical Medicine (JCM) was opened from September 2019 to April 2020 [...]

Cited by 4 publications

References 33 publications

Reproduction of the Antitumor Effect of Cisplatin and Cetuximab Using a Three-dimensional Spheroid Model in Oral Cancer

Reproduction of the Antitumor Effect of Cisplatin and Cetuximab Using a Three-dimensional Spheroid Model in Oral Cancer

Expression of miR-31 in saliva-liquid biopsy in patients with oral squamous cell carcinoma

Oral squamous cell carcinoma

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