Organometallic hotspot engineering for ultrasensitive EC-SERS detection of pathogenic bacteria-derived DNAs

Lee, Soo Hyun; Lee, Won‐Chul; Koh, Eun Hye; Ansah, Iris Baffour; Yang, Jun‐Yeong; Mun, ChaeWon; Lee, Seunghun; Kim, Dongho; Jung, Ho Sang; Park, Sung‐Gyu

doi:10.1016/j.bios.2022.114325

Cited by 21 publications

(8 citation statements)

References 37 publications

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“…With a longer reaction, the plasmonic properties of SGBMNCs were substantially degraded. The signal enhancement was related to the coupling effect of interior hotspots with environmental hotspots [ 14 ]. The efficiency of the interior hotspots was related to the shell thickness as well as the skin depth, while that of the environmental hotspots (i.e., conventional hotspot regions between the AgNPs) was related to the gap distance.…”

Section: Resultsmentioning

confidence: 99%

“…Highly linear quantitativeness was observed, with a correlation coefficient ( R 2 ) of 0.95, 0.98, 0.91, and 0.96 for the peaks at 509, 1182, 1395, and 1620 cm –1 , respectively. The LOD of the MB-SGBMNC was evaluated using the equation [ 14 ]:

where S b is the standard deviation of a blank sample ( n = 3) and m is the slope of the calibration plot. Accordingly, the average LOD was determined to be 42 pM.…”

Section: Resultsmentioning

confidence: 99%

“…In the conventional development of SERS platforms, hotspot domains have been formed on surfaces or in the volumes of plasmonic nanostructures; thus, SERS signals are generated when analytes are placed in these restricted areas [ 10 , 11 , 12 ]. However, molecules with random diffusion can transfer and attach to any surface, including nonplasmonic or weak plasmonic regions, resulting in a degradation of the sensitivity and the limit-of-detection (LOD), especially at lower analyte concentrations [ 13 , 14 ]. Meanwhile, interior hotspots prepared by encapsulating analytes with metal layers have been introduced [ 14 , 15 , 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

“…However, molecules with random diffusion can transfer and attach to any surface, including nonplasmonic or weak plasmonic regions, resulting in a degradation of the sensitivity and the limit-of-detection (LOD), especially at lower analyte concentrations [ 13 , 14 ]. Meanwhile, interior hotspots prepared by encapsulating analytes with metal layers have been introduced [ 14 , 15 , 16 , 17 ]. Under this concept, dye molecules adsorbed onto substrate surfaces can produce fingerprint spectra simultaneously during redox processes.…”

Section: Introductionmentioning

confidence: 99%

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Interior Hotspot Engineering in Ag–Au Bimetallic Nanocomposites by In Situ Galvanic Replacement Reaction for Rapid and Sensitive Surface-Enhanced Raman Spectroscopy Detection

Ansah

Lee

Mun

et al. 2022

IJMS

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Engineering of interior hotspots provides a paradigm shift from traditional surface-enhanced Raman spectroscopy (SERS), in which the detection sensitivity depends on the positioning of adsorbed molecules. In the present work, we developed an Ag–Au bimetallic nanocomposite (SGBMNC) SERS platform with interior hotspots through facile chemical syntheses. Ag nanoparticles replaced by Au via the galvanic replacement reaction (GRR) provided hotspot regions inside the SGBMNC that remarkably enhanced the plasmonic activity compared to the conventional SERS platforms without the internal hotspots. The diffusion of analytes into the proposed interior hotspots during the GRR process enabled sensitive detections within 10 s. The SERS behaviors of the SGBMNC platform were investigated using methylene blue (MB) as a Raman probe dye. A quantitative study revealed excellent detection performance, with a limit of detection (LOD) of 42 pM for MB dye and a highly linear correlation between peak intensity and concentration (R2 ≥ 0.91). The SGBMNC platform also enabled the detection of toxic benzyl butyl phthalate with a sufficient LOD of 0.09 ppb (i.e., 280 pM). Therefore, we believe that the proposed methodology can be used for SERS assays of hazardous materials in practical fields.

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Section: Resultsmentioning

confidence: 99%

where S b is the standard deviation of a blank sample ( n = 3) and m is the slope of the calibration plot. Accordingly, the average LOD was determined to be 42 pM.…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Interior Hotspot Engineering in Ag–Au Bimetallic Nanocomposites by In Situ Galvanic Replacement Reaction for Rapid and Sensitive Surface-Enhanced Raman Spectroscopy Detection

Ansah

Lee

Mun

et al. 2022

IJMS

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show abstract

“…The label-free SERS profiles of bacteria offer signatures of molecular structures, cellular compositions, and physiological states, and can be applied to discriminate between bacterial species or to distinguish between live and dead bacteria [ 39 , 40 ]. For the label-based SERS methods to detect bacterial contamination, recognition elements, such as antibodies, aptamers, or small-molecule ligands, must be cross-linked to the SERS tags to improve the specificity for the targeted bacterial pathogen ( Table 4 ) [ 41 , 42 ].…”

Section: Sers Application In “From Farm To Table” Foodsmentioning

confidence: 99%

Innovative Application of SERS in Food Quality and Safety: A Brief Review of Recent Trends

Gao

Han

et al. 2022

Foods

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Innovative application of surface-enhanced Raman scattering (SERS) for rapid and nondestructive analyses has been gaining increasing attention for food safety and quality. SERS is based on inelastic scattering enhancement from molecules located near nanostructured metallic surfaces and has many advantages, including ultrasensitive detection and simple protocols. Current SERS-based quality analysis contains composition and structural information that can be used to establish an electronic file of the food samples for subsequent reference and traceability. SERS is a promising technique for the detection of chemical, biological, and harmful metal contaminants, as well as for food poisoning, and allergen identification using label-free or label-based methods, based on metals and semiconductors as substrates. Recognition elements, including immunosensors, aptasensors, or molecularly imprinted polymers, can be linked to SERS tags to specifically identify targeted contaminants and perform authenticity analysis. Herein, we highlight recent studies on SERS-based quality and safety analysis for different foods categories spanning the whole food chain, ‘from farm to table’ and processing, genetically modified food, and novel foods. Moreover, SERS detection is a potential tool that ensures food safety in an easy, rapid, reliable, and nondestructive manner during the COVID-19 pandemic.

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Galvanically Replaced Au/Ag Nanostructures as a SERS‐Active Substrate with Progressive Interior Hotspots

Shin,

Lee,

Ansah

et al. 2023

Advanced Sensor Research

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Interior hotspots in surface‐enhanced Raman spectroscopy (SERS) platforms have attracted intensive attention because they enable facile methodologies and exhibit excellent sensing behavior. Molecules surrounded by plasmonic materials exhibit dual functions as field‐confined regions and analytes, and their domain consistency eventually triggers the amplification of SERS signals. In this study, to conveniently realize interior hotspots, hollow regions such as voids and interstitials are strategically introduced via a galvanic reaction (GR) as a result of the difference in reduction potential between Au and Ag. The imbalanced stoichiometric ratio and diffusion fluxes induce the Kirkendall effect in conjunction with the GR between Au and Ag. SERS platforms with narrow and densified interior hotspots are optimized by controlling the reaction time. The activation of interior hotspots is confirmed using the finite‐difference time‐domain method, which indicates a theoretical enhancement factor of 1.07 × 107 based on a fourth‐power approximation. The spontaneous GR reaction enables sensing operation with high reproducibility (relative standard deviation of <10%). The proposed bimetallic platforms are used to trace methylene blue and rhodamine 6G dyes until their concentrations reach 50 nm. Therefore, the GR methodology for interior hotspot engineering demonstrates the potential for enabling the fabrication of SERS platforms with practical applications.

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Organometallic hotspot engineering for ultrasensitive EC-SERS detection of pathogenic bacteria-derived DNAs

Cited by 21 publications

References 37 publications

Interior Hotspot Engineering in Ag–Au Bimetallic Nanocomposites by In Situ Galvanic Replacement Reaction for Rapid and Sensitive Surface-Enhanced Raman Spectroscopy Detection

Interior Hotspot Engineering in Ag–Au Bimetallic Nanocomposites by In Situ Galvanic Replacement Reaction for Rapid and Sensitive Surface-Enhanced Raman Spectroscopy Detection

Innovative Application of SERS in Food Quality and Safety: A Brief Review of Recent Trends

Galvanically Replaced Au/Ag Nanostructures as a SERS‐Active Substrate with Progressive Interior Hotspots

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