Organometric parameters of the remaining kidney after removal of the contralateral in immature rats

Abstract

The processes of compensatory hypertrophy of the kidney to the period of puberty are insufficiently studied. Changes in renal parameters after contralateral removal are of scientific interest. The purpose of the study was to establish and compare changes in organometric parameters of the kidney that remained after nephrectomy in non-sexually adult male rats and in sexually mature male rats. Experimental study was performed on 84 non-adult white male rats weighing 53-71 grams, which were kept on a standard vivarium diet. All animals were divided into two groups (42 in each): the first - control, the second - the experimental group. In the control group, the abdominal cavity was opened under ketamine anesthesia, after wich the abdominal wall was sutured in layers. All animals of the experimental group performed surgical intervention - nephrectomy of the left kidney. Animals were withdrawn from the trial by intra-pleural administration of thiopental-sodium 50 mg/kg after 7, 14, 21, 30, 90 days after nephrectomy. Macroscopic evaluation and description of the kidneys of animals was performed after their removal. The statistical analysis of the obtained results was carried out using the “STATISTICA 5.5” program, using parametric and non-parametric methods for evaluating the obtained results. It was found that in the immature rats, the weight of animals in the experimental group (after nephrectomy) was statistically significantly lower in all terms of the observation compared to the control group, and the weight of the kidney of animals in the experimental group, on the contrary, was statistically significantly higher in all terms of observation. The length, width, thickness, volume of kidneys of animals of the experimental group, as well as the magnitude of increment of these parameters with each term of observation are determined. The greatest increase in organometric parameters of the kidney was observed after 30 days of the postoperative period (p<0.05). Subsequently, on the 60th and 90th day, the magnitude of the increase in organometric parameters of the kidney significantly decreased. The index of hypertrophy of the kidney remained unpaired by nephrectomy, which varied from 52.24% to 63.21%. The highest rates of hypertrophy were observed between the 21st and 30th days.