Organophosphorus pesticide quinalphos (Ekalux 25 E.C.) reduces sperm functional competence and decreases the fertilisation potential in Swiss albino mice

Kumari, Sandhya; Dcunha, Reyon; Sanghvi, Sahil Piyush; Nayak, Guruprasad; Kalthur, Sneha Guruprasad; Raut, Sushil; Mutalik, Srinivas; Siddiqui, Sazada; Alrumman, Sulaiman A.; Adiga, Satish Kumar; Kalthur, Guruprasad

doi:10.1111/and.14115

Cited by 10 publications

(4 citation statements)

References 62 publications

(114 reference statements)

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“…Increased anxiety in persons with Aβ plaques gave an immediate decrease in the cognitive routine. Animals that spent more time in the center area had lower anxiety levels than those that spent less time there . One of the behavioral evaluation tests, the raised plus maze test, resulted in a higher anxiety score.…”

Section: Discussionmentioning

confidence: 94%

“…Animals that spent more time in the center area had lower anxiety levels than those that spent less time there. 23 One of the behavioral evaluation tests, the raised plus maze test, resulted in a higher anxiety score. It was further demonstrated by a drop in entry counting and a decrease in time spared in an open arm, both of which imply anxiety levels.…”

Section: Discussionmentioning

confidence: 99%

“…Experimental procedures were carried out during 4 am to 8 pm. These albino mice were permitted free access to their food and water …”

Section: Experimental Animalsmentioning

confidence: 99%

“…These albino mice were permitted free access to their food and water. 23 Approval by the Ethical Committee. All the animals and protocols for the experimental design were implemented after the approval from the ethics committee of the Pharmacy Department, University of Malakand, Chakdara, Lower Dir, KPK.…”

Section: ■ Experimental Animalsmentioning

confidence: 99%

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Prospective Evaluation of an Amide-Based Zinc Scaffold as an Anti-Alzheimer Agent: In Vitro, In Vivo, and Computational Studies

et al. 2022

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Alzheimer’s disease is the most common progressive neurodegenerative mental disorder associated with loss of memory, decline in cognitive function, and dysfunction of language. The prominent pathogenic causes of this disease involve deposition of amyloid-β plaques, acetylcholine neurotransmitter deficiency, and accumulation of neurofibrillary tangles. There are multiple pathways that have been targeted to treat this disease. The inhibition of the intracellular cyclic AMP regulator phosphodiesterase IV causes the increase in CAMP levels that play an important role in the memory formation process. Organometallic chemistry works in a different way in treating pharmacological disorders. In the field of medicinal chemistry and pharmaceuticals, zinc-based amide carboxylates have been shown to be a preferred pharmacophore. The purpose of this research work was to investigate the potential of zinc amide carboxylates in inhibition of phosphodiesterase IV for the Alzheimer’s disease management. Swiss Albino mice under controlled conditions were divided into seven groups with 10 mice each. Group I was injected with carboxymethylcellulose (CMC) at 1 mL/100 g dose, group II was injected with Streptozotocin (STZ) at 3 mg/kg dose, group III was injected with Piracetam acting as a standard drug at 200 mg/kg dosage, while groups IV–VII were injected with a zinc scaffold at the dose regimen of 10, 20, 40, and 80 mg/kg through intraperitoneal injection. All groups except group I were injected with Streptozotocin on the first day and third day of treatment at the dose of 3 mg/kg through an intracerebroventricular route to induce Alzheimer’s disease. Afterward, respective treatment was continued for all groups for 23 days. In between the treatment regimen, groups were analyzed for memory and learning improvement through various behavioral tests such as open field, elevated plus maze, Morris water maze, and passive avoidance tests. At the end of the study, different biochemical markers in the brain were estimated like neurotransmitters (dopamine, serotonin and adrenaline), oxidative stress markers (superoxide dismutase, glutathione, and catalase), acetylcholinesterase (AchE), tau proteins, and amyloid-β levels. A PCR study was also performed. Results showed that the LD 50 of the zinc scaffold is greater than 2000 mg/kg. Research indicated that the zinc scaffold has the potential to improve the memory impairment and learning behavior in Alzheimer’s disease animal models in a dose-dependent manner. At the dose of 80 mg/kg, a maximum response was observed for the zinc scaffold. Maximum reduction in the acetylcholinesterase enzyme was observed at 80 mg/kg dose, which was further strengthened and verified by the PCR study. Oxidative stress was restored by the zinc scaffold due to the significant activation of the endogenous antioxidant enzymes. This research ended up with the conclusion that the zinc-based amide carboxylate scaffold has the potential to improve behavioral disturbances and vary the biochemica...

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

“…Experimental procedures were carried out during 4 am to 8 pm. These albino mice were permitted free access to their food and water …”

Section: Experimental Animalsmentioning

confidence: 99%

Section: ■ Experimental Animalsmentioning

confidence: 99%

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Prospective Evaluation of an Amide-Based Zinc Scaffold as an Anti-Alzheimer Agent: In Vitro, In Vivo, and Computational Studies

et al. 2022

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Enhanced cell survival in prepubertal testicular tissue cryopreserved with membrane lipids and antioxidants rich cryopreservation medium

Dcunha,

Aravind,

Bhaskar

et al. 2024

Cell Tissue Res

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The present study explores the advantages of enriching the freezing medium with membrane lipids and antioxidants in improving the outcome of prepubertal testicular tissue cryopreservation. For the study, testicular tissue from Swiss albino mice of prepubertal age group (2 weeks) was cryopreserved by slow freezing method either in control freezing medium (CFM; containing DMSO and FBS in DMEM/F12) or test freezing medium (TFM; containing soy lecithin, phosphatidylserine, phosphatidylethanolamine, cholesterol, vitamin C, sodium selenite, DMSO and FBS in DMEM/F12 medium) and stored in liquid nitrogen for at least one week. The tissues were thawed and enzymatically digested to assess viability, DNA damage, and oxidative stress in the testicular cells. The results indicate that TFM significantly mitigated freeze–thaw-induced cell death, DNA damage, and lipid peroxidation compared to tissue cryopreserved in CFM. Further, a decrease in Cyt C, Caspase-3, and an increase in Gpx4 mRNA transcripts were observed in tissues frozen with TFM. Spermatogonial germ cells (SGCs) collected from tissues frozen with TFM exhibited higher cell survival and superior DNA integrity compared to those frozen in CFM. Proteomic analysis revealed that SGCs experienced a lower degree of freeze–thaw-induced damage when cryopreserved in TFM, as evident from an increase in the level of proteins involved in mitigating the heat stress response, transcriptional and translational machinery. These results emphasize the beneficial role of membrane lipids and antioxidants in enhancing the cryosurvival of prepubertal testicular tissue offering a significant stride towards improving the clinical outcome of prepubertal testicular tissue cryopreservation.

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Biodegradation and metabolic pathway of quinalphos by Cunninghamella elegans ATCC36112

Zhang

Mei

et al. 2023

Biotechnol Lett

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Organophosphorus pesticide quinalphos (Ekalux 25 E.C.) reduces sperm functional competence and decreases the fertilisation potential in Swiss albino mice

Cited by 10 publications

References 62 publications

Prospective Evaluation of an Amide-Based Zinc Scaffold as an Anti-Alzheimer Agent: In Vitro, In Vivo, and Computational Studies

Prospective Evaluation of an Amide-Based Zinc Scaffold as an Anti-Alzheimer Agent: In Vitro, In Vivo, and Computational Studies

Enhanced cell survival in prepubertal testicular tissue cryopreserved with membrane lipids and antioxidants rich cryopreservation medium

Biodegradation and metabolic pathway of quinalphos by Cunninghamella elegans ATCC36112

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