Organoselenides from Nicotiana tabacum genetically modified to accumulate selenium

Matich, Adam J.; McKenzie, Marian J.; Brummell, David A.; Rowan, Daryl D.

doi:10.1016/j.phytochem.2009.06.001

Cited by 15 publications

(8 citation statements)

References 29 publications

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“…Se 120 caused necrotic lesions on the leaves and limited growth, which were signs of Se toxicity at this level. In nonaccumulator plants, stunting, necrotic lesions on the leaves, and decreased root growth have been mentioned as symptoms of Se toxicity (Matich et al, 2009). Plant growth was influenced by the applied concentrations of AsA and/or Se, especially the latter one, as it was indicated by the altered leaf characteristics.…”

Section: Leaf Characteristicsmentioning

confidence: 99%

The modified qualities of basil plants by selenium and/or ascorbic acid

Ardebili

Ardebili²,

Jalili³

et al. 2015

Turk J Bot

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IntroductionAscorbic acid (AsA) is one of the most critical low molecular weight antioxidants and has control over crucial processes, including the cell cycle and cell wall expansion (Noctor and Foyer, 1998). Ascorbic acid, a water soluble compound, is known as a major redox buffer, one of the most abundant antioxidants, and a required cofactor for several enzymes. Its multiple implications in various critical aspects of key physiological processes like cell division, growth, signal transduction, photosynthesis, and hormone biosynthesis are well documented (Gallie, 2013). Ascorbate is regarded as the first line of defense against detrimental effects of active oxygen species (AOS), and protects plant cells from various abiotic and biotic oxidative stressors (Suza et al., 2010). It has been stated that foliarly supplemented ascorbate may be an effective treatment for improving tolerance in stress exposed plants in arid and semi-arid regions (Dolatabadian et al., 2009). In addition, it has been proposed that various crucial compounds, including phenolics, carotenoids, tocopherols, chlorophyll, and ascorbic acid all possess a key role in the prevention of different diseases related to oxidative stress such as cardiovascular diseases, cancer, atherosclerosis, inflammation, neurodegenerative diseases, diabetes, and other chronic diseases (Šircelj et al., 2010).

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Section: Leaf Characteristicsmentioning

confidence: 99%

The modified qualities of basil plants by selenium and/or ascorbic acid

Ardebili

Ardebili²,

Jalili³

et al. 2015

Turk J Bot

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“…Firstly direct substitution of Se for S in the amino acids cysteine, SeMeCys and Met leads to the production of dimethylselenide (Me 2 Se), dimethyldiselenide (Me 2 Se 2 ) and dimethylthioselenide (MeSSeMe) in transgenic tobacco ( Nicotiana benthamiana ; Matich et al, 2009 ), Se-enhanced green onions ( Allium fistulosum ; Shah et al, 2007 ) and Brassicas (Matich et al, 2012 ; Ouerdane et al, 2013 ). Enzymatic degradation of MeSeCys or SeMet may also result in the production of 2-(methylseleno)acetaldehyde, or the methylselenides so produced may react further with unsaturated aldehydes (2-alkenals) resulting from lipid oxidation (Matich et al, 2009 ). Thirdly, enzymatic hydrolysis of methylselenoalkyl-glucosinolates leads to methylselenoalkylnitriles and methylselenoalkylisothiocyanates (Matich et al, 2012 , 2015 ).…”

Section: Instrumental Approaches For Detecting Measuring and Monitomentioning

confidence: 99%

“…However, selenoxides (e.g., dimethylselenoxide Me 2 SeO analogous to dimethylsulphoxide Me 2 SO) containing beta hydrogens (R 2 CHC(SeO ) R 2 ) and selenones (analogous to sulphones such as dimethylsulphone Me 2 SO 2 ) are thermally unstable above room temperatures and are not suitable for standard GC-MS analysis. For highly volatile selenides such as Me 2 Se and Me 2 Se 2 , headspace analysis using highly adsorbent graphite based SPME phases such as Carboxen TM or Carboxen TM -PDMS hybrid fibers (Matich et al, 2009 ) should be preferred. Further increases in sensitivity could be expected by the application of Stir Bar Sorptive Extraction SBSE (Twister) combined with cyrofocusing of volatiles onto the GC column.…”

Section: Instrumental Approaches For Detecting Measuring and Monitomentioning

confidence: 99%

Mechanisms of Selenium Enrichment and Measurement in Brassicaceous Vegetables, and Their Application to Human Health

Wiesner-Reinhold¹,

Schreiner²,

Baldermann³

et al. 2017

Front. Plant Sci.

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Selenium (Se) is an essential micronutrient for human health. Se deficiency affects hundreds of millions of people worldwide, particularly in developing countries, and there is increasing awareness that suboptimal supply of Se can also negatively affect human health. Selenium enters the diet primarily through the ingestion of plant and animal products. Although, plants are not dependent on Se they take it up from the soil through the sulphur (S) uptake and assimilation pathways. Therefore, geographic differences in the availability of soil Se and agricultural practices have a profound influence on the Se content of many foods, and there are increasing efforts to biofortify crop plants with Se. Plants from the Brassicales are of particular interest as they accumulate and synthesize Se into forms with additional health benefits, such as methylselenocysteine (MeSeCys). The Brassicaceae are also well-known to produce the glucosinolates; S-containing compounds with demonstrated human health value. Furthermore, the recent discovery of the selenoglucosinolates in the Brassicaceae raises questions regarding their potential bioefficacy. In this review we focus on Se uptake and metabolism in the Brassicaceae in the context of human health, particularly cancer prevention and immunity. We investigate the close relationship between Se and S metabolism in this plant family, with particular emphasis on the selenoglucosinolates, and consider the methodologies available for identifying and quantifying further novel Se-containing compounds in plants. Finally, we summarize the research of multiple groups investigating biofortification of the Brassicaceae and discuss which approaches might be most successful for supplying Se deficient populations in the future.

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“…Molecular cloning of SMTs was recently achieved in a number of higher plant species, including Astragalus bisulcatus 15 , Arabidopsis thaliana 16 , Oryza sativa 17 , Nicotiana tobacum 18 , Brassica oleracea 19 , and Camellia sinensis 20 . Until now, no SMT gene from T. wilfordii has been cloned.…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning and functional identification of sterol C24-methyltransferase gene from Tripterygium wilfordii

Guan

Zhao

et al. 2017

Acta Pharmaceutica Sinica B

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Sterol C24-methyltransferase (SMT) plays multiple important roles in plant growth and development. SMT1, which belongs to the family of transferases and transforms cycloartenol into 24-methylene cycloartenol, is involved in the biosynthesis of 24-methyl sterols. Here, we report the cloning and characterization of a cDNA encoding a sterol C24-methyltransferase from Tripterygium wilfordii (TwSMT1). TwSMT1 (GenBank access number KU885950) is a 1530 bp cDNA with a 1041 bp open reading frame predicted to encode a 346-amino acid, 38.62 kDa protein. The polypeptide encoded by the SMT1 cDNA was expressed and purified as a recombinant protein from Escherichia coli (E. coli) and showed SMT activity. The expression of TwSMT1 was highly up-regulated in T. wilfordii cell suspension cultures treated with methyl jasmonate (MeJA). Tissue expression pattern analysis showed higher expression in the phellem layer compared to the other four organs (leaf, stem, xylem and phloem), which is about ten times that of the lowest expression in leaf. The results are meaningful for the study of sterol biosynthesis of T. wilfordii and will further lay the foundations for the research in regulating both the content of other main compounds and growth and development of T. wilfordii.

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Organoselenides from Nicotiana tabacum genetically modified to accumulate selenium

Cited by 15 publications

References 29 publications

The modified qualities of basil plants by selenium and/or ascorbic acid

The modified qualities of basil plants by selenium and/or ascorbic acid

Mechanisms of Selenium Enrichment and Measurement in Brassicaceous Vegetables, and Their Application to Human Health

Molecular cloning and functional identification of sterol C24-methyltransferase gene from Tripterygium wilfordii

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