Organosolv pretreatment of lignocellulosic biomass for enzymatic hydrolysis

Zhao, Xuebing; Cheng, Ke-Ke; Liu, Dehua

doi:10.1007/s00253-009-1883-1

Cited by 1,021 publications

(708 citation statements)

References 66 publications

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“…These pretreatments include dilute acid (Lloyd and Wyman 2005;Saha et al 2005;Schell et al 2003), hot water (Liu and Wyman 2004;Ruiz et al 2013), ammonia fiber expansion (Hoover et al 2014;Lau et al 2008;Murnen et al 2007), steam explosion (Grous et al 1986;Kaar et al 1998), lime (Chang et al 1997;Kim and Holtzapple 2005), organic solvent (Zhang et al 2007;Zhao et al 2009b), and pyrolysis and mechanical disruption (Mosier et al 2005). In all these treatments, the substantial degradation of lignin is accompanied by considerable reduction in fermentable sugar content of the feedstock, resulting in a loss of 20-35% of the mass of lignocellulose (Galbe and Zacchi 2007;Lee et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Ionic liquid-tolerant microorganisms and microbial communities for lignocellulose conversion to bioproducts

Simmons

Singer

et al. 2016

Appl Microbiol Biotechnol

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Chemical and physical pretreatment of biomass is a critical step in the conversion of lignocellulose to biofuels and bioproducts. Ionic liquid (IL) pretreatment has attracted significant attention due to the unique ability of certain ILs to solubilize some or all components of the plant cell wall. However, these ILs not only inhibit enzyme activities, but also the growth and productivity of microorganisms used in downstream hydrolysis and fermentation processes.While pretreated biomass can be washed to remove residual IL and reduce inhibition, extensive washing is costly and not feasible in large-scale processes. IL tolerant microorganisms and microbial communities have been discovered from environmental samples and studies begun to elucidate mechanisms of IL tolerance. The discovery of IL tolerance in environmental microbial communities and individual microbes has lead to the proposal of molecular mechanisms of resistance. In this article, we review recent progress on discovering IL tolerant microorganisms, identifying metabolic pathways and mechanisms of tolerance, and engineering microorganisms for IL tolerance. Research in these areas will yield new approaches to overcome inhibition in lignocellulosic biomass bioconversion processes and increase opportunities for the use of ILs in biomass pretreatment.

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Section: Introductionmentioning

confidence: 99%

Ionic liquid-tolerant microorganisms and microbial communities for lignocellulose conversion to bioproducts

Simmons

Singer

et al. 2016

Appl Microbiol Biotechnol

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“…(4) Organosolv Process Organosolv process [19] achieves high lignin removal and minimum cellulose loss. Numerous organic or aqueous solvent mixtures can be utilized, including methanol, ethanol, acetone, ethylene glycol and tetrahydrofurfuryl alcohol, in order to solubilise lignin and enable the recovery of relatively pure lignin [20]. But, the high commercial prices of solvents hinder their industrial applications.…”

Section: A Physical Pretreatmentsmentioning

confidence: 99%

Biological Pretreatment of Lignocellulosic Substrates for Enhanced Delignification and Enzymatic Digestibility

2011

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Sheer enormity of lignocellulosics makes them potential feedstock for biofuel production but, their conversion into fermentable sugars is a major hurdle. They have to be pretreated physically, chemically, or biologically to be used by fermenting organisms for production of ethanol. Each lignocellulosic substrate is a complex mix of cellulose, hemicellulose and lignin, bound in a matrix. While cellulose and hemicellulose yield fermentable sugars, lignin is the most recalcitrant polymer, consisting of phenyl-propanoid units. Many microorganisms in nature are able to attack and degrade lignin, thus making access to cellulose easy. Such organisms are abundantly found in forest leaf litter/composts and especially include the wood rotting fungi, actinomycetes and bacteria. These microorganisms possess enzyme systems to attack, depolymerize and degrade the polymers in lignocellulosic substrates. Current pretreatment research is targeted towards developing processes which are mild, economical and environment friendly facilitating subsequent saccharification of cellulose and its fermentation to ethanol. Besides being the critical step, pretreatment is also cost intensive. Biological treatments with white rot fungi and Streptomyces have been studied for delignification of pulp, increasing digestibility of lignocellulosics for animal feed and for bioremediation of paper mill effluents. Such lignocellulolytic organisms can prove extremely useful in production of bioethanol when used for removal of lignin from lignocellulosic substrate and also for cellulase production. Our studies on treatment of hardwood and softwood residues with Streptomyces griseus isolated from leaf litter showed that it enhanced the mild alkaline solubilisation of lignins and also produced high levels of the cellulase complex when growing on wood substrates. Lignin loss (Klason lignin) observed was 10.5 and 23.5% in case of soft wood and hard wood, respectively. Thus, biological pretreatment process for lignocellulosic substrate using lignolytic organisms such as actinomycetes and white rot fungi can be developed for facilitating efficient enzymatic digestibility of cellulose.

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“…d ‐xylose, l ‐arabinose, d ‐glucose, d ‐mannose, d ‐galactose), furan derivatives, phenolic residues and other extractives, and was proposed to be used for fermentation and production of chemicals (e.g. xylitol, furfural) (Zhao et al ., 2009). Still, due to its complexity, the HF remains difficult to access.…”

Section: Introductionmentioning

confidence: 99%

Harnessing novel chromosomal integration loci to utilize an organosolv‐derived hemicellulose fraction for isobutanol production with engineered Corynebacterium glutamicum

Lange

Müller

Blombach

2017

Microbial Biotechnology

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SummaryA successful bioeconomy depends on the manifestation of biorefineries that entirely convert renewable resources to valuable products and energies. Here, the poorly exploited hemicellulose fraction (HF) from beech wood organosolv processing was applied for isobutanol production with Corynebacterium glutamicum. To enable growth of C. glutamicum on HF, we integrated genes required for d‐xylose and l‐arabinose metabolization into two of 16 systematically identified and novel chromosomal integration loci. Under aerobic conditions, this engineered strain CArXy reached growth rates up to 0.34 ± 0.02 h−1 on HF. Based on CArXy, we developed the isobutanol producer strain CIsArXy, which additionally (over)expresses genes of the native l‐valine biosynthetic and the heterologous Ehrlich pathway. CIsArXy produced 7.2 ± 0.2 mM (0.53 ± 0.02 g L−1) isobutanol on HF at a carbon molar yield of 0.31 ± 0.02 C‐mol isobutanol per C‐mol substrate (d‐xylose + l‐arabinose) in an anaerobic zero‐growth production process.

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Organosolv pretreatment of lignocellulosic biomass for enzymatic hydrolysis

Cited by 1,021 publications

References 66 publications

Ionic liquid-tolerant microorganisms and microbial communities for lignocellulose conversion to bioproducts

Ionic liquid-tolerant microorganisms and microbial communities for lignocellulose conversion to bioproducts

Biological Pretreatment of Lignocellulosic Substrates for Enhanced Delignification and Enzymatic Digestibility

Harnessing novel chromosomal integration loci to utilize an organosolv‐derived hemicellulose fraction for isobutanol production with engineered Corynebacterium glutamicum

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