Oricola cellulosilytica gen. nov., sp. nov., a cellulose-degrading bacterium of the family Phyllobacteriaceae isolated from surface seashore water, and emended descriptions of Mesorhizobium loti and Phyllobacterium myrsinacearum

Abstract

A Gram-stain negative, strictly aerobic, rod-shaped, non-spore-forming, motile cellulolytic bacterium, designated strain CC-AMH-0(T), was isolated from surface seashore water of Hualien, Taiwan and subjected to polyphasic taxonomy. Strain CC-AMH-0(T) exhibited enzymatic saccharification of cellulose and active growth particularly during log-phase under nutrient-limited conditions, whereas enhanced saccharification was found in the declining growth phase under copiotrophic conditions. The novel strain shared high pairwise 16S rRNA gene sequence similarities to Mesorhizobium loti USDA 3471(T) (96.2 %), Phyllobacterium myrsinacearum IAM 13584(T) (95.9 %), Hoeflea marina LMG 128(T) (94.0 %) and other Phyllobacteriaceae members. However, phylogenetic analyses based on 16S rRNA, atpD and recA gene sequences clearly distinguished strain CC-AMH-0(T) from other representatives of related genera. In addition, strain CC-AMH-0(T) was distinguished from the above mentioned species by significantly lacking phosphatidylcholine besides accommodating major amounts of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmonomethylethanolamine and sulfoquinovosyldiacylglycerol; moderate amounts of phosphatidylethanolamine and trace amounts of an unidentified phospholipid, an unidentified aminolipid and an unidentified phosphoglycolipid. Strain CC-AMH-0(T) possessed C18:1 ω7c and/or C18:1 ω6c (summed feature 8) as predominant fatty acids, 63.3 mol% DNA G+C content and ubiquinone-10 (Q-10) as the sole respiratory quinone. On the basis of polyphasic taxonomic evidences, strain CC-AMH-0(T) is proposed to represent a novel genus and species of the family Phyllobacteriaceae, for which the name Oricola cellulosilytica gen. nov., sp. nov. is proposed. The type strain of the type species is CC-AMH-0(T) (=JCM 19534(T) =BCRC 80694(T)). Emended descriptions of M. loti and P. myrsinacearum are also proposed.

Assessing the bacterial communities of sponges inhabiting the remote western Indian Ocean island of Mayotte

“…were observed in both sediment and L. paradoxa and are assigned to the order Rhizobiales (family Phyllobacteriaceae). OTU-21 is closely related to the novel taxon Oricola cellulosilytica, which was very recently described from surface seashore seawater in Taiwan (Hameed et al, 2015). However, the ecological relevance of many Phyllobacteriaceae representatives remains largely unknown.…”

Metagenomic and Metaproteomic Insights into Photoautotrophic and Heterotrophic Interactions in a Synechococcus Culture

“…Planctomycetes can exhibit diverse lifestyle strategies, including motility, unicellular stages, and aggregate formation with variable numbers of cells that are stacked with a fibrillar glycoproteic material known as the holdfast (40). Oricola cellulosilytica CC-AMH-0T is the only known member of the Interactions between Photoautotrophs and Heterotrophs ® genus and has been previously shown to degrade cellulose and become particularly active under nutrient-limited conditions (41). Balneola has also been frequently isolated from phytoplankton cultures and particularly from those of Emiliania huxley and Cochlodinium polykrikoides (42,43).…”

Thalassocola ureilytica gen. nov., sp. nov., of the family Phyllobacteriaceae isolated from seawater

“…The family Phyllobacteriaceae (Knösel, 1984) has been classified under the order Rhizobiales , class Alphaproteobacteria . At the time of writing, the family accommodates 12 genera, Phyllobacterium (Knösel, 1984; Mergaert et al , 2002), Aminobacter (Urakami et al , 1992), Mesorhizobium (Jarvis et al , 1997), Aquamicrobium (Bambauer et al , 1998; Fritsche et al , 1999; Kämpfer et al , 2009), Pseudaminobacter (Kämpfer et al , 1999), Nitratireductor (Labbé et al , 2004), Hoeflea (Peix et al , 2005), Chelativorans (Doronina et al , 2010), ‘ Aliihoeflea ’ (Roh et al , 2008), Pseudahrensia (Jung et al , 2012), Thermovum (Yabe et al , 2012) and Oricola (Hameed et al , 2015). The type strains of the type species of the genera Pseudahrensia , Nitratireductor , Hoeflea , ‘ Aliihoeflea ’ and Oricola were isolated from marine environments, whereas those of the type species of other genera in the family were from non-marine sources.…”

“…Cells have been characterized to harbour predominant amounts of C 18 : 1 ω7 c /C 18 : 1 ω6 c fatty acids, ubiquinone-10 (Q-10) as the respiratory quinone and a high (>50.0 mol%) DNA G+C content (Yabe et al , 2012). Diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylmonomethylethanolamine (PME), phosphatidyldimethylethanolamine (PDE), hydroxyphosphatidylethanolamine (OH-PME), sulfoquinovosyldiacylglycerol (SQDG) and phosphatidylinositol (PI) have been heterogeneously encountered as polar lipids within the Phyllobacteriaceae (Kämpfer et al , 1999; Peix et al , 2005; Doronina et al , 2010; Jung et al , 2012; Yabe et al , 2012; Hameed et al , 2015).…”

“… * DPG, diphosphatidylglycerol; PG, phosphatidylglycerol; PE, phosphatidylethanolamine; PME, phosphatidylmonomethylethanolamine; PC, phosphatidylcholine; SQDG, sulfoquinovosyldiacylglycerol; PGL, phosphoglycolipid; AL, unidentified aminolipid; PL, unidentified phospholipid; GL, unidentified glycolipid; L, unidentified lipid. † Data from Hameed et al (2015). ‡ Data from Jung et al (2012). § Data for strains 2–6 are from Peix et al (2005), Labbé et al (2004), Hameed et al (2015), Jarvis et al (1997) and Mergaert et al (2002), respectively. …”