Oridonin, A natural diterpenoid, protected NGF-differentiated PC12 cells against MPP+- and kainic acid-induced injury

Lin, Kuan Ho; Li, Chien Yu; Hsu, Yu-Chin; Tsai, Chang Hai; Tsai, Fuu‐Jen; Tang, Chih Hsin; Yang, Jai Sing; Wang, Zhi Hong; Yin, Mei

doi:10.1016/j.fct.2019.110765

Cited by 29 publications

(18 citation statements)

References 31 publications

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“…To the best of our knowledge, this is the rst report to demonstrate an anti-depressive function of Ori, although this drug has recently been recommended as a potential drug for the treatment of tumors [50][51][52] and neurodegenerative diseases such as AD and PD [26,27,53]. In this study, both prophylactic and therapeutic treatments with Ori (5 mg/kg and 10mg/kg) effectively improved depressive-like behaviors induced by CUMS, with the effects of the former being more pronounced than the latter.…”

Section: Discussionmentioning

confidence: 99%

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Oridonin relieves depressive-like behaviors by inhibiting neuroinflammation and autophagy impairment in rats subjected to chronic unpredictable mild stress

Liang¹,

Wang²,

Wang³

et al. 2021

Preprint

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Background: Major depressive disorder (MDD) is a common, chronic, and severe life-threatening disorder with increasing prevalence. Although neuroinflammation is associated with the etiology of depression, the mechanistic interplay between depression, neuroinflammation, and autophagy is yet to be demonstrated. This study investigated the effect of Oridonin on CUMS-induced neuroinflammation, depression, and autophagy impairment. Methods: Male 4-week-old Sprague-Dawley rats were subjected to chronic unpredictable mild stress (CUMS) for 4 and 6 weeks respectively, some of which were injected with Oridonin, fluoxetine (FLX) or their combination at different durations of CUMS. After CUMS procedure, sucrose preference test and Forced swim test were used to evaluate depressive-like behaviors. Concomitant neuroinflammation changes and autophagy impairments were examined in the hippocampus.Reults: In this study, we found that chronic stress resulted in depressive-like behaviors and caused neuroinflammation and autophagy impairment. In particular, CUMS treatment significantly increased the levels of cytokines (IL-1β, IL-18 and Caspase-1), reduced autophagy-related protein levels (Beclin-1, p62, Atg5 and LC3B), caused microglia cells activation. Oridonin treatment can prevent and reverse the depressive-like behavior induced by CUMS in prophylactic and therapeutic administration, but there is ceiling effect in the treatment process. Furthermore, 10mg/kg dose of Ori has a stronger and longer lasting antidepressant effect than the 10mg/kg dose of fluoxetine. And the antidepressant effect of Ori in combination with fluoxetine was greater than that of high-dose fluoxetine alone. In addition, Ori treatment significantly normalized autophagy-related protein levels, and reduced levels of cytokines via blocking the interaction between NLRP3 and NEK7. Further, we investigated the role of autophagy in LPS-activated BV2 cells and levels of cytokines. Similarly, Oridonin treatment abolished and reversed levels of cytokines and autophagy-related protein levels.Conclusions: All these results supported our hypothesis that Ori possesses potent anti-depressive action, which might be mediated via inhibition of neuroinflammation and autophagy impairment through blocking the interaction between NLRP3 and NEK7.

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Section: Discussionmentioning

confidence: 99%

“…Oridonin (Ori), a diterpenoid isolated from Rabdosia rubescens, has multiple biological properties, especially anti-in ammatory and neuroregulatory activities [24,25]. It can attenuate behavioural de cits in Alzheimer's disease (AD) [26][27][28], which means that Ori has the potential application against neurodegenerative disorders.…”

Section: Introductionmentioning

confidence: 99%

Oridonin relieves depressive-like behaviors by inhibiting neuroinflammation and autophagy impairment in rats subjected to chronic unpredictable mild stress

Liang¹,

Wang²,

Wang³

et al. 2021

Preprint

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“…Next, the formazan crystals were dissolved in DMSO following the removal of the medium. The formazan product was analyzed spectrophotometrically at a wavelength of 490 nm (24). This analysis was performed in triplicate.…”

Section: Methodsmentioning

confidence: 99%

Next‑generation sequencing analysis reveals that MTH‑3, a novel curcuminoid derivative, suppresses the invasion of MDA‑MB‑231 triple‑negative breast adenocarcinoma cells

et al. 2021

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Triple-negative breast cancer (TNBC) behaves aggressively in the invasive and metastatic states. Our research group recently developed a novel curcumin derivative, (1E,3Z,6E)-3-hydroxy-5-oxohepta-1,3,6-triene-1,7-diyl)bis(2methoxy-4,1-phenylene)bis(3-hydroxy-2-hydroxymethyl)-2methyl propanoate (MTH-3), and previous studies showed that MTH-3 inhibits TNBC proliferation and induces apoptosis in vitro and in vivo with a superior bioavailability and absorption than curcumin. In the present study, the effects of MTH-3 on TNBC cell invasion were examined using various assays and gelatin zymography, and western blot analysis. Treatment with MTH-3 inhibited MDA-MB-231 cell invasion and migration, as shown by Transwell assay, 3D spheroid invasion assay, and wound healing assay. The results of the gelatin zymography experiments revealed that MTH-3 decreased matrix metalloproteinase-9 activity. The potential signaling pathways were revealed by next-generation sequencing analysis, antibody microarray analysis and western blot analysis. In conclusion, the results of the present study show that, MTH-3 inhibited tumor cell invasion through the MAPK/ERK/AKT signaling pathway and cell cycle regulatory cascade, providing significant information about the potential molecular mechanisms of the effects of MTH-3 on TNBC.

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“…CNS comprises the brain and the spinal cord, while the PNS encompasses the rest of the system other than the brain and spinal cord [2]. Patients that are involved in major traumas often suffer from peripheral nerve damages due to the extent of the impact, and such damages usually include nerve compression, nerve lesions, or tear, or even ischemic injuries [3]. Other reasons that can cause peripheral nerve damage include surgical procedures and other co-morbidities.…”

Section: Introductionmentioning

confidence: 99%

Additive Manufacturing of Nerve Decellularized Extracellular Matrix-Contained Polyurethane Conduits for Peripheral Nerve Regeneration

Chen

et al. 2019

Polymers

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The nervous system is the part of our body that plays critical roles in the coordination of actions and sensory information as well as communication between different body parts through electrical signal transmissions. Current studies have shown that patients are likely to experience a functional loss if they have to go through a nerve repair for >15 mm lesion. The ideal treatment methodology is autologous nerve transplant, but numerous problems lie in this treatment method, such as lack of harvesting sites. Therefore, researchers are attempting to fabricate alternatives for nerve regeneration, and nerve conduit is one of the potential alternatives for nerve regeneration. In this study, we fabricated polyurethane/polydopamine/extracellular matrix (PU/PDA/ECM) nerve conduits using digital light processing (DLP) technology and assessed for its physical properties, biodegradability, cytocompatibility, neural related growth factor, and proteins secretion and expression and its potential in allowing cellular adhesion and proliferation. It was reported that PU/PDA/ECM nerve conduits were more hydrophilic and allowed enhanced cellular adhesion, proliferation, expression, and secretion of neural-related proteins (collagen I and laminin) and also enhanced expression of neurogenic proteins, such as nestin and microtubule-associated protein 2 (MAP2). In addition, PU/PDA/ECM nerve conduits were reported to be non-cytotoxic, had sustained biodegradability, and had similar physical characteristics as PU conduits. Therefore, we believed that PU/PDA/ECM nerve conduits could be a potential candidate for future nerve-related research or clinical applications.

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Oridonin, A natural diterpenoid, protected NGF-differentiated PC12 cells against MPP+- and kainic acid-induced injury

Cited by 29 publications

References 31 publications

Oridonin relieves depressive-like behaviors by inhibiting neuroinflammation and autophagy impairment in rats subjected to chronic unpredictable mild stress

Oridonin relieves depressive-like behaviors by inhibiting neuroinflammation and autophagy impairment in rats subjected to chronic unpredictable mild stress

Next‑generation sequencing analysis reveals that MTH‑3, a novel curcuminoid derivative, suppresses the invasion of MDA‑MB‑231 triple‑negative breast adenocarcinoma cells

Additive Manufacturing of Nerve Decellularized Extracellular Matrix-Contained Polyurethane Conduits for Peripheral Nerve Regeneration

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