Orientation and food search behaviour of a deep sea lobster in turbulent versus laminar odour plumes

Major, Robert; Jeffs, Andrew

doi:10.1186/s10152-017-0489-8

Cited by 12 publications

(7 citation statements)

References 53 publications

(83 reference statements)

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“…The diets previously determined microscopically are at a poor taxonomic resolution, and included crustaceans, fish, annelids and bivalves ( Choi et al, 2008 ; Sahlmann, Chan & Chan, 2011 ; Wahle et al, 2012 ). Metanephrops challengeri are thought to be benthic foragers and scavengers, relying heavily on chemosensory detection of potential food items ( Major & Jeffs, 2017 ). They are likely to be scavengers of fish remains whether it is from trawl debris, sunken carcasses or faeces, as well as foraging for smaller dietary items such as sea pens.…”

Section: Discussionmentioning

confidence: 99%

Preliminary analysis of New Zealand scampi (Metanephrops challengeri) diet using metabarcoding

Reis

Laroche

Jeffs

et al. 2018

PeerJ

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Deep sea lobsters are highly valued for seafood and provide the basis of important commercial fisheries in many parts of the world. Despite their economic significance, relatively little is known about their natural diets. Microscopic analyses of foregut content in some species have suffered from low taxonomic resolution, with many of the dietary items difficult to reliably identify as their tissue is easily digested. DNA metabarcoding has the potential to provide greater taxonomic resolution of the diet of the New Zealand scampi (Metanephrops challengeri) through the identification of gut contents, but a number of methodological concerns need to be overcome first to ensure optimum DNA metabarcoding results. In this study, a range of methodological parameters were tested to determine the optimum protocols for DNA metabarcoding, and provide a first view of M. challengeri diet. Several PCR protocols were tested, using two universal primer pairs targeting the 18S rRNA and COI genes, on DNA extracted from both frozen and ethanol preserved samples for both foregut and hindgut digesta. The selection of appropriate DNA polymerases, buffers and methods for reducing PCR inhibitors (including the use of BSA) were found to be critical. Amplification from frozen or ethanol preserved gut contents appeared similarly dependable. The COI gene was found to be more effective than 18S rRNA gene for identifying large eukaryotic taxa from the digesta; however, it was less successfully amplified. The 18S rRNA gene was more easily amplified, but identified mostly smaller marine organisms such as plankton and parasites. This preliminary analysis of the diet of M. challengeri identified a range of species (13,541 reads identified as diet), which included the ghost shark (Hydrolagus novaezealandiae), silver warehou (Seriolella punctata), tall sea pen (Funiculina quadrangularis) and the salp (Ihlea racovitzai), suggesting that they have a varied diet, with a high reliance on scavenging a diverse range of pelagic and benthic species from the seafloor.

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Section: Discussionmentioning

confidence: 99%

Preliminary analysis of New Zealand scampi (Metanephrops challengeri) diet using metabarcoding

Reis

Laroche

Jeffs

et al. 2018

PeerJ

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show abstract

“…The computational fluid dynamics solver software, ANSYS-CFX ® 2018, was used to create a 0.5 m wide, 0.15 m tall, and 3.5 m long computational flume containing a turbulent flow, with a pipe inlet that releases odorant 3 cm above the bed 0.5 m downstream (Figure 2). The size of the flume is comparable to that used in behavioral experiments [18,28,45]. Water enters through the upstream edge of the flume at 0.1 m•s −1 and exits the outlet 3.5 m downstream.…”

Section: Computational Fluid Dynamics Simulationmentioning

confidence: 99%

Interpreting the Spatial-Temporal Structure of Turbulent Chemical Plumes Utilized in Odor Tracking by Lobsters

Leathers

Michaelis

Reidenbach

2020

Fluids

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Olfactory systems in animals play a major role in finding food and mates, avoiding predators, and communication. Chemical tracking in odorant plumes has typically been considered a spatial information problem where individuals navigate towards higher concentration. Recent research involving chemosensory neurons in the spiny lobster, Panulirus argus, show they possess rhythmically active or ‘bursting’ olfactory receptor neurons that respond to the intermittency in the odor signal. This suggests a possible, previously unexplored olfactory search strategy that enables lobsters to utilize the temporal variability within a turbulent plume to track the source. This study utilized computational fluid dynamics to simulate the turbulent dispersal of odorants and assess a number of search strategies thought to aid lobsters. These strategies include quantification of concentration magnitude using chemosensory antennules and leg chemosensors, simultaneous sampling of water velocities using antennule mechanosensors, and utilization of antennules to quantify intermittency of the odorant plume. Results show that lobsters can utilize intermittency in the odorant signal to track an odorant plume faster and with greater success in finding the source than utilizing concentration alone. However, the additional use of lobster leg chemosensors reduced search time compared to both antennule intermittency and concentration strategies alone by providing spatially separated odorant sensors along the body.

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“…Manuscript to be reviewed Metanephrops challengeri are thought to be benthic foragers and scavengers, relying heavily on chemosensory detection of potential food items (Major & Jeffs 2017). They are likely to be scavengers of fish remains whether it is from trawl debris, sunken carcases or faeces, as well as foraging for smaller dietary items such as sea pens.…”

Section: Preliminary Assessment Of M Challengeri Dietmentioning

confidence: 99%

Peer Review #2 of "Preliminary analysis of New Zealand scampi (Metanephrops challengeri) diet using metabarcoding (v0.1)"

2018

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Deep sea lobsters are highly valued for seafood and provide the basis of important commercial fisheries in many parts of the world. Despite their economic significance, relatively little is known about their natural diets. Microscopic analyses of foregut content in some species have suffered from low taxonomic resolution, with many of the dietary items difficult to reliably identify as their tissue is easily digested. DNA metabarcoding has the potential to provide greater taxonomic resolution of the diet of the New Zealand scampi (Metanephrops challengeri) through the identification of gut contents, but a number of methodological concerns need to be overcome first to ensure optimum DNA metabarcoding results. In this study, a range of methodological parameters were tested to determine the optimum protocols for DNA metabarcoding, and provide a first view of M. challengeri diet. Several PCR protocols were tested, using two universal primer pairs targeting the 18S rRNA and COI genes, on DNA extracted from both frozen and ethanol preserved samples for both foregut and hindgut digesta. The selection of appropriate DNA polymerases, buffers and methods for reducing PCR inhibitors (including the use of BSA) were found to be critical. Amplification from frozen or ethanol preserved gut contents appeared similarly dependable. The COI gene was found to be more effective than 18S rRNA gene for identifying large eukaryotic taxa from the digesta, however, it was less successfully amplified. The 18S rRNA gene was more easily amplified, but identified mostly smaller marine organisms such as plankton and parasites. This preliminary analysis of the diet of M. challengeri identified a range of species (13,541 reads identified as diet), which included the ghost shark (Hydrolagus novaezealandiae), silver warehou (Seriolella punctate), tall sea pen (Funiculina quadrangularis) and the salp (Ihlea racovitza), suggesting that they have

show abstract

Orientation and food search behaviour of a deep sea lobster in turbulent versus laminar odour plumes

Cited by 12 publications

References 53 publications

Preliminary analysis of New Zealand scampi (Metanephrops challengeri) diet using metabarcoding

Preliminary analysis of New Zealand scampi (Metanephrops challengeri) diet using metabarcoding

Interpreting the Spatial-Temporal Structure of Turbulent Chemical Plumes Utilized in Odor Tracking by Lobsters

Peer Review #2 of "Preliminary analysis of New Zealand scampi (Metanephrops challengeri) diet using metabarcoding (v0.1)"

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