Orientation and motion of spin-labels in rabbit small intestinal brush border vesicle membranes

Häuser, H.; Gains, Nigel; Semenza, Giorgio; Spiess, Martin

doi:10.1021/bi00265a036

Cited by 37 publications

(36 citation statements)

References 54 publications

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“…This difference (P1) was more pronounced at 4°C (P1 = 0.102) than at 180C (P1 = 0.063) or 37°C (P1 = 0.052). Therefore the flow-cytometry technique has allowed us to confirm results obtained by Hauser et al (1982) by e.s.r. spectroscopy.…”

Section: Resultssupporting

confidence: 52%

Flow cytometry is a new method for the characterization of intestinal plasma membrane

Gorvel¹,

Mawas²,

Maroux³

et al. 1984

Biochemical Journal

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The highly differentiated plasma membrane of rabbit enterocytes constitutes an interesting model for membrane studies. Flow cytometry allows combined measurements of the size of membrane vesicles by light-scatter and fluorescence polarization at a single-particle level. The degree of fluorescence polarization of 1,6-diphenylhexa-1,3,5-triene was determined at 4, 18 and 37 degrees C in the brush-border and basolateral plasma membranes. The fluorescence polarization was considerably higher in brush-border than in basolateral membranes. After incubation with dimethyl sulphoxide, the membrane fluidity decreased in both types of membranes. Moreover, a time-effect study of dimethyl sulphoxide showed changes in fluorescence polarization. Only in brush-border membrane a temporary fluid phase was observed. The different properties of the two membrane domains in relation to the lipid-protein dynamics of enterocytes are discussed.

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Section: Resultssupporting

confidence: 52%

Flow cytometry is a new method for the characterization of intestinal plasma membrane

Gorvel¹,

Mawas²,

Maroux³

et al. 1984

Biochemical Journal

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“…Heating the fully hydrated lipid from 0 "C gave a broad endothermic transition between about 15 and 40 OC with peaks at about 20 and 30 "C. It is noteworthy that for aqueous dispersions of the pure lipid the heating ( Figure 5C) and the cooling curves ( Figure 5D) are both composite, conthat of a dipalmitoylphosphatidylcholine bilayer in the gel state at about 30 OC (Hauser et al, 1979). The S33 value is also comparable to those measured in other bacterial membranes (Esser & Lanyi, 1973;Plachy et al, 1974;Chignell & Chignell, 1975;Hauser et al, 1982). We can conclude that the packing of the lipids in this membrane is characterized by a high order or high motional anisotropy.…”

Section: Discussionsupporting

confidence: 74%

Characterization of membrane lipids of a general fatty acid auxotrophic bacterium by electron spin resonance spectroscopy and differential scanning calorimetry

Häuser¹,

Hazlewood²,

Dawson³

1985

Biochemistry

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Lipids in the plasma membrane of the general fatty acid auxotroph Butyrivibrio S2 pack as a bilayer that is characterized by a high order and high motional anisotropy and a low membrane fluidity compared to mammalian plasma membranes. Lipid packing as determined by the electron spin resonance (ESR) order parameter and membrane fluidity as measured by ESR correlation times are, however, comparable to those of other bacterial membranes. Membranes of the organism grown with saturated fatty acids of well-defined hydrocarbon chain length undergo a broad reversible endothermic phase transition, the peak temperature of which is well below the growth temperature; the end-point temperature of this thermal transition approximately coincides with the minimum temperature supporting significant growth of the organism. The lipid phase transition is also reflected in the temperature dependence of various ESR parameters, whereby the transition temperature thus derived is higher than the peak temperature of the endothermic transition but still lower than the growth temperature. ESR and calorimetry evidence taken together suggest that the endothermic transition is a gel to liquid-crystal transition and that, at the growth temperature, the plasma membrane of Butyrivibrio S2 is in the liquid-crystalline state. Similar values were measured for the order parameter of cell membranes of Butyrivibrio S2 regardless of whether the organism was grown on myristic, palmitic, or stearic acid. Butyrivibrio S2 has a mechanism enabling it to maintain membrane packing and fluidity at a fairly constant level.(ABSTRACT TRUNCATED AT 250 WORDS)

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“…A comparison of the order parameter values obtained at 370C for 5-doxylstearic acid and 12-doxylstearic acid in a variety of biological membranes was presented by Hauser no W0. et al (23). A summary of that data plus the S values obtained in the present work are given in Table I.…”

Section: Resultssupporting

confidence: 53%

Lipid phase of transverse tubule membranes from skeletal muscle. An electron paramagnetic resonance study

Hidalgo¹

1985

Biophysical Journal

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The lipid phase of transverse tubule membrane was probed with a variety of fatty acid spin labels. The motion of the probe increased as the distance between the spin label and polar head group increased, in agreement with results reported in other membranes. The value of the order parameter at 37 degrees C for a fatty acid spin label containing the label attached to its fifth carbon atom was closer to values reported for bacterial membranes than to the lower values reported for other mammalian membranes. Order parameters for spin labels containing the label nearer to the center of the bilayer were closer to the values reported in other mammalian membranes than to values reported for bacterial membranes. These results indicate that the lipid segments in the vicinity of the polar head group, and less so those near the center of the bilayer, are motionally more restricted in transverse tubules than in other mammalian membranes. In particular, the lipid phase of the transverse tubule membrane is less fluid than that of the sarcoplasmic reticulum membrane. A possible role of the high cholesterol content of transverse tubules in generating the lower fluidity of its lipid phase is discussed.

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Orientation and motion of spin-labels in rabbit small intestinal brush border vesicle membranes

Cited by 37 publications

References 54 publications

Flow cytometry is a new method for the characterization of intestinal plasma membrane

Flow cytometry is a new method for the characterization of intestinal plasma membrane

Characterization of membrane lipids of a general fatty acid auxotrophic bacterium by electron spin resonance spectroscopy and differential scanning calorimetry

Lipid phase of transverse tubule membranes from skeletal muscle. An electron paramagnetic resonance study

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