Orientation-independent rapid pulsatile flow measurement using dual-angle Doppler OCT

Peterson, Lindsy M.; Gu, Shi; Jenkins, Michael W.; Rollins, Andrew M.

doi:10.1364/boe.5.000499

Cited by 18 publications

(16 citation statements)

References 51 publications

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“…Embryos were imaged using a custom‐built spectral‐domain OCT system with a quasi‐telecentric scanner (Hu and Rollins, , ; Ma et al., ; Peterson et al., ). The OCT light source is a superluminescent diode centered at 1,310 nm with a full width at half‐maximum bandwidth of 75 nm.…”

Section: Methodsmentioning

confidence: 99%

Supplementation with the Methyl Donor Betaine Prevents Congenital Defects Induced by Prenatal Alcohol Exposure

Karunamuni

Sheehan

Doughman

et al. 2017

Alcoholism Clin & Exp Res

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Background Despite decades of public education about dire consequences of prenatal alcohol exposure, drinking alcohol during pregnancy remains prevalent. As high as 40% of live-born infants exposed to alcohol during gestation and diagnosed with Fetal Alcohol Syndrome have congenital heart defects that can be life-threatening. In animal models, the methyl donor betaine, found in foods such as wheat bran, quinoa, beets and spinach, ameliorated neurobehavioral deficits associated with prenatal alcohol exposure (PAE) but effects on heart development are unknown. Methods Previously we modeled a binge drinking episode during the first trimester in avian embryos. Here we investigated whether betaine could prevent adverse effects of alcohol on heart development. Embryos exposed to ethanol with and without an optimal dose of betaine (5 μM) were analyzed at late developmental stages. Cardiac morphology parameters were rapidly analyzed and quantified using optical coherence tomography. DNA methylation at early stages was detected by immunofluorescent staining for 5-methylcytosine in sections of embryos treated with ethanol or co-treated with betaine. Results Compared to ethanol-exposed embryos, betaine-supplemented embryos had higher late-stage survival rates and fewer gross head and body defects than seen after alcohol exposure alone. Betaine also reduced the incidence of late-stage cardiac defects such as absent vessels, abnormal atrio-ventricular (AV) valves, and hypertrophic ventricles. Furthermore, betaine co-treatment brought measurements of great vessel diameters, interventricular septum (IVS) thickness, and AV leaflet volumes in betaine-supplemented embryos close to control values. Early-stage 5-methycytosine staining revealed that DNA methylation levels were reduced by ethanol exposure and normalized by co-administration with betaine. Conclusions This is the first study demonstrating efficacy of the methyl donor betaine in alleviating cardiac defects associated with PAE. These findings highlight the therapeutic potential of low-concentration betaine doses in mitigating PAE induced birth defects and has implications for prenatal nutrition policies, especially for women who may not be responsive to folate supplementation.

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Section: Methodsmentioning

confidence: 99%

Supplementation with the Methyl Donor Betaine Prevents Congenital Defects Induced by Prenatal Alcohol Exposure

Karunamuni

Sheehan

Doughman

et al. 2017

Alcoholism Clin & Exp Res

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“…Embryos were imaged using a custom‐built Fourier domain OCT system with a quasi‐telecentric scanner (Hu and Rollins, ; Ma et al, ; Peterson et al, ). The OCT light source is a superluminescent diode centered at 1,310 nm with a full‐width at half‐maximum bandwidth of 75 nm.…”

Section: Methodsmentioning

confidence: 99%

Using optical coherence tomography to rapidly phenotype and quantify congenital heart defects associated with prenatal alcohol exposure

Karunamuni

Doughman

et al. 2015

Developmental Dynamics

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Background The most commonly used method to analyze congenital heart defects involves serial sectioning and histology. However, this is often a time-consuming process where the quantification of cardiac defects can be difficult due to problems with accurate section registration. Here we demonstrate the advantages of using optical coherence tomography, a comparatively new and rising technology, to phenotype avian embryo hearts in a model of Fetal Alcohol Syndrome where a binge-like quantity of alcohol/ethanol was introduced at gastrulation. Results The rapid, consistent imaging protocols allowed for the immediate identification of cardiac anomalies, including ventricular septal defects and misaligned/missing vessels. Interventricular septum thicknesses and vessel diameters for three of the five outflow arteries were also significantly reduced. Outflow and atrio-ventricular valves were segmented using image processing software and had significantly reduced volumes compared to controls. This is the first study to our knowledge that has 3-D reconstructed the late-stage cardiac valves in precise detail in order to examine their morphology and dimensions. Conclusion We believe therefore that OCT, with its ability to rapidly image and quantify tiny embryonic structures in high resolution, will serve as an excellent and cost-effective preliminary screening tool for developmental biologists working with a variety of experimental/disease models.

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“…For example, 1.0 variability of Doppler angle can result in flow error greater than 20% when the Doppler angle is at 85 [32]. However, Phase wrapping confines its measureable range and hence restricts its applications.…”

Section: Discussionmentioning

confidence: 99%

“…In this case, the absolute flow calculation is highly dependent on the accuracy of Doppler angle. For example, 1.0 variability of Doppler angle can result in flow error greater than 20% when the Doppler angle is at 85 [32]. Therefore, Phase wrapping is inevitable under many conditions of DOCT based flow measurement.…”

Section: Discussionmentioning

confidence: 99%

Phase unwrapping for Doppler spectral domain optical coherence tomography flow measurement

Zhao

Liu

et al. 2019

Journal of Biophotonics

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Doppler optical coherence tomography (OCT) offers additional flow velocity information, which extends the application of OCT. Phase wrapping is the inherent problem that limits measureable range of Doppler OCT. We propose a phase unwrapping method which is suitable for correcting phase in Doppler OCT images. Points (pixels) in flow region are divided into groups according to the radial distance. Points in the same group are supposed to have close velocity. Phase unwrapping algorithm begins at the boundary layer group and is performed sequentially toward the center. Using the proposed criterion, points in a group are separated into two categories, signal points and noise points. Wrapping rounds are determined for signal points phase unwrapping. Mean value of the corrected signal points replaces the noise points for noise reduction. The method is validated with capillary tube flow phantom and in vivo blood flow. K E Y W O R D SDoppler, flow measurement, medical and biological imaging, optical coherence tomography, phase unwrapping

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Orientation-independent rapid pulsatile flow measurement using dual-angle Doppler OCT

Cited by 18 publications

References 51 publications

Supplementation with the Methyl Donor Betaine Prevents Congenital Defects Induced by Prenatal Alcohol Exposure

Supplementation with the Methyl Donor Betaine Prevents Congenital Defects Induced by Prenatal Alcohol Exposure

Using optical coherence tomography to rapidly phenotype and quantify congenital heart defects associated with prenatal alcohol exposure

Phase unwrapping for Doppler spectral domain optical coherence tomography flow measurement

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