Origanum essential oils reduce the level of melanin in B16-F1 melanocytes

Khoury, Rindala El; Michael‐Jubeli, Rime; Bakar, Joudi; Dakroub, Hani; Rizk, Toufic; Baillet‐Guffroy, Arlette; Lteif, Roger; Tfayli, Ali

doi:10.1684/ejd.2019.3677

Cited by 12 publications

(14 citation statements)

References 32 publications

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“…Forskolin and α-MSH were identified as potent cAMP activators which trigger melanogenesis in vitro [ 28 ]; in this study, we induced melanin production through a combination of α-MSH and forskolin and determined the inhibitory effect of CFEO. We found that CFEO treatment significantly blocked melanin production in B16 cells, which is well correlated with other observations that essential oils from Cinnamomum cassia, Melaleuca quinquenervia, Alpinia zerumbet , Alpinia nantoensis, Eucalyptus camaldulensis, Origanum syriacum, and Origanum ehrenbergii showed strong melanin inhibition in a similar condition to in vitro experiments [ 9 , 10 , 11 , 12 , 13 ]. In addition, essential oils have the capability to inhibit melanogenesis through dual inhibitory mechanisms, including direct inhibition of tyrosinase enzyme activity and downregulating the melanin biosynthesis pathway through modulating cellular signaling cascades [ 14 , 29 ].…”

Section: Discussionsupporting

confidence: 90%

“…Accumulating scientific evidence proved that essential oils can be used to treat various skin disorders, including acne, pre-mature aging, hyperpigmentation, as well as protecting skin from UV radiation. It has been reported that Origanum ( O. syriacum and O. ehrenbergii ) essential oils [ 9 ], leaf essential oils of Alpinia zerumbet [ 10 ], Melaleuca quinquenervia [ 11 ], leaf and rhizome essential oils of Alpinia nantoensis [ 12 ], flower essential oils of Eucalyptus camaldulensis [ 13 ], stem bark essential oils of Cinnamomum cassia [ 14 ] exhibited strong anti-melanogenic activity in vitro.…”

Section: Introductionmentioning

confidence: 99%

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Anti-Melanogenic Activity of Calocedrus formosana Wood Essential Oil and Its Chemical Composition Analysis

Hsiao

Kumar

Lee

et al. 2021

Plants

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Calocedrus formosana (Cupressaceae) is one of the five precious woods of Taiwan. In this study, we investigated the anti-melanogenic activity of C. formosana wood essential oil (CFEO) and its bioactive components in vitro. Initially, CFEO exhibited strong mushroom tyrosinase activity in the cell-free mushroom tyrosinase assay system with an IC50 value of 2.72 µg/mL. Next, treatment with CFEO significantly as well as dose-dependently reduced a combination of a-melanocyte-stimulating hormone and forskolin (a-MSH-FSK)-induced melanin synthesis in B16-F10 cells. Indeed, 80 mg/mL CFEO completely inhibited melanin production, which is similar to that of control cells. Further studies revealed that treatment with CFEO significantly inhibited melanogenesis regulatory proteins, including TRP-1, TRP-2, and MITF, whereas tyrosinase was unaffected by either a-MSH-FSK or CFEO. In addition, the composition of the CFEO was characterized. The major components of CFEO were α-terpineol (23.47%), shonanic acid (10.45%), terpinen-4-ol (12.23%), thymol (5.3%), piperitone (3.44%), berbenone (2.81%), thujic acid (1.65%), and chaminic acid (0.13%). Among them, shonanic acid (1), thujic acid (2), and chaminic acid (3) were uncommon constitutes in essential oils, which could be the index compounds of CFEO, and the structure of these compounds were confirmed by spectral analysis. Furthermore, we found that thymol is an active ingredient responsible for CFEO’s anti-melanogenic activity. Based on these results, we suggest that CFEO or thymol could be a potential candidate for the development of skin whitening products for cosmetic purposes.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Anti-Melanogenic Activity of Calocedrus formosana Wood Essential Oil and Its Chemical Composition Analysis

Hsiao

Kumar

Lee

et al. 2021

Plants

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“…The results showed that the application of the essential oil reduced the production of melanin by 15% at 40 µg/mL in those cells. In parallel, the melanogenic activity of pure carvacrol, the main component of the O. syriacum essential oil, was shown to significantly decrease melanin levels in those cells, implicating carvacrol in the process of melanogenesis [79]. In an effort to investigate the mechanism involved, the effects of the O. syriacum essential oil and pure carvacrol were tested on tyrosinase, which is the first enzyme involved in the process of synthesis of melanin and is the rate-limiting step.…”

Section: Antimelanogenic Activitymentioning

confidence: 99%

“…This is in agreement with an earlier study, as O. syriacum essential oil at 40 µg/mL and carvacrol at 2.5 µg/mL were able to inhibit the activity of tyrosinase in tubo by 80% and 56%, respectively [35]. The proposed mechanism of action of the O. syriacum extract involves a competitive inhibition model whereby carvacrol binds to tyrosinase, preventing the binding of its usual substrate tyrosine in a mechanism similar to that involving the commonly used skin-lightening agent hydroquinone in the treatment of hyperpigmentation [76,79] as seen in Figure 6. vacrol in the process of melanogenesis [79].…”

Section: Antimelanogenic Activitymentioning

confidence: 99%

Origanum syriacum Phytochemistry and Pharmacological Properties: A Comprehensive Review

et al. 2022

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Herbal medicine has been gaining special interest as an alternative choice of treatment for several diseases, being generally accessible, cost-effective and safe, with fewer side-effects compared to chemically synthesized medicines. Over 25% of drugs worldwide are derived from plants, and surveys have shown that, when available, herbal medicine is the preferred choice of treatment. Origanum syriacum (Lamiaceae) is a widely used medicinal plant in the Middle East, both as a home and a folk remedy, and in the food and beverage industry. Origanum syriacum contains numerous phytochemical compounds, including flavonoids, phenols, essential oils, and many others. Because of its bioactive compounds, O. syriacum possesses antioxidant, antimicrobial, and antiparasitic capacities. In addition, it can be beneficial in the treatment of various diseases such as cancer, neurodegenerative disorders, and peptic ulcers. In this review, the chemical compositions of different types of extracts and essential oils from this herb will first be specified. Then, the pharmacological uses of these extracts and essential oils in various contexts and diseases will be discussed, putting emphasis on their efficacy and safety. Finally, the cellular and molecular mechanisms of O. syriacum phytochemicals in disease treatment will be described as a basis for further investigation into the plant’s pharmacological role.

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“…Other EOs showing antimelanogenic properties, in terms of reduction of melanin synthesis, intracellular tyrosinase activity and MITF expression, when tested in B16-F10 cells, include those extracted from Vitex negundo [88], Origanum syriacum and Origanum ehrenbergii and their main component, carvacrol [59], Artemisia argyi [28], Chrysanthemum boreale Makino [31], two varieties of Alpinia Zerumbet EOs, shima and tairin oils [24], Achillea millefolium and its component lynalyl acetate blocking melanin production through the regulation of the JNK and ERK signaling pathways [23], Mentha aquatica (lime mint oil) and one of its main compounds, β-caryophyllene, [221], Vitex Trifolia and its main component abietatriene [89].…”

Section: Antimelanogenic Activitymentioning

confidence: 99%

Essential Oils and Their Main Chemical Components: The Past 20 Years of Preclinical Studies in Melanoma

Martile

Garzoli

Ragno

et al. 2020

Cancers

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The last two decades have seen the development of effective therapies, which have saved the lives of a large number of melanoma patients. However, therapeutic options are still limited for patients without BRAF mutations or in relapse from current treatments, and severe side effects often occur during therapy. Thus, additional insights to improve treatment efficacy with the aim to decrease the likelihood of chemoresistance, as well as reducing side effects of current therapies, are required. Natural products offer great opportunities for the discovery of antineoplastic drugs, and still represent a useful source of novel molecules. Among them, essential oils, representing the volatile fraction of aromatic plants, are always being actively investigated by several research groups and show promising biological activities for their use as complementary or alternative medicine for several diseases, including cancer. In this review, we focused on studies reporting the mechanism through which essential oils exert antitumor action in preclinical wild type or mutant BRAF melanoma models. We also discussed the latest use of essential oils in improving cancer patients’ quality of life. As evidenced by the many studies listed in this review, through their effect on apoptosis and tumor progression-associated properties, essential oils can therefore be considered as potential natural pharmaceutical resources for cancer management.

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Origanum essential oils reduce the level of melanin in B16-F1 melanocytes

Cited by 12 publications

References 32 publications

Anti-Melanogenic Activity of Calocedrus formosana Wood Essential Oil and Its Chemical Composition Analysis

Anti-Melanogenic Activity of Calocedrus formosana Wood Essential Oil and Its Chemical Composition Analysis

Origanum syriacum Phytochemistry and Pharmacological Properties: A Comprehensive Review

Essential Oils and Their Main Chemical Components: The Past 20 Years of Preclinical Studies in Melanoma

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