Origanum majorana essential oil improves the rat’s sexual behavior and testicular oxidative damage induced by imidacloprid via modulating the steroidogenesis pathways

Mehanna, Sally; Issa, Marwa Y.; Hassan, Neven H.; Hussien, Ahmed; Ibrahim, Marwa A.; Hassanen, Eman I.

doi:10.1016/j.jsps.2022.06.016

Cited by 20 publications

(8 citation statements)

References 47 publications

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“…[58] GSH is thought to be the main scavenger of the excess free radicals created by oxidative stress. [59] Our result agreed with Mehanna et al, [60] who found that oxidative stress lowers the level of GSH due to its consumption during oxidation.…”

Section: Notesupporting

confidence: 92%

Novel insights on the probable mechanism associated with histamine oral model‐inducing neuropathological and behavioral toxicity in rats

Mohamed,

Hassanen,

Mansour

et al. 2024

J Biochem & Molecular Tox

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Histamine (HIS) is an important chemical mediator that causes vasodilation and contributes to anaphylactic reactions. Recently, HIS is an understudied neurotransmitter in the central nervous system, and its potential role in neuroinflammation and neurodegeneration is a critical area of research. So, the study's goal is to investigate the consequences of repeated oral intake of HIS on the rat's brain and explore the mechanistic way of its neurotoxicity. Thirty male rats were divided into three groups (n = 10). The following treatments were administered orally to all rats every day for 14 days. Group (1) was given distilled water, whereas groups (2 & 3) were given HIS at dosage levels 250 and 500 mg/kg body weight (BWT), respectively. Brain tissue samples were collected at 7‐ and 14‐days from the beginning of the experiment. Our results revealed that continuous oral administration of HIS at both doses for 14 days significantly reduced the BWT and induced severe neurobehavioral changes, including depression, dullness, lethargy, tremors, abnormal walking, and loss of spatial learning and memory in rats. In all HIS receiving groups, HPLC data showed a considerable raise in the HIS contents of the brain. Additionally, the daily consumption of HIS causes oxidative stress that is dose‐ and time‐dependent which is characterized by elevation of malondialdehyde levels along with reduction of catalase activity and reduced glutathione levels. The neuropathological lesions were commonly observed in the cerebrum, striatum, and cerebellum and confirmed by the immunohistochemistry staining that demonstrating moderate to strong caspase‐3 and inducible nitric oxide synthase expressions in all HIS receiving groups, mainly those receiving 500 mg/kg HIS. NF‐κB, TNF‐α, and IL‐1β gene levels were also upregulated at 7‐ and 14‐days in all HIS groups, particularly in those getting 500 mg/kg. We concluded that ROS‐induced apoptosis and inflammation was the essential mechanism involved in HIS‐mediated neurobehavioral toxicity and histopathology.

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Section: Notesupporting

confidence: 92%

Novel insights on the probable mechanism associated with histamine oral model‐inducing neuropathological and behavioral toxicity in rats

Mohamed,

Hassanen,

Mansour

et al. 2024

J Biochem & Molecular Tox

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“…The reduction in serum testosterone levels caused by AlCl 3 could be due to atrophy of Leydig cells (Pandey & Jain, 2017 ). Furthermore, these negative effects of AlCl 3 on testosterone production could be explained in light of our data regarding gene analysis that illustrated marked reduction of the testicular mRNA expression levels of CYP11A1, HSD-3b, and StAR, all of which are required for steroidogenesis (Manna et al, 2009 ; Mehanna et al, 2022 ). In this regard, AlCl 3 decreased the number of Leydig cells while suppressing the mRNA expression of 3beta-hydroxysteroid dehydrogenase (3-HSD), 17-HSD, steroidogenic acute regulatory protein (STAR), and cholesterol side-chain cleavage enzyme (P450scc) (Mohammad et al, 2015 ).…”

Section: Discussionmentioning

confidence: 81%

Repro-protective activity of amygdalin and spirulina platensis in niosomes and conventional forms against aluminum chloride–induced testicular challenge in adult rats: role of CYP11A1, StAR, and HSD-3B expressions

Abo El-Ela,

Gamal,

El-Banna

et al. 2023

Naunyn-Schmiedeberg's Arch Pharmacol

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The male reproductive system is negatively influenced by Al exposure. Al represented a considerable hazard to men’s reproduction capabilities. Amygdalin (AMG) and spirulina platensis (SP) have been considered to have a strong antioxidant and repro-protective activity; also, targeted drug delivery systems called niosomes improve the distribution of water-soluble medications like amygdalin and spirulina. Current study targeted to determine the effectiveness of AMG and SP against negative reproductive impact resulted by aluminum chloride (AlCl3) toxicity. Sixty adult male albino rats were separated into 6 groups, including the control group, which received distilled water; AlCl3 group, which received AlCl3; AMG+AlCl3 group, which received AlCl3+AMG; AMGLN+AlCl3 group, which received AlCl3+amygdalin-loaded niosomes; SP+AlCl3 group, which received AlCl3+SP; and SPLN+AlCl3 group, which received AlCl3+spirulina-loaded niosomes. All treatments were orally gavaged daily for 5 weeks, and rats were weighed weekly. At the termination of the experiment, some males (three from each group) were used for fertility traits via mating thirty virgin rat females (in a ratio of 1:2 and 2:3 male:female, respectively) followed by recording of birth weights and litter size (number of pups per each female) at birth to assess males’ reproductive capability. Other males were euthanized for collection of serum, epididymal semen samples, and tissue samples for biochemical, sperm evaluation, gene expression, and histopathological measurements. There are a considerable number of negative impacts of AlCl3 on male fertility clarified by declined serum testosterone levels; an increased oxidative stress (MDA, TAC); deteriorated semen quality; down-regulation of CYP11A1, StAR, and HSD-3b gene expressions; and testicular tissue degenerative changes. In addition, litter size (number of pups per each female) and birth weights of pups obtained from mated females were affected. AMG and SP treatments, either in niosomal or conventional form, alleviated the AlCl3 negative effects by reducing oxidative stress; increasing testosterone levels; improving semen quality; upregulating of CYP11A1, StAR, and HSD-3b gene expressions; and reducing degenerative changes of testicular tissue. Besides, negative reproductive effect was diminished as observed by changes in the litter size (number of pups per each female) and birth weights of pups obtained from mated females. AMG and SP treatments (either in niosomal or conventional form), ameliorated the AlCl3 negative effects as they possess powerful antioxidant activity, as well as they have the ability to improve the reproductive activity of affected males. Graphical abstract

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Section: Resultsmentioning

confidence: 88%

“…The volatile constituents in the essential oil of O. majorana grown in Egypt were detected with relative ratios as recorded corresponding to their order of elution on Rtx-5MS fused bonded columns in a previous study. 34 This led to the identification of a total of 55 components. The most abundant volatile metabolites representative of OME were linalool (17.76%), γ-terpinen (14.26%), terpinen-4-ol (13.54%), α-terpinene (8.21%), cis -sabinene hydrate (5.89%), sabinene (5.62%), α-terpineol (4.74%), linalyl acetate (3.64%), terpinolene (3.32%), and limonene (3.25%) all belonging to the oxygenated and hydrocarbon monoterpenoid group.…”

Section: Resultsmentioning

confidence: 99%

Potential Mechanisms of Imidacloprid-Induced Neurotoxicity in Adult Rats with Attempts on Protection Using Origanum majorana L. Oil/Extract: In Vivo and In Silico Studies

et al. 2023

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Imidacloprid (IMI) insecticide is rapidly metabolized in mammals and contributes to neurotoxicity via the blocking of nicotinic acetylcholine receptors, as in insects. Origanum majorana retains its great antioxidant potential in both fresh and dry forms. No data is available on the neuroprotective effect of this plant in laboratory animals. In this context, aerial parts of O. majorana were used to prepare the essential oil (OMO) and methanol extract (OME). The potential neuroprotective impact of both OMO and OME against IMI-induced neurotoxicity in rats was explored. Forty-two rats were divided into 6 groups, with 7 rats in each one. Rats were daily administered the oral treatments: normal saline, OMO, OME, IMI, IMI + OMO, and IMI + OME. Our results revealed the identification of 55 components in O. majorana essential oil, most belonging to the oxygenated and hydrocarbon monoterpenoid group. Moreover, 37 constituents were identified in the methanol extract, mostly phenolics. The potent neurotoxic effect of IMI on rats was confirmed by neurobehavioral and neuropathological alterations and a reduction of both acetylcholine esterase (AchE) activity and dopamine (DA), serotonin (5HT), and γ-aminobutyric acid (GABA) levels in the brain. Exposure of rats to IMI elevates the malondialdehyde (MDA) levels and reduces the antioxidant capacity. IMI could upregulate the transcription levels of nuclear factor-κB (NF-κB), interleukin-1 β (IL-1β), and tumor necrosis factor (TNF-α) genes and express strong caspase-3 and inducible nitric oxide synthase (iNOS) immunostaining in most examined brain areas. On the other hand, rats coadministered OMO or OME with IMI showed a marked improvement in all of the studied toxicological parameters. In conclusion, cotreatment of O. majorana extracts with IMI can protect against IMI neurotoxicity via their potent antioxidant, anti-inflammatory, and anti-apoptotic effects. Thus, we recommend a daily intake of O. majorana to protect against insecticide’s oxidative stress-mediated neuroinflammatory stress and apoptosis. The molecular docking study of linalool, rosmarinic acid, γ-terpene, and terpene-4-ol justify the observed normalization of the elevated iNOS and TNF-α levels induced after exposure to IMI.

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Origanum majorana essential oil improves the rat’s sexual behavior and testicular oxidative damage induced by imidacloprid via modulating the steroidogenesis pathways

Cited by 20 publications

References 47 publications

Novel insights on the probable mechanism associated with histamine oral model‐inducing neuropathological and behavioral toxicity in rats

Novel insights on the probable mechanism associated with histamine oral model‐inducing neuropathological and behavioral toxicity in rats

Repro-protective activity of amygdalin and spirulina platensis in niosomes and conventional forms against aluminum chloride–induced testicular challenge in adult rats: role of CYP11A1, StAR, and HSD-3B expressions

Potential Mechanisms of Imidacloprid-Induced Neurotoxicity in Adult Rats with Attempts on Protection Using Origanum majorana L. Oil/Extract: In Vivo and In Silico Studies

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