Origin and Fate of Hematopoietic Precursors in the Early Mouse Embryo

Bertrand, Julien; Manaia, Alexandra; Celst, Jeanne Van; Cumano, Ana; Godin, Isabelle

doi:10.1007/978-0-387-33535-3_9

Cited by 2 publications

(1 citation statement)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Yet, when the early Sp is cultured in toto for a few days, it now produces hematopoietic precursors, which harbour a multilineage differentiation potential as well as LTR capacity [3,4]. As the Sp are explanted before the establishment of vascular connection with the extra-embryonic vascular network, which occurs at the 4–5 somite-stage [5,6], this observation shows that the intra-embryonic site is capable of autonomously generating HSC. In contrast, the progeny of similarly treated YS lacks both multipotentiality (they are unable to generate lymphoid cells) and LTR activity (they only provide short term erythro-myeloid reconstitution of irradiated recipients) [5,6].…”

Section: Introductionmentioning

confidence: 99%

Gene transfer to pre-hematopoietic and committed hematopoietic precursors in the early mouse Yolk Sac: a comparative study between in situ electroporation and retroviral transduction

et al. 2007

Self Cite

View full text Add to dashboard Cite

Background: Hematopoietic development in vertebrate embryos results from the sequential contribution of two pools of precursors independently generated. While intra-embryonic precursors harbour the features of hematopoietic stem cells (HSC), precursors formed earlier in the yolk sac (YS) display limited differentiation and self-renewal potentials. The mechanisms leading to the generation of the precursors in both sites are still largely unknown, as are the molecular basis underlying their different potential. A possible approach to assess the role of candidate genes is to transfer or modulate their expression/activity in both sites. We thus designed and compared transduction protocols to target either native extra-embryonic precursors, or hematopoietic precursors.

show abstract

Section: Introductionmentioning

confidence: 99%

Gene transfer to pre-hematopoietic and committed hematopoietic precursors in the early mouse Yolk Sac: a comparative study between in situ electroporation and retroviral transduction

et al. 2007

Self Cite

View full text Add to dashboard Cite

show abstract

Ontogeny of the Hematopoietic System

Cumano

Godin

2007

Annu. Rev. Immunol.

Self Cite

361

286

View full text Add to dashboard Cite

Blood cells are constantly produced in the bone marrow (BM) of adult mammals. This constant turnover ultimately depends on a rare population of progenitors that displays self-renewal and multilineage differentiation potential, the hematopoietic stem cells (HSCs). It is generally accepted that HSCs are generated during embryonic development and sequentially colonize the fetal liver, the spleen, and finally the BM. Here we discuss the experimental evidence that argues for the extrinsic origin of HSCs and the potential locations where HSC generation might occur. The identification of the cellular components playing a role in the generation process, in these precise locations, will be important in understanding the molecular mechanisms involved in HSC production from undifferentiated mesoderm.

show abstract

Origin and Fate of Hematopoietic Precursors in the Early Mouse Embryo

Cited by 2 publications

References 67 publications

Gene transfer to pre-hematopoietic and committed hematopoietic precursors in the early mouse Yolk Sac: a comparative study between in situ electroporation and retroviral transduction

Gene transfer to pre-hematopoietic and committed hematopoietic precursors in the early mouse Yolk Sac: a comparative study between in situ electroporation and retroviral transduction

Ontogeny of the Hematopoietic System

Contact Info

Product

Resources

About