Origin and levels of circulating microparticles in normal pregnancy: A longitudinal observation in healthy women

Radu, Claudia; Campello, Elena; Spiezia, Luca; Dhima, Sonila; Visentin, Silvia; Gavasso, S; Woodhams, Barry; Cosmi, Erich; Simioni, Paolo

doi:10.3109/00365513.2015.1052551

Cited by 17 publications

(18 citation statements)

References 37 publications

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“…d Signals of fetal maturity, such as surfactant protein A production by fetal lungs and stretch of the amniotic membranes, trigger inflammation, leading to the common pathway of parturition apoptotic bodies-are released by feto-placental tissues, potentially stemming from cytotrophoblasts, syncytiotrophoblasts, and placental mesenchymal stem cells. Concentrations of EVs increase linearly as pregnancy progresses, suggesting their role as pacemakers of pregnancy [116,137,158,160]. These EVs play a role in intercellular communication by serving as vehicles for transfer of membrane and cytosolic fetal proteins, lipids, and micro (mi)RNA [108].…”

Section: Immune Programming By Circulating Fetal Materialsmentioning

confidence: 99%

Multiomic immune clockworks of pregnancy

et al. 2020

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Preterm birth is the leading cause of mortality in children under the age of five worldwide. Despite major efforts, we still lack the ability to accurately predict and effectively prevent preterm birth. While multiple factors contribute to preterm labor, dysregulations of immunological adaptations required for the maintenance of a healthy pregnancy is at its pathophysiological core. Consequently, a precise understanding of these chronologically paced immune adaptations and of the biological pacemakers that synchronize the pregnancy "immune clock" is a critical first step towards identifying deviations that are hallmarks of peterm birth. Here, we will review key elements of the fetal, placental, and maternal pacemakers that program the immune clock of pregnancy. We will then emphasize multiomic studies that enable a more integrated view of pregnancy-related immune adaptations. Such multiomic assessments can strengthen the biological plausibility of immunological findings and increase the power of biological signatures predictive of preterm birth

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Section: Immune Programming By Circulating Fetal Materialsmentioning

confidence: 99%

Multiomic immune clockworks of pregnancy

et al. 2020

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“…Several studies have evaluated the concentrations and origins of circulating EVs in the maternal system and elucidated their ability to interact with the immune cells and regulate them [28,57,71,82]. Remarkably, pregnancy is characterized by higher levels of circulating EVs than non-pregnant state and they progressively increase with gestational age, achieving highest concentration in the third trimester [65,71,79]. During early stages of pregnancy, EVs derived from endometrium, embryo, and trophoblast cells can influence the maternal immune response, whereas towards the advanced stages of gestation, the EVs released by the placenta, mainly STB-derived EVs, are the key players (reviewed in [94]).…”

Section: Evs In Pregnancy and Their Effects On Maternal Immune Systemmentioning

confidence: 99%

Extracellular vesicles and their immunomodulatory functions in pregnancy

Nair

Salomón

2018

Semin Immunopathol

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Extracellular vesicles (EVs) are membrane-bound vesicles released into the extracellular space by almost all types of cells. EVs can cross the physiological barriers, and a variety of biological fluids are enriched in them. EVs are a heterogeneous population of vesicles, including exosomes, microvesicles, and apoptotic bodies. The different subpopulations of vesicles can be differentiated by size and origin, in which exosomes (~100 nm and from endocytic origin) are the most studied so far. EVs have essential roles in cell-to-cell communication and are critical modulators of immune response under normal and pathological conditions. Pregnancy is a unique situation of immune-modulation in which the maternal immune system protects the fetus from allogenic rejection and maintains the immunosurveillance. The placenta is a vital organ that performs a multitude of functions to support the pregnancy. The EVs derived from the human placenta have crucial roles in regulating the maternal immune response for successful pregnancy outcome. Placenta-derived vesicles perform a myriad of functions like suppression of immune reaction to the developing fetus and establishment and maintenance of a systemic inflammatory response to combat infectious intruders. A fine-tuning of these mechanisms is quintessential for successful completion of pregnancy and healthy outcome for mother and fetus. Dysregulation in the mechanisms mentioned above can lead to several pregnancy disorders. In this review, we summarize the current literature regarding the critical roles played by the EVs in immunomodulation during pregnancy with particular attention to the placenta-derived exosomes.

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“…The MP gate was established on the Cytomics FC500 (Beckman Coulter, Miami Florida) instrument by preliminary standardization experiments using a blend of sizecalibrated fluorescent polystyrene beads with sizes ranging from 0.1 to 3 lm (Megamix-Plus FSC 0.1 lm, 0.3 lm, 0.5 lm, and 0.9 lm and Megamix 0.5, 0.9, and 3.0 lm, both by BioCytex, Marseille, France), as previously described (33)(34)(35). In particular, the upper limit of the MP gate was established just above the size distribution of the 0.9 lm beads in a forward scatter (FS) and side scatter (SS) setting (log scale).…”

Section: Mp Flow Cytometric Analysismentioning

confidence: 99%

Activated Platelet‐Derived and Leukocyte‐Derived Circulating Microparticles and the Risk of Thrombosis in Heparin‐Induced Thrombocytopenia: A Role for PF4‐Bearing Microparticles?

Campello

Radu

Duner

et al. 2017

Cytometry Part B Clinical

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We showed for the first time the presence of circulating PF4-bearing MPs derived from activated platelets in patients with HIT; activated leukocyte/TF + MPs are associated with an increased thrombotic risk. Our findings confirm that HIT antibodies complexes may determine a TF-driven prothrombotic state through the activation of platelets and leukocytes. © 2017 International Clinical Cytometry Society.

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Origin and levels of circulating microparticles in normal pregnancy: A longitudinal observation in healthy women

Cited by 17 publications

References 37 publications

Multiomic immune clockworks of pregnancy

Multiomic immune clockworks of pregnancy

Extracellular vesicles and their immunomodulatory functions in pregnancy

Activated Platelet‐Derived and Leukocyte‐Derived Circulating Microparticles and the Risk of Thrombosis in Heparin‐Induced Thrombocytopenia: A Role for PF4‐Bearing Microparticles?

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