Origin and phylogenetic analysis of Tibetan Mastiff based on the mitochondrial DNA sequence

Li, Qifa; Liu, Zhenshan; Li, Yinxia; Zhao, Xingbo; Dong, Liyan; Pan, Zengxiang; Sun, Yuanrong; Li, Ning; Xu, Yinxue; Xie, Zhihua

doi:10.1016/s1673-8527(08)60049-1

Cited by 21 publications

(25 citation statements)

References 18 publications

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“…The abscissa is the proportion of distance and the vertical coordinate is the base transition and transversion. species form, which was consistent with the results reported by Luo (2003), Li et al (2008), and Li et al (2011). …”

Section: Resultssupporting

confidence: 93%

“…A total of 23 haplotypes were defined in the COI gene sequence of the Tibetan Mastiff and 76 domestic dog breeds (Table 1), and the haplotype percentage was 21.50%. The polymorphism was lower than that based on the mitochondrial displacement loop (D-loop; especially hypervariable region 1; Vilà et al, 1997;Savolainen et al, 2002;Luo, 2003), but higher than that based on the mitochondrial Cytb gene (Li et al, 2008). Vilà et al (1997) found 27 variable sites and identified 26 haplotypes in the 261-bp D-loop region with a 10.34% polymorphism loci percentage and an 18.57% haplotype percentage.…”

Section: Resultsmentioning

confidence: 73%

“…Luo (2003) found 113 variable loci and identified 184 haplotypes in the 597-bp D-loop with an 18.93% polymorphism loci percentage and a 15.11% haplotype percentage. Li et al (2008) found 88 variable loci in a 2525 bp mitochondrial sequence, with a 3.39% polymorphism loci percentage. Therefore, the COI gene, as the moderate variant mitochondrial DNA sequence, applied to the phylogenetic analysis among domestic dogs.…”

Section: Resultsmentioning

confidence: 98%

“…However, large profits can be made from the sale of Tibetan Mastiffs, and the outflow of Tibetan Mastiffs has become a serious problem since the 1980s. Therefore, the purebred Tibetan Mastiff is at the edge of extinction (Li et al, 2008). Being the only large breed dog in China and one of the most idioplasmatic resources worldwide, the Tibetan Mastiff must be protected and rapidly researched.…”

Section: Introductionmentioning

confidence: 99%

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The origin of the Tibetan Mastiff and species identification of Canis based on mitochondrial cytochrome c oxidase subunit I (COI) gene and COI barcoding

Zhao

Pan

et al. 2011

Animal

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DNA barcoding is an effective technique to identify species and analyze phylogenesis and evolution. However, research on and application of DNA barcoding in Canis have not been carried out. In this study, we analyzed two species of Canis, Canis lupus (n 5 115) and Canis latrans (n 5 4), using the cytochrome c oxidase subunit I (COI) gene (1545 bp) and COI barcoding (648 bp DNA sequence of the COI gene). The results showed that the COI gene, as the moderate variant sequence, applied to the analysis of the phylogenesis of Canis members, and COI barcoding applied to species identification of Canis members. Phylogenetic trees and networks showed that domestic dogs had four maternal origins (A to D) and that the Tibetan Mastiff originated from Clade A; this result supports the theory of an East Asian origin of domestic dogs. Clustering analysis and networking revealed the presence of a closer relative between the Tibetan Mastiff and the Old English sheepdog, Newfoundland, Rottweiler and Saint Bernard, which confirms that many well-known large breed dogs in the world, such as the Old English sheepdog, may have the same blood lineage as that of the Tibetan Mastiff.

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Section: Resultssupporting

confidence: 93%

Section: Resultsmentioning

confidence: 73%

Section: Resultsmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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The origin of the Tibetan Mastiff and species identification of Canis based on mitochondrial cytochrome c oxidase subunit I (COI) gene and COI barcoding

Zhao

Pan

et al. 2011

Animal

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“…The first Canine mitochondrial genome (from Sapsaree, a Korean native dog) was sequenced in 1998 (Kim et al, 1998), and subsequently the mitochondrial genomes of 25 domestic breed dogs, the gray wolf (Canis lupus; Bjö rnerfeldt et al, 2006), the coyote (Canis latrans; Bjö rnerfeldt et al, 2006) and the red fox (Vulpes vulpes; Arnason et al, 2006) have been sequenced. The partial mitochondrial genome of the Tibetan Mastiff has been reported (Li et al, 2008;Webb and Allard, 2009); however, the genome characteristics and differences from other domestic dogs were not made clear. Therefore, we sequenced the complete mitochondrial genome of the Tibetan Mastiff and analyzed its characteristics in comparison to those of other Canidae.…”

Section: Introductionmentioning

confidence: 99%

Complete sequence of the Tibetan Mastiff mitochondrial genome and its phylogenetic relationship with other Canids (Canis, Canidae)

Zhao

et al. 2011

Animal

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In this study, the complete sequence of the Tibetan Mastiff mitochondrial genome (mtDNA) was determined, and the phylogenetic relationships between the Tibetan Mastiff and other species of Canidae were analyzed using the coyote (Canis latrans) as an outgroup. The complete nucleotide sequence of the Tibetan Mastiff mtDNA was 16 710 bp, and included 22 tRNA genes, 2S rRNA gene, 13 protein-coding genes and one non-coding region (D-loop region), which is similar to other mammalian mitochondrial genomes. The characteristics of the protein-coding genes, non-coding region, tRNA and rRNA genes among Canidae were analyzed in detail. Neighbor-joining and maximum-parsimony trees of Canids constructed using 12 mitochondrial protein-coding genes showed that as the coyotes and Tibetan wolves clustered together, so too did the gray wolves and domestic dogs, suggesting that the Tibetan Mastiff originated from the gray wolf as did other domestic dogs. Domestic dogs clustered into four clades, implying at least four maternal origins (A to D). The Tibetan Mastiff, which belongs to clade A, appears to be closely related to the Saint Bernard and the Old English Sheepdog.

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Studies of modern Italian dog populations reveal multiple patterns for domestic breed evolution

Talenti

Dreger

Frattini

et al. 2018

Ecology and Evolution

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Through thousands of years of breeding and strong human selection, the dog (Canis lupus familiaris) exists today within hundreds of closed populations throughout the world, each with defined phenotypes. A singular geographic region with broad diversity in dog breeds presents an interesting opportunity to observe potential mechanisms of breed formation. Italy claims 14 internationally recognized dog breeds, with numerous additional local varieties. To determine the relationship among Italian dog populations, we integrated genetic data from 263 dogs representing 23 closed dog populations from Italy, seven Apennine gray wolves, and an established dataset of 161 globally recognized dog breeds, applying multiple genetic methods to characterize the modes by which breeds are formed within a single geographic region. Our consideration of each of five genetic analyses reveals a series of development events that mirror historical modes of breed formation, but with variations unique to the codevelopment of early dog and human populations. Using 142,840 genome‐wide SNPs and a dataset of 1,609 canines, representing 182 breeds and 16 wild canids, we identified breed development routes for the Italian breeds that included divergence from common populations for a specific purpose, admixture of regional stock with that from other regions, and isolated selection of local stock with specific attributes.

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Origin and phylogenetic analysis of Tibetan Mastiff based on the mitochondrial DNA sequence

Cited by 21 publications

References 18 publications

The origin of the Tibetan Mastiff and species identification of Canis based on mitochondrial cytochrome c oxidase subunit I (COI) gene and COI barcoding

The origin of the Tibetan Mastiff and species identification of Canis based on mitochondrial cytochrome c oxidase subunit I (COI) gene and COI barcoding

Complete sequence of the Tibetan Mastiff mitochondrial genome and its phylogenetic relationship with other Canids (Canis, Canidae)

Studies of modern Italian dog populations reveal multiple patterns for domestic breed evolution

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