Origin and Prevalence of Campylobaeter jejuni in Poultry Processing

SUMMARYThe genus Campylobacter is of great importance to public health because it includes several species that may cause diarrhea. These species may be found in water, food and in the intestinal tract of chickens. This study investigated the presence of Campylobacter jejuni and Campylobacter coli in chicken abattoirs in São Paulo State, Brazil. A total of 288 samples of feces, feathers, scald water, evisceration water, chiller water, and the rinse water of eviscerated, not eviscerated and chilled carcasses were collected in six chicken abattoirs. Polymerase Chain Reaction (PCR) was performed in Campylobacter spp.-positive isolates using the gene HIP, specific for hippuricase enzyme from Campylobacter jejuni and aspartokinase gene, specific to detect Campylobacter coli. The percentage of positive isolates of Campylobacter jejuni was 4.9% (14/288). Isolation was greater in feces samples (22%, 8/36). One sample was positive for the species C. coli. In conclusion, the results indicate that it is necessary to improve quality control for Campylobacter spp. in chicken abattoirs.

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“…The scalding at 58 °C decreased Campylobacter spp. contamination in the carcasses, but did not eliminate it completely 18 .…”

Section: Discussionmentioning

confidence: 99%

Survey of chicken abattoir for the presence of Campylobacter jejuni and Campylobacter coli

Cortez

Carvalho

Scarcelli

et al. 2006

Rev. Inst. Med. trop. S. Paulo

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“…Campylobacteriosis is mainly a food-borne infection, and poultry products may play an important role in the transmission to humans [1,8,10,21]. C. jejuni contamination of poultry meat during processing has been well documented [2,11,18,24]. During the slaughter, poultry carcasses become contaminated by the release of intestinal contents or transfer of contamination from the surface flora of the birds [11].…”

Section: Methodsmentioning

confidence: 99%

Analysis of Distribution of Campylobacter jejuni and Campylobacter coli in Broilers by Using Restriction Fragment Length Polymorphism of Flagellin Gene.

Chuma

Makino

Okamoto

et al. 1997

The Journal of Veterinary Medical Science

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ABSTRACT. The incidence of Campylobacter jejuni and Campylobacter coli in broiler farms was 33.9% (19/56). C. jejuni-positive flocks accounted for 20.0% (17/85) and C. coli-positive ones was 4.7% (4/85). There were 14 patterns (fla type) of restriction fragment length polymorphism (RFLP) of flagellin A gene among these 22 strains of C. jejuni and C. coli including the standard strain C. jejuni ATCC 33560. Different fla types of Campylobacter were isolated from broilers in different growing cycles on the same farms. Four strains of C. jejuni were isolated from four breeder farms and four fla types of C. jejuni were detected from their progenies reared on growing farms. Three fla types of C. jejuni detected from the progenies were different from those of each breeder. Also, the other three fla types of C. jejuni were detected from different progenies of each growing farm during the next growing cycle. These findings indicate that the RFLP analysis may contribute to epidemiological studies of C. jejuni and C. coli contamination of broilers and suggest the risk of contamination with different types of Campylobacter in every growing cycle of broilers on the farm even on the same farm. They also supported that there was little likeliness of the vertical transmission of C. jejuni and C. coli from breeders to broilers. -KEY WORDS : broiler, Campylobacter jejuni, flagellin gene typing, PCR, restriction fragment length polymorphism.

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“…The birds are healthy carriers of this bacterium, which may be found at counts of 106-109 colony forming units per gram of faeces [1,2]. Campylobacter colonization of live poultry may affect public health in two ways: (1) direct effect of the organism causing disease in workers at farms or processing plants [3,4], and (2) contamination of consumer-ready poultry products, which in turn may cause food-borne illness [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Epidemiology ofCampylobacterspp. at two Dutch broiler farms

Jacobs-Reitsma¹,

Giessen

Bolder³

et al. 1995

Epidemiol. Infect.

230

191

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SUMMARYBroiler flocks on two Dutch poultry farms were screened weekly for the presence of campylobacter in fresh caecal droppings during eight consecutive production cycles. Hatchery and fresh litter samples were taken at the start of each new cycle. Water, feed, insects, and faeces of domestic animals, present on the farms were also included in the sampling. Penner serotyping of isolates was used to identify epidemiological factors that contribute to campylobacter colonization in the broiler flocks. Generally, broiler flocks became colonized with campylobacter at about 3-4 weeks of age with isolation percentages of 100 %, and stayed colonized up to slaughter. A similar pattern of serotypes was found within the various broiler houses on one farm during one production cycle. New flocks generally showed also a new pattern of serotypes. Most serotypes isolated from the laying hens, pigs, sheep and cattle were different from those isolated from the broilers at the same time. Campylobacter serotypes from darkling beetles inside the broiler houses were identical to the ones isolated from the broilers. No campylobacter was isolated from any of the hatchery, water, feed or fresh litter samples. Conclusive evidence of transmission routes was not found, but results certainly point towards horizontal transmission from the environment. Horizontal transmission from one broiler flock to the next one via a persistent contamination within the broiler house, as well as vertical transmission from breeder flocks via the hatchery to progeny, did not seem to be very likely.

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Origin and Prevalence of Campylobaeter jejuni in Poultry Processing

Cited by 177 publications

References 0 publications

Survey of chicken abattoir for the presence of Campylobacter jejuni and Campylobacter coli

Survey of chicken abattoir for the presence of Campylobacter jejuni and Campylobacter coli

Analysis of Distribution of Campylobacter jejuni and Campylobacter coli in Broilers by Using Restriction Fragment Length Polymorphism of Flagellin Gene.

Epidemiology ofCampylobacterspp. at two Dutch broiler farms

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