Origin and Regenerative Potential of Vertebrate Mechanoreceptor-Associated Stem Cells

Widera, Darius; Hauser, Stefan; Kaltschmidt, Christian; Kaltschmidt, Barbara

doi:10.1155/2012/837626

Cited by 4 publications

(8 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In general, most of the oral and certain facial structures derive from the NC (Widera et al, 2012). However, whether the cells originating from the NC are retained in older adult animals is not known.…”

Section: Gingival Fibroblasts Derive From Neural Crestmentioning

confidence: 99%

Characterisation of human gingival neural crest-derived stem cells in monolayer and neurosphere cultures

Fournier

Loison-Robert²,

Ferre³

et al. 2016

eCM

View full text Add to dashboard Cite

Neural crest (NC)-derived stem cells (NCSC) have an exceptionally wide differentiation potential, but their use in regenerative therapy has been hampered by their scarcity in adult tissues and complex isolation protocols. Human oral mucosal gingiva may provide an attractive source of these cells as it contains NC-derived cells, the tissue is easily accessible and wound healing is fast and scarless with very little morbidity. To this end, we first investigated whether NC-derived cells are retained in adult gingiva by examining 8-months-old NC-reporter Wnt1-Cre/R26RYFP mice. We then hypothesised that gingival cell NC-like phenotype can be further enhanced by floating neurosphere cultures generated from standard human gingival fibroblast (GF) and pooled CFU-F (GSC) cultures. Findings showed that NC-derived cells are retained in the gingival connective tissue of aged mice. Human GFs and GSCs expressed NC-related genes nestin, Snai1, Twist1, Pax3, Sox9 and FoxD3, and generated neurospheres. This was mediated via calcium-and connexin 43-dependent cell communication, which is similar to neurospheres formed by neural progenitors. Cells in the spheres showed significantly increased expression of NC-related genes, and down regulation of fibroblast-related type I collagen. Structurally, the neurospheres were polarised with nestin positive cells located on the outer layers underlined with an extracellular matrix rich in molecules typical to embryonic NC. Sphere-derived cells expressed significantly elevated levels of neural markers, and differentiated into Tau, neurofilament-M and GFAP-positive cells suggesting neural differentiation potential. Thus, human GF and GSC cultures may provide an efficient source of NC-derived cells via enrichment by floating sphere cultures.

show abstract

Section: Gingival Fibroblasts Derive From Neural Crestmentioning

confidence: 99%

Characterisation of human gingival neural crest-derived stem cells in monolayer and neurosphere cultures

Fournier

Loison-Robert²,

Ferre³

et al. 2016

eCM

View full text Add to dashboard Cite

show abstract

“…Both types are di erently distributed in the skin (and body), with di erent morphologies, but both consist of connective-tissue-encapsulated Schwann cell lamellae. Merkel cell-neurite complexes and Meissner's corpuscles have been shown to regenerate a er skin damage (reviewed in: Widera et al, 2012)). Explanations of the regenerative capacity range from the presence of neural crest stem cells distributed in the skin Nagase et al, 2007) to the dynamic plasticity of Schwann cells .…”

Section: Sensorsmentioning

confidence: 99%

“…Some of the TRP channels may be multimodal, also responding to mechanical and chemical stimuli ). (Widera et al, 2012). As always, more studies are needed before we can fully understand the complexities of biology.…”

Section: Sensorsmentioning

confidence: 99%

See 1 more Smart Citation

Fibroblast Differentiation and Models of Human Skin

Rakar¹

2016

Linköping University Medical Dissertations

View full text Add to dashboard Cite

Denna bok avhandlar en riktning av biomedicinsk forskning som i det långa loppet sy ar till att nå bättre och banbrytande sätt att läka våra kroppar. Forskningen vilar på en tredelad grund -viktiga kunskaper som leder mot en generell förståelse för hur celler blir till vävnader, hur vävnad-er och organ kan manipuleras på ett kliniskt användbart sätt, och till sist hur denna kunskap kan appliceras på människans hudorgan.Den första delen handlar om cellers identitet, och den stora potentialen som våra egna stamceller har för framtidens läkekonst. Genom att förstå hur celler egentligen fungerar på arvsmassenivå kan vi utöka arsenalen av verktyg vi har för att få celler att bete sig på de sätt som behövs för att skapa nya organ. Vi har jobbat med att karakterisera och manipulera broblaster, en av de vanligaste celltyperna i människokroppen, och bland annat tagit fram olika typer av fettceller.Den andra delen handlar om hur man använder celler, biokemiska faktorer och biokompatibla material för att konstruera vävnader och organ. Innan forskningen når klinisk användbarhet har vi möjlighet att bygga begränsade modellsystem för att studera människans biologi. Cellsystem på labbet är mycket viktiga verktyg för att föra forskningen på vävnadsbyg-gande framåt.Den tredje delen behandlar hudforskning speci kt, och här samlas kunskaper från tidigare delarna som är relevanta för mänsklig hud. Här beskrivs användningen av två olika hudmodeller för att undersöka proceser viktiga vid sårläkning. Vidare beskrivs planerna kring ett större påbörjat projekt som sy ar till att använda alla tidigare lärdomar och tekniker för att åstadkomma en ny terapi för sårläkning.Abstract is thesis combines three publications and one manuscript, covering two principal topics: functional di erentiation of human broblasts and, laboratory models of human skin. e two topics favourably unite in the realm of tissue engineering. is thesis is therefore split into three main parts: 1. a discussion of phenotypic plasticity as it pertains to broblasts and the stem cell continuum; 2. a short review of engineered tissue, with particular focus on soluble factors and materials; and, 3. a motivated review of the biology, diversity and culture of skin, including skin construction. e intended goal of our research endeavor was to achieve the formulation of a bioactive therapy for skin regeneration. e main hypothesis was that broblast-to-keratinocyte di erentiation would facilitate wound healing, and that the protocol for such a method could be adapted to clinical translation. e foundation for the hypothesis lay in the di erentiation capabilities of primary dermal broblasts (Paper I). However, the goal has not yet been achieved. Instead, intermediate work on the construction of skin for the purpose of creating a model test-bed has resulted in two other publications. e use of excised human skin, a formidable reference sample for tissue engineered skin, has been used to investigate a gelatin-based material in re-epithelialization (Paper II). A rst attempt at standardizi...

show abstract

“…The origin of regenerative cells remains an active area of research in the developmental biology field (Bourin et al, ; Vincent and Buckingham, ; Widera et al, ). While humans have a limited capacity to regenerate tissues, primarily liver and skeletal muscle (Schaffner, ; Jarvinen et al, ), other vertebrate species have extensive regenerative capacity and have provided great insight into the molecular mechanisms involved (Stoick‐Cooper et al, ; Brockes and Kumar, ) and a number of common principles in cellular recruitment and differentiation during regeneration have been identified.…”

Section: Current Regenerative Medicine Strategiesmentioning

confidence: 99%

Lessons from developmental biology for regenerative medicine

Turner

Keane

Badylak

2013

Birth Defects Research Pt C

View full text Add to dashboard Cite

The ultimate goal of regenerative medicine is the functional restoration of lost or damaged tissues and organs. Since most tissues in man lack true regenerative properties and instead respond to injury with an inflammatory response and scar tissue formation, regenerative medicine strategies that include combinations of cells, scaffolds, and bioactive molecules to replace injured or missing tissues have been developed. The physical, chemical, and electrical cues that define the microenvironmental niche and the effect of these influences upon cell behavior during development are of interest to developmental biologists, with obvious overlap to the interest of the regenerative medicine field. This manuscript provides an overview of current approaches for tissue restoration being investigated in the field of regenerative medicine and attempts to identify areas of mutual beneficial interest with the field of developmental biology.

show abstract

Origin and Regenerative Potential of Vertebrate Mechanoreceptor-Associated Stem Cells

Cited by 4 publications

References 70 publications

Characterisation of human gingival neural crest-derived stem cells in monolayer and neurosphere cultures

Characterisation of human gingival neural crest-derived stem cells in monolayer and neurosphere cultures

Fibroblast Differentiation and Models of Human Skin

Lessons from developmental biology for regenerative medicine

Contact Info

Product

Resources

About