Origin and renewal of goblet cells in the epithelium of the mouse small intestine

Merzel, J; Leblond, C. P.

doi:10.1002/aja.1001240303

Cited by 115 publications

(47 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The nucleus which is flattened along the base of the goblet appeares quite normal. Globules with varying amounts of surrounding membrane are still attached to may occur during the "migration" from the depth of the crypts to the tips of the villi (BIZZORERO, 1892;MESSIER and LEBLOND, 1960;MERZEL and LEBLOND, 1969). The goblet cells in this study were from the villi and therefore were approaching the end of their migration.…”

Section: Discussionmentioning

confidence: 88%

The Ultrastructure of the Goblet Cell in the Dog Ileum at the Later Stage of Secretion

Makita

Kiwaki

Sandborn

et al. 1972

Arch. Histol. Jap., Arch. Histol. Jpn

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 88%

The Ultrastructure of the Goblet Cell in the Dog Ileum at the Later Stage of Secretion

Makita

Kiwaki

Sandborn

et al. 1972

Arch. Histol. Jap., Arch. Histol. Jpn

View full text Add to dashboard Cite

“…Gastric epithelial turnover displayed an arithmetic WX of 3.05 (n = 19) days, lacking reported variance (Fig. 3) (Leblond and Messier, 1958;Walker and Leblond, 1958;Quastler and Sherman, 1959;Creamer et al, 1961;Fry et al, 1961Fry et al, , 1962Lesher et al, 1961;Lipkin and Quastler, 1962;Thrasher, 1967;Grey, 1968;Merzel and Leblond, 1969;Aluwihare, 1971;Leblond, 1971, 1974;Bottomley and Cooper, 1973;Kovacs and Potten, 1973;Potten et al, 1974;Chang and Nadler, 1975; Richards, 1977;de Rodriguez et al, 1979;Tsubouchi, 1981;Baril et al, 1982;Cheng and Bjerknes, 1983;Smith et al, 1984;Lee, 1985;Thompson et al, 1990;Ferraris et al, 1992;Leblond, 1993a, 1993b;Nowacki, 1993).…”

Section: Enterocyte Turnover In Preclinical Speciesmentioning

confidence: 95%

Meta-Analysis of the Turnover of Intestinal Epithelia in Preclinical Animal Species and Humans

et al. 2014

View full text Add to dashboard Cite

Due to the rapid turnover of the small intestinal epithelia, the rate at which enterocyte renewal occurs plays an important role in determining the level of drug-metabolizing enzymes in the gut wall. Current physiologically based pharmacokinetic (PBPK) models consider enzyme and enterocyte recovery as a lumped first-order rate. An assessment of enterocyte turnover would enable enzyme and enterocyte renewal to be modeled more mechanistically. A literature review together with statistical analysis was employed to establish enterocyte turnover in human and preclinical species. A total of 85 studies was identified reporting enterocyte turnover in 1602 subjects in six species. In mice, the geometric weighted combined mean (WX) enterocyte turnover was 2.81 6 1.14 days (n = 169). In rats, the weighted arithmetic mean enterocyte turnover was determined to be 2.37 days (n = 501). Humans exhibited a geometric WX enterocyte turnover of 3.48 6 1.55 days for the gastrointestinal epithelia (n = 265), displaying comparable turnover to that of cytochrome P450 enzymes in vitro (0.96-4.33 days). Statistical analysis indicated humans to display longer enterocyte turnover as compared with preclinical species. Extracted data were too sparse to support regional differences in small intestinal enterocyte turnover in humans despite being indicated in mice. The utilization of enterocyte turnover data, together with in vitro enzyme turnover in PBPK modeling, may improve the predictions of metabolic drug-drug interactions dependent on enzyme turnover (e.g., mechanism-based inhibition and enzyme induction) as well as absorption of nanoparticle delivery systems and intestinal metabolism in special populations exhibiting altered enterocyte turnover.

show abstract

“…The granules of these rare cells also contain small amounts of mucin. Because of their morphologic features, they have been termed "intermediate" (13), "granulomucous" (46), or "transitional" (47,48) cells. These cells have been proposed to be Paneth cells undergoing transformation to goblet cells, goblet cells in the process of being converted to Paneth cells, or a precursor of both lineages.…”

Section: General Comments About Paneth Goblet and Intermediatementioning

confidence: 99%

Examining the Role of Paneth Cells in the Small Intestine by Lineage Ablation in Transgenic Mice

Garabedian

Roberts

McNevin

et al. 1997

Journal of Biological Chemistry

241

188

View full text Add to dashboard Cite

The structural and functional organization of the adult mouse small intestinal epithelium lends itself to studying both the regulation and integration of cellular proliferation, differentiation, and death programs. The epithelium contains four principal cell types: absorptive enterocytes (comprising Ͼ80% of the total population), enteroendocrine cells, mucus-producing goblet cells, and Paneth cells. All four lineages are derived from a multipotent stem cell that is functionally anchored near the base of each of the small intestine's 1.1 million crypts of Lieberkü hn (1-4). Cell division is confined to these crypts (5). Enterocytes, enteroendocrine, and goblet cells migrate out of the crypt and up an adjacent villus. Migration is highly ordered and associated with terminal differentiation. Cell death occurs near the villus tip where cells are exfoliated into the lumen (6, 7). Proliferation, differentiation, and death take place in a spatially well-organized continuum that extends from the crypt to the apex of a villus. This sequence is completed rapidly (2-5 days in the case of enterocytes, enteroendocrine, and goblet cells; Refs. 1 and 8 -10) and is recapitulated throughout the lifespan of the mouse.The Paneth cell lineage differs from the others in a number of notable ways. It is the only lineage that executes its terminal differentiation program during a downward migration from the stem cell zone to the crypt base (11). It is the longest lived lineage, and the only one that exists entirely within the proliferative compartment. Each crypt contains 30 -50 mature Paneth cells that survive for 18 -23 days before degenerating and undergoing phagocytosis by their neighbors (11-13). Paneth cell age correlates with position in the crypt; the most mature cells are located at or near the crypt base (2). The size of the Paneth cell's apical secretory granules also correlates with age; larger granules are produced by older cells (2, 11).The function of the Paneth cell has not yet been clearly defined. Residency at the crypt base places this lineage in a position to release products from its apical granules that could affect establishment and/or maintenance of the stem cell's niche or influence the properties of the stem cell's descendants. A number of factors exported by Paneth cells could regulate epithelial proliferation and differentiation programs. They include tumor necrosis factor-␣ (14), guanylin (15), and epidermal growth factor (16). Two Paneth cell products have been implicated as modifiers of adenoma formation in mice heterozygous for a mutation in the adenomatous polyposis coli gene, Apc Min (17). Production of matrilysin, a matrix metalloprotein-

show abstract

Origin and renewal of goblet cells in the epithelium of the mouse small intestine

Cited by 115 publications

References 20 publications

The Ultrastructure of the Goblet Cell in the Dog Ileum at the Later Stage of Secretion

The Ultrastructure of the Goblet Cell in the Dog Ileum at the Later Stage of Secretion

Meta-Analysis of the Turnover of Intestinal Epithelia in Preclinical Animal Species and Humans

Examining the Role of Paneth Cells in the Small Intestine by Lineage Ablation in Transgenic Mice

Contact Info

Product

Resources

About