Origin and turnover of ECM proteins from the inner limiting membrane and vitreous body

Halfter, Willi; Dong, Sucai; Dong, Aling; Eller, Andrew W.; Nischt, Roswitha

doi:10.1038/eye.2008.19

Cited by 90 publications

(63 citation statements)

References 36 publications

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“…Previous AFM studies showed that the mechanical strength is in the mPa range, very similar to the articular cartilage and about 1000-fold stronger than cell layers. 18,24,25 Our results underline previous reports 20 describing that the average stiffness of the ILM is over fivefold higher at the retinal side as compared with the vitreal side. This difference nicely explains the fact that the ILM curls up towards its vitreal side after peeling.…”

Section: Discussionsupporting

confidence: 81%

“…15 Atomic Force Microscopy (AFM) is a well established examination technique that has been used to measure the thickness and rigidity of human ocular basement membranes such as the unstained ILM. 18,19 The aim of the present investigation was to assess and quantify in vitro changes of the ILM of the human retina after staining with either brilliant blue or ICG with and without subsequent illumination using a standard surgical light (PENTA LUX x 50, Ophthalmologische Systeme GmbH Fritz Ruck, Eschweiler, Germany) source compared with unstained ILM using AFM.…”

Section: Introductionmentioning

confidence: 99%

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Vital dyes increase the rigidity of the internal limiting membrane

Haritoglou

Mauell

Benoit

et al. 2013

Eye

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Purpose To assess the stiffness of the natural human internal limiting membrane (ILM) and evaluate potential changes of the mechanical properties following staining with brilliant blue (BB) and indocyanine green (ICG). Methods Unstained ILM specimens were obtained during ophthalmic surgical procedures. After removal, the specimens were dissected into five parts. Two fragments were stained with BB and ICG, respectively, for 1 min, another two specimens were stained similarly followed by additional subsequent illumination using a standard light source (PENTA LUX x 50, Ophthalmologische Systeme GmbH Fritz Ruck). The fifth part served as an untreated control. All specimens were then analyzed using atomic force microscopy (AFM) in contact mode with a scan rate of 0.6 Hz. Two scan regions of 10 Â 10 mm were chosen and stiffness was determined by using AFM in a force spectroscopy mode. The force curves were plotted with a data rate of 5000 Hz. In all specimens both the retinal side and vitreal side were analyzed.

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Section: Discussionsupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Vital dyes increase the rigidity of the internal limiting membrane

Haritoglou

Mauell

Benoit

et al. 2013

Eye

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“…These differences may in part reflect the way each basement membrane forms, for example, the radial glial endfeet and the meningeal fibroblasts are thought to deposit the pial basement membrane on the surface of the brain, whereas the ILM forms by the precipitation of matrix proteins from the vitreous; a process thought to allow for the rapid assembly of the ILM during embryonic eye development (Halfter et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Fukutin-Related Protein Alters the Deposition of Laminin in the Eye and Brain

Ackroyd¹,

Whitmore²,

Prior³

et al. 2011

J. Neurosci.

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Mutations in fukutin-related protein (FKRP) are responsible for a common group of muscular dystrophies ranging from adult onset limb girdle muscular dystrophies to severe congenital forms with associated structural brain involvement. The defining feature of this group of disorders is the hypoglycosylation of ␣-dystroglycan and its inability to effectively bind extracellular matrix ligands such as laminin ␣2. However, ␣-dystroglycan has the potential to interact with a number of laminin isoforms many of which are basement membrane/ tissue specific and developmentally regulated. To further investigate this we evaluated laminin ␣-chain expression in the cerebral cortex and eye of our FKRP knock-down mouse (FKRP KD ). These mice showed a marked disturbance in the deposition of laminin ␣-chains including ␣1, ␣2, ␣4, and ␣5, although only laminin ␣1-and ␥1-chain mRNA expression was significantly upregulated relative to controls. Moreover, there was a diffuse pattern of laminin deposition below the pial surface which correlated with an abrupt termination of many of the radial glial cells. This along with the pial basement membrane defects, contributed to the abnormal positioning of both early-and late-born neurons. Defects in the inner limiting membrane of the eye were associated with a reduction of laminin ␣1 demonstrating the involvement of the ␣-dystroglycan:laminin ␣1 axis in the disease process. These observations demonstrate for the first time that a reduction in Fkrp influences the ability of tissue-specific forms of ␣-dystroglycan to direct the deposition of several laminin isoforms in the formation of different basement membranes.

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“…A primary role for the CB is secretion of the aqueous humor, and of components of the vitreous and inner limiting membranes. The CB is therefore pivotal to the establishment and maintenance of ocular pressure and to the survival of the GCs (Gould et al, 2004;Halfter et al, 2008;Halfter et al, 2005). The iris is composed of two layers of pigmented epithelium, muscles and stroma (for a review, see Davis-Silberman and Ashery-Padan, 2008).…”

Section: Introductionmentioning

confidence: 99%

Roles for Dicer1 in the patterning and differentiation of the optic cup neuroepithelium

2011

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SUMMARYThe embryonic ocular neuroepithilium generates a myriad of cell types, including the neuroretina, the pigmented epithelium, the ciliary and iris epithelia, and the iris smooth muscles. As in other regions of the developing nervous system, the generation of these various cell types requires a coordinated sequence of patterning, specification and differentiation events. We investigated the roles of microRNAs (miRNAs) in the development of optic cup (OC)-derived structures. We inactivated Dicer1, a key mediator of miRNA biosynthesis, within the OC in overlapping yet distinct spatiotemporal patterns. Ablation of Dicer1 in the inner layer of the OC resulted in patterning alteration, particularly at the most distal margins. Following loss of Dicer1, this region generated a cryptic population of cells with a mixed phenotype of neuronal and ciliary body (CB) progenitors. Notably, inactivation of Dicer1 in the retinal progenitors further resulted in abrogated neurogenesis, with prolongation of ganglion cell birth and arrested differentiation of other neuronal subtypes, including amacrine and photoreceptor cells. These alterations were accompanied by changes in the expression of Notch and Hedgehog signaling components, indicating the sensitivity of the pathways to miRNA activity. Moreover, this study revealed the requirement of miRNAs for morphogenesis of the iris and for the regulation of CB cell type proliferation and differentiation. Together, analysis of the three genetic models revealed novel, stage-dependent roles for miRNAs in the development of the ocular sub-organs, which are all essential for normal vision.

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Origin and turnover of ECM proteins from the inner limiting membrane and vitreous body

Cited by 90 publications

References 36 publications

Vital dyes increase the rigidity of the internal limiting membrane

Vital dyes increase the rigidity of the internal limiting membrane

Fukutin-Related Protein Alters the Deposition of Laminin in the Eye and Brain

Roles for Dicer1 in the patterning and differentiation of the optic cup neuroepithelium

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