Origin, Development, and Nature of Intranuclear Rodlets and Associated Bodies in Chicken Sympathetic Neurons

Abstract

Correlative data are presented here on the developmental history, dynamics, histochemistry, and fine structure of intranuclear rodlets in chicken sympathetic neurons from in vivo material and long-term organized tissue cultures . The rodlets consist of bundles of -70 t 10 A proteinaceous filaments closely associated with -0 .4-0 .8 µ spheroidal, granulofibrillar (gf) bodies of a related nature . These bodies are already present in the developing embryo a week or more in advance of the rodlets . In early formative stages rodlets consist of small clusters of aligned filaments contiguous with the gf-bodies. As neuronal differentiation progresses these filaments increase in number and become organized into well-ordered polyhedral arrays . Time-lapse cinemicrography reveals transient changes in rodlet contour associated with intrinsic factors, changes in form and position of the nucleolus with respect to the rodlet, and activity of the gf-bodies . With the electron microscope filaments may be seen extending between the nucleolus, gf-bodies, and rodlets ; nucleoli display circumscribed regions with fine structural features and staining reactions reminiscent of those of gf-bodies . We suggest that the latter may be derivatives of the nucleolus and that the two may act together in the assemblage and functional dynamics of the rodlet . The egress of rodlet filaments into the cytoplasm raises the possibility that these might represent a source of the cell's filamentous constituents .

Protein-synthesis inhibition induces perichromatin granule accumulation and intranuclear rodlet formation in osmotically stimulated supraoptic neurons

“…Cytochemical studies have demonstrated the proteinaceous nature of intranuclear rodlets (Masurovsky et al 1970). Since different cytoskeletal proteins such as actin, tubulin and myosin have been identified as endogenous nuclear proteins (Douvas et al 1975;LeStourgeon et al 1975;Schindler and Jiang 1986), intranuclear rodlets might be formed by local assembly of diffusible forms of such cytoskeletal proteins.…”

Nuclear changes in colo-rectal epithelium with special reference to nuclear inclusions in carcinoma, dysplasia, adenoma and Peutz-Jeghers polyps.

“…Nevertheless, nuclear inclusions (NIs) other than nuclear bodies have not been sufficiently reported except for neural tissues (Masurovsky et al 1970;Vazquez et al 1970;Tani 1971;Shaw and Sumi 1975) and endocrine organs (Boquist 1969;Dahl 1970;Sherwin et al 1977; Ryder et al 1979; DeLellis et al 1980). Through a systematic observation of several polypoid lesions and carcinomas of the large bowel, we found a number of nuclear inclusions.…”

Intranuclear inclusions in the bone marrow cells of miniature pigs.

“…Figure 9 shows an electron micrograph of a neutrophil leukocyte treated with 0.5% pepsin for 30min the intranuclear inclusions remained undisturbed. HIRANO and ZIMMERMAN, 1967;POPOFF and STEWART, 1968;MASUROVSKY et al, 1970) noted the filamentous structure in similar nuclear inclusions in neurons. Cell Tiss.…”

“…According to these authors, there are three types of intranuclear inclusions which differ in ultrastructure from each other: filamentous (MASUROVSKY et al, 1970;WARCHOL, 1978;SEITE et al, 1979), microtubular (SEITE et al, 1979) and crystalline (CHANDLER and WILLIS, 1966;PATRIZI and MIDDELKAMP, 1969;LAFARGA and PALACIOS, 1979 (MAGALHAES, 1967), the dog epididymidis (GOURANTON et al, 1979), the rat pineal (BOQUIST, 1969 …”

“…The presence of intranuclear inclusions in nerve cells has been reported in human cerebral neurons (ROBERTSON and MACLEAN, 1965;RAINE and FIELD, 1968), rat brain neurons (CHANDLER and WILLIS, 1966;SOTELO and PALAY, 1968;WARCHOL, 1978;LAFARGA and PALACIOS, 1979), the rabbit olfactory bulb (SIEGESMUND et al, 1964), golden hamster central nervous tissue (POPOFF and STEWERT, 1968), cat sympathetic neurons (SEITE et al, 1979), dog sphenopalatine ganglion cells (COSTA and PAULA-BARSOSA, 1979), and chicken sympathetic neurons (KIM et al, 1970). According to these authors, there are three types of intranuclear inclusions which differ in ultrastructure from each other: filamentous (MASUROVSKY et al, 1970;WARCHOL, 1978;SEITE et al, 1979), microtubular (SEITE et al, 1979) and crystalline (CHANDLER and WILLIS, 1966;PATRIZI and MIDDELKAMP, 1969;LAFARGA and PALACIOS, 1979 (MAGALHAES, 1967), the dog epididymidis (GOURANTON et al, 1979), the rat pineal (BOQUIST, 1969 …”