Origin of Dissolved-phase Hyperpolarized 129Xe Signal in the Mouse Chest Based on Experimental Evidence from Extensive Magnetic Resonance Measurements

Narazaki, Michiko; Kimura, Atsuomi; Wakayama, Teruhiko; Imai, Hirohiko; Fujiwara, Hideaki

doi:10.2463/mrms.10.149

Cited by 9 publications

(4 citation statements)

References 25 publications

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“…Based on their signal dynamics, and the limited available literature, we can postulate the origins of these peaks. We suggest that the 198.4 ppm peak originates from blood (13,40), and the 195.5 and 193.9 ppm peaks from aqueous media (pulmonary-capillary barrier tissue, plasma, and possibly the myocardium). The remaining upfield peaks at <192 ppm, which only appear at long 129 Xe replenishment times, arise from the slowly perfused and distal epicardial fat (3).…”

Section: Discussionmentioning

confidence: 87%

Establishing an accurate gas phase reference frequency to quantify¹²⁹Xe chemical shifts in vivo

et al. 2016

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Purpose 129Xe interacts with biological media to exhibit chemical shifts exceeding 200 ppm that report on physiology and pathology. Extracting this functional information requires shifts to be measured precisely. Historically, shifts have been reported relative to the gas-phase resonance originating from pulmonary airspaces. However, this frequency is not fixed—it is affected by bulk magnetic susceptibility, as well as Xe-N2, Xe-Xe, and Xe-O2 interactions. Here, we address this by introducing a robust method to determine the 0 ppm 129Xe reference from in vivo data. Methods Respiratory-gated hyperpolarized 129Xe spectra from the gas- and dissolved-phases were acquired in 4 mice at 2T from multiple axial slices within the thoracic cavity. Complex spectra were then fitted in the time domain to identify peaks. Results Gas-phase 129Xe exhibited two distinct resonances corresponding to 129Xe in conducting airways (varying from −0.6±0.2 to +1.3±0.3 ppm) and alveoli (relatively stable, at −2.2±0.1 ppm). Dissolved-phase 129Xe exhibited five reproducible resonances in the thorax at 198.4±0.4, 195.5±0.4, 193.9±0.2, 191.3±0.2, and 190.7±0.3 ppm. Conclusion The alveolar 129Xe resonance exhibits a stable frequency across all mice. Therefore, it can provide a reliable in vivo reference frequency by which to characterize other spectroscopic shifts.

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Section: Discussionmentioning

confidence: 87%

Establishing an accurate gas phase reference frequency to quantify¹²⁹Xe chemical shifts in vivo

et al. 2016

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“…It was reported that 5 dissolved 129 Xe peaks appeared in rat head spectra . Although the spectral pattern of the dissolved 129 Xe spectra seems to be different between mice and others, (those were confirmed from the HP 129 Xe MR spectroscopy in the lung , we assigned the dissolved 129 Xe peak from the brain tissue in mice as described below. According to the literature, the main HP 129 Xe signal dissolved in the rat brain appears at around 196 ppm .…”

Section: Resultsmentioning

confidence: 86%

Development of a fast method for quantitative measurement of hyperpolarized ¹²⁹Xe dynamics in mouse brain

et al. 2011

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A fast method has been established for the precise measurement and quantification of the dynamics of hyperpolarized (HP) xenon-129 ((129)Xe) in the mouse brain. The key technique is based on repeatedly applying radio frequency (RF) pulses and measuring the decrease of HP (129)Xe magnetization after the brain Xe concentration has reached a steady state due to continuous HP (129)Xe ventilation. The signal decrease of the (129)Xe nuclear magnetic resonance (NMR) signal was well described by a simple theoretical model. The technique made it possible to rapidly evaluate the rate constant α, which is composed of cerebral blood flow (CBF), the partition coefficient of Xe between the tissue and blood (λ(i)), and the longitudinal relaxation time (T(1i)) of HP (129)Xe in the brain tissue, without any effect of depolarization by RF pulses and the dynamics in the lung. The technique enabled the precise determination of α as 0.103 ± 0.018 s(-1) (± SD, n = 5) on healthy mice. To investigate the potential of this method for detecting physiological changes in the brain of a kainic acid (KA) -induced mouse model of epilepsy, an attempt was made to follow the time course of α after KA injection. It was found that the α value changes characteristically with time, reflecting the change in the physiological state of the brain induced by KA injection. By measuring CBF using (1)H MRI and (129)Xe dynamics simultaneously and comparing these results, it was suggested that the reduction of T(1i), in addition to the increase of CBF due to KA-induced epilepsy, are possible causes of the change in (129)Xe dynamics. Thus, the present method would be useful to detect a pathophysiological state in the brain and provide a novel tool for future brain study.

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“…Therefore, the FWHM value is considered as the higher limit for the component signal, which results in a lower limit of T

_{2}^{*}

. This situation is specific to mice, for which the spectral pattern of the 129 Xe dissolved phase is quite different from that for other species (9, 28, 29). Pixel‐based T

_{2}^{*}

values were calculated with the images with TE = 0.2, 0.6, and 1.0 ms obtained in one control mouse using a two parameter least‐squares fitting routine.…”

Section: Methodsmentioning

confidence: 99%

Erratum

2009

Cancer

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Both chemical and topographic cues are crucial for the development of skeletal muscle. In this study, the relative roles of both signals in regard to cell adhesion, morphology, and differentiation of C2C12 skeletal myoblasts were investigated. Grooved polystyrene substrates containing grooves with approximately 900 nm in width with 600 nm ridge spans and 665 nm in depth were conjugated with the cell adhesion peptide arginine–glycine–aspartic acid (RGD). RGD conjugation significantly enhanced the adhesion, growth and differentiation of C2C12 cells. On the other hand, anisotropic topography primarily directed the direction and alignment of myoblasts and myotubes. The results in this study provide information regarding the relative roles of chemical and topographic cues in musculoskeletal myogenesis, and are of interest to applications in muscle tissue engineering. Biotechnol. Bioeng. 2012; 109:2104–2115. © 2012 Wiley Periodicals, Inc.

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Origin of Dissolved-phase Hyperpolarized 129Xe Signal in the Mouse Chest Based on Experimental Evidence from Extensive Magnetic Resonance Measurements

Cited by 9 publications

References 25 publications

Establishing an accurate gas phase reference frequency to quantify¹²⁹Xe chemical shifts in vivo

Establishing an accurate gas phase reference frequency to quantify¹²⁹Xe chemical shifts in vivo

Development of a fast method for quantitative measurement of hyperpolarized ¹²⁹Xe dynamics in mouse brain

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Origin of Dissolved-phase Hyperpolarized 129Xe Signal in the Mouse Chest Based on Experimental Evidence from Extensive Magnetic Resonance Measurements

Cited by 9 publications

References 25 publications

Establishing an accurate gas phase reference frequency to quantify129Xe chemical shifts in vivo

Establishing an accurate gas phase reference frequency to quantify129Xe chemical shifts in vivo

Development of a fast method for quantitative measurement of hyperpolarized 129Xe dynamics in mouse brain

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Establishing an accurate gas phase reference frequency to quantify¹²⁹Xe chemical shifts in vivo

Establishing an accurate gas phase reference frequency to quantify¹²⁹Xe chemical shifts in vivo

Development of a fast method for quantitative measurement of hyperpolarized ¹²⁹Xe dynamics in mouse brain