Origin of Esterases in Human Whole Saliva

Lindqvist, L; Nord, Carl‐Erik; Söder, Per‐Östen

doi:10.1159/000458785

Cited by 26 publications

(11 citation statements)

References 17 publications

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“…Prchminary investigations have shown that the hydrolase activity in saliva bacteria is of minor importance for the overall activity and it is thus proposed that the mean esterase activity in sahva derives from epithehal cells. This is in accordance with observations by others (19).…”

Section: Discussionsupporting

confidence: 94%

Effect of human saliva on surface degradation of composite resins

Larsen

Munksgaard

1991

European J Oral Sciences

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– The amount of methacrylic acid produced by incubation of the dimethacrylate ester TEGDMA in human whole saliva was measured. The conversion rate of TEGDMA was determined in stimulated saliva from dental students and in unstimulated saliva from both dental students and patients. The differences in mean conversion rate measured in the three groups were not statistically significant (P= 0.346). The mean conversion rate ± SD for all saliva samples was 4.54 ± 3.24 μmol MAA/L. h. The surface of BISGMA/TEGDMA‐polymer specimens treated with porcine liver esterase in activities equivalent to those found in human saliva exhibited a lower Wallace microhardness than the surface of untreated specimens. Polymer specimens incubated in human saliva exhibited a similar reduction in the microhardness. The results demonstrated that enzymes in human saliva are capable of softening the surface of dimethaerylate polymers presumably by inducing a hydrolysis of methacrylate ester bonds. The mechanical removal of a surface layer softened by hydrolases will expose a new surface layer to enzymatic attack. The enzymatic softening may thus contribute to the in vivo wear of composite restorations.

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Section: Discussionsupporting

confidence: 94%

Effect of human saliva on surface degradation of composite resins

Larsen

Munksgaard

1991

European J Oral Sciences

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“…Human Salivary Derived Esterases (HSDE) are part of the salivary group of enzymes which show strong degradative activity toward the methacrylate-based organic matrix of dental composite resin. [1][2][3][4][5] HSDE catalyze the breakdown of one of the most common monomers used in dental composites and adhesives, 2,2-[4(2-hydroxy 3-methacryloxypropoxy)-phenyl] propane (Bis-GMA), to form the degradation product 2,2-bis [4 (2,3-hydroxy-propoxy)phenyl] propane (Bis-HPPP). 5,6 Bis-HPPP by-product was previously shown to be a true marker of resin matrix biodegradation.…”

Section: Introductionmentioning

confidence: 99%

Effect of salivary esterase on the integrity and fracture toughness of the dentin‐resin interface

et al. 2010

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Human Salivary Derived Esterases (HSDE) are part of the salivary group of enzymes which show strong degradative activity toward the breakdown of one of the most common monomers used in dental composites and adhesives, 2,2-[4(2-hydroxy 3-methacryloxypropoxy)-phenyl] propane (Bis-GMA), to form the degradation product 2,2-bis [4 (2,3-hydroxy-propoxy)phenyl] propane (Bis-HPPP). This study was aimed to evaluate the effects of HSDE on the biodegradation and fracture toughness of the adhesive resin-dentin interface. Adhesive resin (Scotchbond Multi Purposes), resin composite (Z250) and mini short-rod specimens, were either not incubated; or incubated in phosphate-buffered saline (PBS) or HSDE media for up to 180 days (37 degrees C, pH 7.0). The amount of Bis-HPPP was analyzed by high performance liquid chromatography and mini-SR specimens were tested for fracture toughness using universal testing machine following 30, 90, or 180-day incubation periods. Significantly higher amounts of Bis-HPPP were produced in HSDE than in PBS incubated specimens (p < 0.05). Non-incubated mini-SR specimens had the higher fracture-toughness values, while specimens incubated for 180-days in HSDE had the lowest fracture toughness (p < 0.05). This study suggests that biodegradation is an on-going clinically relevant process that progressively compromises the integrity of the critical resin restoration-adhesive interface, as well as the resin-composite component with time.

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“…One class of salivary components that has some interest in terms of composite biodegradation is the esterases (Lindqvist et al, 1977).…”

Section: Introductionmentioning

confidence: 99%

Salivary Esterase Activity and Its Association with the Biodegradation of Dental Composites

2004

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Pseudocholinesterase (PCE) and cholesterol esterase (CE) can hydrolyze bisphenylglycidyl dimethacrylate (bisGMA) and triethylene glycol dimethacrylate (TEGDMA) monomers. This study will test the hypothesis that enzyme activities showing CE and PCE character are found in human saliva at levels sufficient to hydrolyze ester-containing composites important to restorative denstistry. The study also seeks to ask if the active sites of CE and PCE with respect to composite could be inhibited. Photo-polymerized model composite resin was incubated in PCE and CE solutions, in the presence and absence of a specific esterase inhibitor, phenylmethylsulfonyl fluoride (PMSF). Incubation solutions were analyzed for resin degradation products by high-performance liquid chromatography (HPLC), UV spectroscopy, and mass spectrometry. Saliva was found to contain both hydrolase activities at levels that could degrade composite resins. PMSF inhibited the composite degradation, indicating a material hydrolysis mechanism similar to the enzymes' common function.

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Origin of Esterases in Human Whole Saliva

Cited by 26 publications

References 17 publications

Effect of human saliva on surface degradation of composite resins

Effect of human saliva on surface degradation of composite resins

Effect of salivary esterase on the integrity and fracture toughness of the dentin‐resin interface

Salivary Esterase Activity and Its Association with the Biodegradation of Dental Composites

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