Origin of marrow stromal cells and haemopoietic chimaerism following bone marrow transplantation determined by in situ hybridisation

Abstract

Summary The origin and cell lineage of stromal cells in the bone marrow is uncertain. Whether a common stem cell exists for both haemopoietic and stromal cells or whether these cell lines arise from distinct stem cells is unknown. Using in situ hybridisation for detection of the Y chromosome, we have examined histological sections of bone marrow from seven patients who received marrow transplants from HLA-matched donors of the opposite sex. Stromal cells (adipocytes, fibroblasts, endothelial cells, osteoblasts and osteocytes) were identified in these recipients as being of host origin. This result is consistent with the concept of a distinct origin and separate cell lineage for cells of the haemopoietic and stromal systems. It also shows that engraftment of marrow stromal cell precursors does not occur and that host stromal cells survive conditioning regimens for marrow transplantation. With the exception of one case, with a markedly hypocellular marrow, mixed chimaerism was seen in haemopoietic cells, indicating that this is not a rare event after marrow transplantation.

Persistence of recipient-type endothelium after allogeneic hematopoietic stem cell transplantation

“…So far, endothelial cell chimerism has not been analyzed after ABO-incompatible HSCT, but it has been described after gender-mismatched HSCT. Two earlier studies, which did not find donor-type endothelial cells in the stromal constituents of bone marrow up to 3 years after HSCT, 29,30 were contrasted by several reports showing endothelial cell chimerism in skin, gut, heart and bone marrow after gender-mismatched HSCT. [14][15][16][17][18]31,32 All these studies used XY in situ hybridization for the detection of donor-derived cells.…”

Human Mesenchymal Stem Cells Are Not of Donor Origin in Patients with Severe Aplastic Anemia Who Underwent Sex-Mismatched Allogeneic Bone Marrow Transplant

“…Some controversy exists in the literature as to whether stromal elements contribute to donor chimerism after an allogeneic transplant in patients (19,(27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39). In this study, we did not analyze stromal donor contribution, but rather focused on the stromal progenitors, the MSC, which were isolated and expanded according to Caplan et al (9,(40)(41)(42)(43)(44).…”

Marrow stromal cells as a source of progenitor cells for nonhematopoietic tissues in transgenic mice with a phenotype of osteogenesis imperfecta

“…Also, after implantation in chambers with porous membranes or on ceramic cubes, the cells form fibrous tissue, cartilage, and bone in vivo. Some reports (19)(20)(21)(22)(23)(24) suggested that MSCs do not engraft in marrow after systemic infusion, but other reports described successful marrow engraftment (2,(25)(26)(27)(28)(29)(30). Many of the negative experiments may be explained by species differences in the animal models, the conditions used for marrow ablation, the number of cells infused, the protocols used to prepare the cells, or the markers used to identify the donor cells (10).…”