Origin of periendothelial cells in microvessels derived from human microvascular endothelial cells

Njauw, Ching‐Ni Jenny; Hu, Yuan; Zheng, Lei; Yao, Min; Martins‐Green, Manuela

doi:10.1016/j.biocel.2007.10.012

Cited by 8 publications

(10 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, pericytes cover capillaries and other microvessels, being often organised in a discontinuous cell layer around the endothelial cell tube (Gaengel et al, 2009). Newly formed vasculature would regress without the support of these periendothelial cells (Conway et al, 2001), which contribute to stabilise blood vessels through direct physical contact (Bergers and Song, 2005), ECM deposition (Njauw et al, 2008) and growth factors release, as vascular endothelial growth factor (VEGF) and Ang-1 (Bergers and Song, 2005;Njauw et al, 2008;Carmeliet and Jain, 2011).…”

Section: Mural Cellsmentioning

confidence: 99%

“…Cellular strategies for vascularisation is mainly composed of fibroblasts and associated ECM components (Njauw et al, 2008). Fibroblasts secrete potent angiogenic factors, like VEGF and fibroblast growth factor (FGF)-2, as well as matrix metalloproteinase (MMP)-2 and MMP-9 (Berthod et al, 2006), which in turn act by releasing pro-angiogenic peptides.…”

Section: Fibroblastsmentioning

confidence: 99%

“…Fibroblasts secrete potent angiogenic factors, like VEGF and fibroblast growth factor (FGF)-2, as well as matrix metalloproteinase (MMP)-2 and MMP-9 (Berthod et al, 2006), which in turn act by releasing pro-angiogenic peptides. Hence, it is possible that fibroblasts act as peri-endothelial progenitors in vivo (Njauw et al, 2008), supporting EC survival and migration, as well as modulating the expansion of capillary-like networks, particularly in vitro (Kunz-Schughart et al, 2006). Moreover, since fibroblasts generate a scaffold for other cells through matrix deposition, they may alter the mechanical extracellular microenvironment, thus regulating the vascularisation processes (Hurley et al, 2010).…”

Section: Fibroblastsmentioning

confidence: 99%

See 2 more Smart Citations

Cellular strategies to promote vascularisation in tissue engineering applications

Costa‐Almeida

Granja²,

Soares³

et al. 2014

eCM

View full text Add to dashboard Cite

Vascularisation is considered to be one of the greatest challenges in tissue engineering. Different strategies exist but cell-based approaches have emerged as a promising therapy to achieve successful vascularisation. The use of endothelial cells to engineer vascularised tissues has been extensively investigated. This field of research has evolved with the discovery of endothelial progenitor cells, a subpopulation with a high regenerative potential. However, the survival of endothelial cell populations alone seems to be impaired. To overcome this problem, co-culture systems, involving supporting cells, like mural cells, fibroblasts, or more tissue-specific cells have been developed. Endothelial cells benefit from the extracellular matrix components and growth factors produced by the supporting cells, which results in neovessel stabilisation and maturation. The use of endothelial progenitor cells in co-culture systems appears to be a promising strategy to promote vascularisation in approaches of increasing complexity. Herein, the authors provide an overview of the cellular strategies that can be used for increasing vascularisation in tissue engineering and regeneration.

show abstract

Section: Mural Cellsmentioning

confidence: 99%

Section: Fibroblastsmentioning

confidence: 99%

Section: Fibroblastsmentioning

confidence: 99%

See 1 more Smart Citation

Cellular strategies to promote vascularisation in tissue engineering applications

Costa‐Almeida

Granja²,

Soares³

et al. 2014

eCM

View full text Add to dashboard Cite

show abstract

“…25 Fibroblast-derived proteins, including growth factors and matrix proteins, have been shown to modulate EC sprouting and the expansion of capillary-like networks in vitro, [26][27][28] contributing to the role of fibroblasts as periendothelial cells in vivo. 29 Thus, the hypothesis underlying herein is that, when cocultured with OECs or mature ECs, different types of fibroblasts will exert distinct influences in the assembly of capillary-like structures. Experiments using cocultures of ECs with two types of human dermal fibroblasts (HDF) in direct contact were performed; the ECM produced over time in these coculture systems was characterized and their ability to induce/support the formation of vascular-like networks was investigated.…”

mentioning

confidence: 99%

Fibroblast-Endothelial Partners for Vascularization Strategies in Tissue Engineering

Costa‐Almeida

Gómez-Lázaro

Ramalho

et al. 2015

Tissue Engineering Part A

View full text Add to dashboard Cite

Cell-based approaches have emerged as a promising therapy to achieve successful vascularization in tissue engineering. Since fibroblasts activation and migration is required for physiological events relying on angiogenesis, we hypothesize herein that different fibroblasts exhibit distinct capacity to promote capillary-like structures assembly, by mature and progenitor endothelial cells (ECs). Outgrowth endothelial cells (OECs) were isolated from human umbilical cord blood samples and characterized by immunofluorescence and imaging flow cytometry for endothelial markers. Coculture systems were established using either human umbilical vein ECs (HUVECs) or OECs with fibroblasts, being evaluated at 7, 14, and 21 days of culture. Two types of human dermal fibroblasts (HDF) were used, namely neonatal human foreskin fibroblasts-1 (HFF-1) and juvenile HDF. OECs expressed EC markers and formed capillary-like structures. HFF-1 exhibited higher expression of transglutaminase-2, while HDF exhibited a higher expression of a-smooth muscle actin (a-SMA) and podoplanin, which were not observed for HFF-1. Formation of capillary-like structures was only observed in cocultures with HDF and not with HFF-1. No significant differences were found between HDF and OECs or HUVECs cocultures. These findings suggest that HDF is a preferential cell source for promoting vascularization, either using mature or progenitor ECs, probably due to their higher expression of a-SMA and podoplanin, and increased synthesis of extracellular matrix. This work opens new research possibilities regarding the use of specific fibroblast populations cocultured with ECs, as efficient partners for vascular development in regenerative medicine strategies.

show abstract

“…In fact, the effect of fibroblasts on PMVECs is also important in the process of pulmonary fibrosis (Njauw et al, 2008;Oberringer et al, 2008;Kuruvilla and Kartha, 2009). As previously reported, fibroblasts can stimulate angiogenesis and stabilize the neovascular endothelium, which in turn promotes the morphological and functional transformation of fibroblasts into myofibroblasts (Villaschi and Nicosia, 1994) and provides critical support for the differentiation of vascular endothelial cells (Ingber and Folkman, 1989;Black et al, 1998;L'Heureux et al, 1998;Hudon et al, 2003;Iivanainen et al, 2003;Jain, 2003).…”

Section: Discussionmentioning

confidence: 72%

Pulmonary microvascular endothelial cells from bleomycin‐induced rats promote the transformation and collagen synthesis of fibroblasts

Yin

Nan

Zhang³

et al. 2011

Journal Cellular Physiology

View full text Add to dashboard Cite

Accumulation and activation of myofibroblasts are the hallmark of progressive pulmonary fibrosis, and the resident fibroblasts are the major source of myofibroblasts. However, the key factors involved in the transformation of fibroblasts are unknown. Pulmonary microvascular endothelial cells (PMVECs), major effector cells against pathogenesis in early stages of the disease, can secrete cytokines to induce the differentiation of mesenchymal cells. We speculated that PMVECs could secrete pro-fibrotic cytokines and promote the transformation of fibroblasts into myofibroblasts. Accordingly, we established a co-culture system with PMVECs and fibroblasts to examine the specific transformation and collagen synthesis of the co-cultured fibroblasts by FACS and Western blot, prior to and after treatment with neutralizing antibodies against transforming growth factor-beta1 (TGF-β1) and connective tissue growth factor (CTGF). We also analyzed expression of TGF-β1 and CTGF in PMVECs. The synthesis and secretion of TGF-β1 and CTGF protein were up-regulated in PMVECs isolated from bleomycin (BLM)-treated rats, most prominently at 7 days post-instillation. We showed that the PMVECs isolated from BLM-induced rats could induce the transformation of normal fibroblasts and their secretion of collagen I, which was inhibited by both neutralizing anti-TGF-β1 and anti-CTGF antibodies. Therefore, up-regulation of TGF-β1 and CTGF in PMVECs plays an important role in activation, transformation, and collagen synthesis of fibroblasts; in particular, these effects in PMVECs are likely to be the key factors for activation and stimulation of static fibroblasts in lung interstitium in early stages of pulmonary fibrosis disease.

show abstract

Origin of periendothelial cells in microvessels derived from human microvascular endothelial cells

Cited by 8 publications

References 35 publications

Cellular strategies to promote vascularisation in tissue engineering applications

Cellular strategies to promote vascularisation in tissue engineering applications

Fibroblast-Endothelial Partners for Vascularization Strategies in Tissue Engineering

Pulmonary microvascular endothelial cells from bleomycin‐induced rats promote the transformation and collagen synthesis of fibroblasts

Contact Info

Product

Resources

About