Origin of system peaks in single-column ion chromatography of inorganic anions using high pH borate—gluconate buffers and conductivity detection

Erkelens, Corrie; Billiet, H.A.H.; Galan, L. de; Leer, E. W. B. De

doi:10.1016/s0021-9673(01)86837-0

Cited by 27 publications

(6 citation statements)

References 9 publications

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“…The suspensions were agitated at 250 rpm for 1 h at room temperature. Aliquots of the solution‐phase were centrifuged at 7000 × g , and the supernatant P concentration determined by a high‐performance liquid chromatographic (HPLC) method for anionic species (Erkelens et al, 1986). A Waters 2690 LC system, equipped with UV (Model PDA 996) and electrical conductivity (Model W432) detectors (Waters Corp., Milford, MA) was used.…”

Section: Methodsmentioning

confidence: 99%

Ligands and Phytase Hydrolysis of Organic Phosphorus in Soils Amended with Dairy Manure

Dao

2004

Agronomy Journal

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tion of IP6 and other IPs in dairy manure are attributed to the protective effect of IP6 complexation with polyva-Information about orthophosphate and organic phosphorus (P) lent cations that reduces dephosphorylation. As a result, desorption in soil amended with animal manure is needed because a build-up of P following repeated manure applications poses risks of substantial amounts of dietary IP6-P of feed grains are offsite dissolved P discharges. An enzymatic hydrolysis and soil P excreted, contributing to water pollution rather than desorption study was conducted to develop a novel ligand-based phyanimal growth and development. tase-hydrolyzable P (PHP) method and quantify water-extractableIn soils amended with animal manure, IP6 and other uncomplexed and complexed P forms (i.e., bioactive P) in Unicorn organic P are added to the soil P pool, in addition to and Christiana soils (Typic Hapludults). Extractability of inorganic inorganic PO 4 -P. The need for detailed organic P char-P with ligands alone (LEP) increased from 2 and 22 mg kg Ϫ1 (0.3 and acterization studies is critical as orthophosphate mono-3.1 Ϯ 0.16% total P) up to sevenfold with increasing ligand (LIGND) and diesters have been observed to move through soils, concentrations in the order of CDTA ϭ EDTA Ͼ DTPA Ͼ Ͼ oxalate. posing risks of water quality degradation via soil subsur-The LIGNDs nullified the benefits of using an anion sink in soil P face transport (Wild and Oke, 1966; Gerritse and Ekdesorption measurements. Soil organic P was hydrolyzed in situ by Aspergillus ficuum (Reichardt) Henn. phytase EC 3.1.3.8, corroborat-steen, 1978; Chardon et al., 1997). myo-Inositol hexakis ing the presence of myo-inositol phosphomonoesters including IP6.dihydrogenphosphate and lower phosphomonoesters of Adding CDTA and EDTA increased phytases' efficacy and that of inositol are the most abundant identifiable organic P the PHP assay for measuring previously inaccessible complexed orcompounds, and may make up to 50% of total soil organic P; soil PHP increased from 2.3 and 6.4% to peak at 32.0 Ϯ 0.7 ganic P (Cosgrove, 1962;Wild and Oke, 1966). The and up to 40.8 Ϯ 0.2% of total P in Unicorn and Christiana soils, phosphomonesters have been identified by 31 P nuclear respectively. In long-term manure-amended Christiana silt loam under magnetic resonance (NMR) spectroscopy, using alkaorchardgrass-red clover stands, the LIGND-PHP method showed line extractants such as 0.5 M NaOH or 0.25 M NaOHthat repeated manure applications resulted in soil storage of unextract-0.05 M Na 2 EDTA (Newman and Tate, 1980; Cade-Menun able complexed PHP and a buildup of inorganic LEP. Accumulation

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Section: Methodsmentioning

confidence: 99%

Ligands and Phytase Hydrolysis of Organic Phosphorus in Soils Amended with Dairy Manure

Dao

2004

Agronomy Journal

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“…The supernatant was filtered through membranes with 0.45‐μm openings. Aliquots of the supernatant were re‐acidified to pH 2 before P analysis to dissolve all forms of PO 4 –P based on a HPLC method for anionic species (Erkelens et al, 1986). Dissolved P concentrations of suspension aliquots were determined chromatographically using a Waters (Milford, MA) 2690 LC system, equipped with UV (Model PDA 996) and electrical conductivity (Model W432) detectors.…”

Section: Methodsmentioning

confidence: 99%

Organic Ligand Effects on Enzymatic Dephosphorylation of myo‐Inositol Hexakis Dihydrogenphosphate in Dairy Wastewater

Dao

2004

J of Env Quality

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Animal manure contains partially digested feed fiber and grains where phosphorus (P) is bound in organic compounds that include myo-inositol 1,2,3,5/4,6-hexakis dihydrogenphosphate or phytic acid (IP6). Information is needed on the effects of other (non-IP6) organic ligands (LIGND) on the enzymatic dephosphorylation of IP6, which is a potential source of dissolved orthophosphate P (PO4-P) in the soil-manure-water system. The effects of 1,2-cyclohexane diamino-tetraacetate (CDTA), diethylenetriamine-N,N,N',N'',N''-pentaacetate (DTPA), ethylenediamine-N,N,N',N'-tetraacetate (EDTA), oxalate (OXA), and phthalate (PHTH) and LIGND to IP6 molar ratio and charge concentration ratio on IP6 dephosphorylation were studied to determine controlling mechanisms of IP6 persistence in manure. Solution PO4-P concentrations were analyzed by ion chromatography as the phosphomolybdate-ascorbic acid method partly includes IP6-P. Uncomplexed IP6 dephosphorylation by Aspergillus ficuum (Reichardt) Henn. phytase EC 3.1.3.8 at pH 4.5 and 6 is unaffected by the presence of LIGNDs. As the concentrations of Ca2+, Al3+, or Fe3+ increase, dephosphorylation is reduced. Their inhibitory effect lessens in the presence of LIGNDs, in the following order: CDTA = EDTA > DTPA >> OXA > or = PHTH. Whether CDTA or EDTA is the most effective LIGND depends upon the acidity of the suspension and LIGND charge concentration, reducing the inhibitory effect of polyvalent counterions to the point of promoting the hydrolysis of a manure phytase-hydrolyzable phosphorus (PHP) fraction that is otherwise unavailable. Therefore, ligand-induced changes increase the mobilization and dephosphorylation of complexed organic P, above and beyond the simple dissolution of inorganic phosphates. An analytical method for potentially bioavailable PHP in animal manure should include a LIGND as extracting reagent. Also, potential LIGNDs in an organic carbon-rich dairy wastewater may increase the release of PHP and environmental dispersion of PO4-P.

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“…It is mainly important for biological and pharmaceutical samples, polymers, and food samples [149,150].…”

Section: Sample Preparation For Ion Chromatographymentioning

confidence: 99%

Principles and Applications of Ion Chromatography

Michalski¹

2016

Application of IC‐MS and IC‐ICP‐MS in Environmental Research

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Origin of system peaks in single-column ion chromatography of inorganic anions using high pH borate—gluconate buffers and conductivity detection

Cited by 27 publications

References 9 publications

Ligands and Phytase Hydrolysis of Organic Phosphorus in Soils Amended with Dairy Manure

Ligands and Phytase Hydrolysis of Organic Phosphorus in Soils Amended with Dairy Manure

Organic Ligand Effects on Enzymatic Dephosphorylation of myo‐Inositol Hexakis Dihydrogenphosphate in Dairy Wastewater

Principles and Applications of Ion Chromatography

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