Abstract.We examined the longitudinal effects of 3 experimental floods on seston, periphyton, and macroinvertebrates in a regulated river downstream from a large reservoir in the Swiss Alps. In addition, suspended organic sediments, in conjunction with conductivity and turbidity, were measured during each flood. Three study reaches were selected downstream from the reservoir: an Upper site ca 150 m, a Middle site at 1.2 km, and a Lower site at 2.3 km from the dam. The Upper site was located upstream of any significant source of sediments. No flood effect was observed on water temperature, although river temperatures and diel amplitudes increased progressively downstream from the dam. Most organic sediments were mobilized and transported within the first few hours of each flood, with the pulse in sediments typically occuring later (after peak discharge) and being more prolonged at the Lower site. Turbidity generally decreased Aquat. Sci. 65 (2003) Dübendorf, 2003 Aquatic Sciences during the flood season, perhaps in response to lower amounts of fines and reductions in periphyton biomass. Seston concentrations (as chlorophyll a and AFDM) were lowest at the Upper site, and typically decreased following each flood. The flood response in seston was lowest at the Upper site and more pronounced at the Middle and Lower sites. Periphyton biomass also was lowest at the Upper site, and the flood effects were significant only in the Middle and Lower sites. In contrast, macroinvertebrate densities were similar between the Upper and Lower sites, and both sites exhibited a similar response to the floods. These results indicate a longitudinal response of river benthos to flooding below the reservoir that partly reflects the examined benthic constituent (periphyton or zoobenthos) and the longitudinal differences in the degree and type (e. g., flow in combination with sediment mobility and scouring) of disturbance.223 -231 1015-1621/03/030223-09 DOI 10.1007/s00027-003-0662-9 © EAWAG,