ORIGINAL RESEARCH: Electrophoretic fractionation of creatine kinase isoenzymes and macroenzymes in clinically healthy dogs and cats and preliminary evaluation in central neurologic disease

Paltrinieri, Saverio; Cazzaniga, Stefano; Cunha, Nazaré Pinto da; Giordano, Alessia

doi:10.1111/j.1939-165x.2010.00242.x

Cited by 10 publications

(35 citation statements)

References 20 publications

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“…Activities of enzymes released from affected skeletal muscle such as CK and LDH can also increase . The activity of the brain isoenzyme of CK can be increased in the presence of neurologic signs due to Leishmania‐ related cerebrovascular alterations, while increases in the myocardial isoenzyme of CK can occur with cardiomyopathy. In such latter cases, increased troponin I has also been reported .…”

Section: Laboratory Abnormalities That May Support or Confirm Leishmamentioning

confidence: 99%

Laboratory tests for diagnosing and monitoring canine leishmaniasis

Paltrinieri

Gradoni

Roura

et al. 2016

Veterinary Clinical Pathol

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Although several reviews on canine leishmaniasis have been published, none thoroughly described clinicopathologic abnormalities and their clinical usefulness. The aim of this review was to provide information concerning current diagnostic tests relevant for clinical pathologists and from a practical perspective. Specifically, in canine leishmaniasis, nonregenerative normocytic normochromic anemia, thrombocytopenia, or leukogram changes may be present. Clinical chemistry and urinalysis may indicate renal dysfunction (azotemia, decreased urine specific gravity, proteinuria) and an inflammatory/immune response (increased acute phase proteins [APP] or α - and/or γ-globulins). Although a potential gammopathy is usually polyclonal, it may also appear oligo- or monoclonal, especially in dogs coinfected by other vector-borne pathogens. When lesions are accessible to fine-needle aspiration (lymphoadenomegaly, nodular lesions, joint swelling), cytology is strongly advised, as the presence of Leishmania amastigotes in a pattern of pyogranulomatous inflammation or lymphoplasmacytic hyperplasia is diagnostic. If the cytologic pattern is inconclusive, the parasite should be identified by histology/immunohistochemistry or PCR on surgical biopsies. Alternatively, cytology and PCR may be performed on bone marrow samples where amastigotes, along with erythroid hypoplasia, myeloid hyperplasia, plasmacytosis, or secondary dysmyelopoiesis can be observed. Dogs with overt leishmaniasis generally have high antibody titers, while low titers predominate in immunologically resistant infected dogs or in exposed dogs with no parasite confirmation. Quantitative serology is recommended in clinically suspect dogs as high-titer antibodies titers may confirm the clinical diagnosis. In confirmed and treated dogs, renal function and inflammatory/immune response variables should be periodically monitored.

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Section: Laboratory Abnormalities That May Support or Confirm Leishmamentioning

confidence: 99%

Laboratory tests for diagnosing and monitoring canine leishmaniasis

Paltrinieri

Gradoni

Roura

et al. 2016

Veterinary Clinical Pathol

Self Cite

149

232

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“…Creatine kinase is composed of 2 monomers, B and M . Combinations of these monomers generate the 3 isoenzymes CK‐BB, CK‐MB, and CK‐MM, which are found predominantly in brain, heart, and skeletal muscle, respectively . A small amount of CK‐BB may also be produced in intestine and spleen .…”

Section: Introductionmentioning

confidence: 99%

Serum creatine kinase isoenzymes and macroenzymes in dogs with different neurologic diseases

Paltrinieri

Pintore²,

Balducci³

et al. 2017

Veterinary Clinical Pathol

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“…Recent publications in Veterinary Clinical Pathology ( VCP ) have demonstrated the use of this technique in very different animal species and in a variety of protein abnormalities 8–11 . However, cellulose acetate and agarose gel electrophoresis are still used as reported in previous papers in VCP 12–21 . Currently, most papers describe CZE in comparison with cellulose acetate or agarose systems.…”

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confidence: 99%

“…[8][9][10][11] However, cellulose acetate and agarose gel electrophoresis are still used as reported in previous papers in VCP. [12][13][14][15][16][17][18][19][20][21] Currently, most papers describe CZE in comparison with cellulose acetate or agarose systems. Because the resolution of CZE can be much higher than that of the other systems and proteins behave differently, reference values must be calculated again.…”

mentioning

confidence: 99%