Origins of cholinergic inputs to the cell bodies of intestinofugal neurons in the guinea pig distal colon

Lomax, Alan E.; Zhang, Jonathan Y.; Furness, John B.

doi:10.1002/(sici)1096-9861(20000124)416:4<451::aid-cne3>3.0.co;2-e

Cited by 32 publications

(30 citation statements)

References 47 publications

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“…The results of the present study have shown that DMPP-responsive viscerofugal neurons in guinea-pig colon have uni-axonal cell bodies with ''simple'' or Dogiel type I morphology. These findings are consistent with previous reports in the guinea pig (Kuramoto and Furness, 1989;Furness et al, 1990;Furness, 1992, 1993;Sharkey et al, 1998;Tassicker et al, 1999;Lomax et al, 2000;Olsson et al, 2004) and in a recent study of organ-cultured preparations in which extrinsic neurons had degenerated (Hibberd et al, in press). …”

Section: Morphological Identification Of Afferent Neuronssupporting

confidence: 86%

Identification and mechanosensitivity of viscerofugal neurons

et al. 2012

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Section: Morphological Identification Of Afferent Neuronssupporting

confidence: 86%

Identification and mechanosensitivity of viscerofugal neurons

et al. 2012

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“…Extrinsic denervation was accomplished using the surgical technique described by Messenger and Furness (1992) and Lomax et al (2000). Before surgery, guinea pigs were sedated with Diazepam (5 mg/kg, i.p.…”

Section: Methodsmentioning

confidence: 99%

Enteric Glia Are Targets of the Sympathetic Innervation of the Myenteric Plexus in the Guinea Pig Distal Colon

Gulbransen¹,

Bains²,

Sharkey³

2010

J. Neurosci.

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Astrocytes respond to synaptic activity in the CNS. Astrocytic responses are synapse specific and precisely regulate synaptic activity. Glia in the peripheral nervous system also respond to neuronal activity, but it is unknown whether glial responses are synapse specific. We addressed this issue by examining the activation of enteric glia by distinct neuronal subpopulations in the enteric nervous system. Enteric glia are unique peripheral glia that surround enteric neurons and respond to neuronally released ATP with increases in intracellular calcium ( ] i responses is ATP, suggesting that sympathetic fibers release ATP to activate enteric glia. Therefore, enteric glia discern activity in adjacent synaptic pathways and selectively respond to sympathetic activation.

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“…By examining the colocalisation patterns of these neurochemical markers, together with ChAT and VAChT, in myenteric cell bodies and varicosities, four classes of descending interneurons have been identified in the present work. There is a class of cholinergic descending interneurons that is immunoreactive for VIP and often GRP, NOS and calbindin, as previously described (Lomax et al 2000). On the basis of cell-body staining observed in the present study, another class of cholinergic descending interneuron is 5-HT-IR, ChAT-IR, CGRP-IR, calbindin-IR and sometimes calretinin-IR.…”

Section: Descending Interneuronsmentioning

confidence: 98%

“…Although the patterns of motor activity of the external musculature and the mucosa of the colon differ from those of the small intestine Christensen 1994;Cooke and Reddix 1994), the only marked difference in neural circuitry between the two regions appears to be that of the myenteric interneurons; both the IPANs and the motor neurons to the muscle and the mucosa appear to be homologous to those in the small intestine. In the small intestine, three classes of descending and one class of ascending interneuron have been identified (Costa et al 1996;Furness et al 2000), whereas in the colon, four classes of descending interneuron and three classes of ascending interneuron have been defined on the basis of chemical coding (Lomax et al 2000;present work). Physiological, pharmacological and immunohistochemical studies have shown that the external musculature of every region of the GI tract of all species examined receives inhibitory and excitatory innervation from enteric motor neurons (see Furness et al 1995).…”

Section: Comparison With the Guinea-pig Small Intestinementioning

confidence: 99%

Neurochemical classification of enteric neurons in the guinea-pig distal colon

Lomax

Furness

2000

Cell and Tissue Research

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240

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Previous studies have identified the chemistries, shapes, projections and electrophysiological characteristics of several populations of neurons in the distal colon of the guinea-pig but it is unknown how these characteristics correlate to define the classes of neurons present. We have used double-label immunohistochemical techniques to identify neurochemically distinct subgroups of enteric neurons in this region. On the basis of colocalisation of neurochemical markers and knowledge gained from previous studies of neural projections, 17 classes of neurons were identified. The myenteric plexus contained the cell bodies of 13 distinct types of neurons. Four classes of descending interneurons and three classes of ascending interneurons were identified, together with inhibitory and excitatory motor neurons to both the circular and longitudinal muscle layers. Dogiel type II neurons, which are presumed to be intrinsic primary afferent neurons, were located in myenteric and submucosal ganglia; they were all immunoreactive for choline acetyltransferase and often calbindin and tachykinins. Three classes of secretomotor neurons with cell bodies in submucosal ganglia were defined. Two of these classes were immunoreactive for choline acetyltransferase and the other class was immunoreactive for both vasoactive intestinal peptide and nitric oxide synthase. Some of the secretomotor neurons probably also have a vasomotor function. The neural subtypes defined in the present study are similar in many respects to those found in the small intestine, although differences are evident, especially in populations of interneurons. These differences presumably reflect the differing physiological roles of the two intestinal regions.

show abstract

Origins of cholinergic inputs to the cell bodies of intestinofugal neurons in the guinea pig distal colon

Cited by 32 publications

References 47 publications

Identification and mechanosensitivity of viscerofugal neurons

Identification and mechanosensitivity of viscerofugal neurons

Enteric Glia Are Targets of the Sympathetic Innervation of the Myenteric Plexus in the Guinea Pig Distal Colon

Neurochemical classification of enteric neurons in the guinea-pig distal colon

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