Origins of positive potential difference of frog gastric mucosa in Cl--free solutions

Schwartz, Manuel; Chu, Teh-Ching; Carrasquer, Gaspar; Rehm, Warren S.; Holloman, Thomas L.

doi:10.1152/ajpgi.1981.240.4.g267

Cited by 2 publications

(2 citation statements)

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“…Since the active transport of sodi um is relatively fast compared with the passive diffusion of chloride ions, the net result is a greater number of posi tive charges on the serosal surface and a greater number of negative charges on the luminal surface. Further, since epithelial types inherently differ with respect to rate and type of ions transported and/or the magnitude and type of epithelial resistance to passive ion movements, each tis sue has its own characteristic PD [10], PD originates through an energy-requiring process and has been shown to decline when oxygen availability is reduced [11][12][13][14],…”

Section: Origin Of Pdmentioning

confidence: 99%

Transmucosal Potential Difference as an Index of Esophageal Mucosal Integrity

Scarpignato

Micali²,

Galmiche³

1995

Digestion

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All the epithelia lining the gastrointestinal (GI) tract, including that of the esophagus, exhibit a transmucosal electrical potential difference (PD). The luminal surface of the GI mucosa is indeed electrically negative when compared with the serosal one. Although it was initially felt that the body of the esophagus exhibits a PD near 0 or slightly positive, recent studies, using parenteral reference electrodes, have shown a negative PD of around -15 mV. Measurement of esophageal PD has been mainly used to locate both the lower and the upper esophageal sphincters but very rarely to evaluate esophageal mucosal integrity in clinical settings, most probably due to the difficulties encountered during measurement of mucosal PD. Reliable techniques to measure esophageal PD simultaneously with esophageal pressure or mucosal pH are now available. Application of these recently developed methodologies showed that measurement of esophageal PD during either manometry or endoscopy provides meaningful information about mucosal integrity. Indeed, tissue injury, either neoplastic or inflammatory, usually results in a less negative PD. In contrast, an abnormally high negative PD is very often observed in patients with columnar-lined lower esophagus. In patients with microscopic reflux esophagitis, PD exhibits less negative values which are significantly correlated with the degree of the mucosal damage. Normalization of the altered PD after either medical or surgical treatment makes it an additional parameter to evaluate the effect of a given therapy.

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Section: Origin Of Pdmentioning

confidence: 99%

Transmucosal Potential Difference as an Index of Esophageal Mucosal Integrity

Scarpignato

Micali²,

Galmiche³

1995

Digestion

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“…The luminal surface of the gastric mucosa is electrically negative when compared with the serosal surface [1], There is a prominent lumen-negative transmucosal potential dif ference in the stomach (gastric potential dif ference, GPD) [2], Even if different ions are implicated in the generation of GPD, its principal source seems to be the active transport of chloride ions from the intracellular space against both concentration and electrochemical gradients [2,3], GPD originates through an energyrequiring process and has been shown to decline when oxygen availability is reduced [4][5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Measurement and Recording of Gastric Potential Difference and Intragastric pH in Man

Scarpignato

Galmiche

1990

Dig Dis

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Gastric potential difference (GPD) is used increasingly as an index of mucosal integrity. The technique for GPD measurement currently employed is however laborious due to the preparation and check of KCl-agarose bridges prior to each experiment. In addition, calculations – usually handmade – are time-consuming and inaccurate. Therefore, a new apparatus allowing continuous recording of basal and drug-induced changes in GPD values was developed. A commercially available 24-hour pH monitor (Proxima Light®^, Synectics Medical) was modified in order to allow GPD measurement and recording with subsequent elaboration of data. Furthermore, simultaneous measurement of GPD and intragastric pH through the use of a single stomach probe was made possible. The new method was checked towards the classical PD measuring system employing KCl-agarose bridges. The readings obtained with both systems, either in basal conditions or after aspirin, correlated in a highly significant way. Readings proved to be quite stable with a variation, for 150 consecutive values, of less than 2 mV. With this system the effect of pH modifications, obtained through H₂ receptor stimulation and inhibition, on the behaviour of GPD values was investigated in healthy volunteers. Analysis of paired data obtained from these experiments showed an inverse relationship between these two parameters. This suggests that GPD and pH should be measured together, especially during experiments aiming to study drug effects on gastric mucosa. Although the apparatus described was developed for PD measurement and recording in the stomach, it can be used easily for PD measurement across the mucosa of the entire digestive tract, for example in the esophagus, colon or rectum.

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Origins of positive potential difference of frog gastric mucosa in Cl--free solutions

Cited by 2 publications

References 0 publications

Transmucosal Potential Difference as an Index of Esophageal Mucosal Integrity

Transmucosal Potential Difference as an Index of Esophageal Mucosal Integrity

Simultaneous Measurement and Recording of Gastric Potential Difference and Intragastric pH in Man

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