Origins of rice cytoplasmic male sterility genes

Charlesworth, Deborah

doi:10.1038/cr.2016.158

Cited by 10 publications

(4 citation statements)

References 14 publications

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“…On the other hand, the monogenetic male-sterile gene including the photoperiod-sensitive male sterile (PGMS) and thermosensitive male sterile are useful to facilitate the production of F 1 seeds [70] or the intercrossing phase of recurrent selection. Several genes for PGMS and thermosensitive male sterile were mapped or isolated [71][72][73]. However, their monogenetic inheritance and the expression of male sterility are certainly distinguished from the complementary sterility caused by the two genes d60 and gal.…”

Section: Discussionmentioning

confidence: 99%

The Gametic Non-Lethal Gene Gal on Chromosome 5 Is Indispensable for the Transmission of the Co-Induced Semidwarfing Gene d60 in Rice

Tomita

Tanisaka

2019

Biology

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The gametic lethal gene gal in combination with the semidwarfing gene d60 causes complementary lethality in rice. Here, we attempted to ascertain the existence of gal and clarify male gamete abortion caused by d60 and gal. Through the F2 to F4 generations derived from the cross between D60gal-homozygous and d60Gal-homozygous, progenies of the partial sterile plants (D60d60Galgal) were segregated in a ratio of 1 semidwarf (1 d60d60GalGal):2 tall and quarter sterile (2 D60d60Galgal):6 tall (2 D60d60GalGal:1 D60D60GalGal:2 D60D60Galgal:1 D60D60galgal), which is skewed from the Mendelian ratio of 1 semidwarf:3 tall. However, the F4 generation was derived from fertile and tall heterozygous F2 plants (D60d60GalGal), which were segregated in the Mendelian ratio of 1[semidwarf (d60d60GalGal)]:2[1 semidwarf:3 tall (D60d60GalGal)]:1[tall (D60D60GalGal)]. The backcrossing of D60Gal-homozygous tall F4 plants with Hokuriku 100 resulted in fertile BCF1 and BCF2 segregated in a ratio of 1 semidwarf:3 tall, proving that d60 is inherited as a single recessive gene in the D60d60GalGal genetic background (i.e., in the absence of gal). Further, gal was localized on chromosome 5, which is evident from the deviated segregation of d1 as 1:8 and linkage with simple sequence repeat (SSR) markers. Next-generation sequencing identified the candidate SNP responsible for Gal. In F1 and sterile F2, at the binucleate stage, partial pollen discontinued development. Degraded pollen lost vegetative nuclei, but second pollen mitosis raising two generative nuclei was observed. Thus, our study describes a novel genetic model for a reproductive barrier. This is the first report on such a complementary lethal gene, whose mutation allows the transmission of a co-induced valuable semidwarfing gene d60.

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Section: Discussionmentioning

confidence: 99%

The Gametic Non-Lethal Gene Gal on Chromosome 5 Is Indispensable for the Transmission of the Co-Induced Semidwarfing Gene d60 in Rice

Tomita

Tanisaka

2019

Biology

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“…The origin and mechanisms of CMS are still mysterious (Charlesworth 2017;Chase and Gabay-Laughnan 2004;Hanson and Bentolila 2004). Unusual ORFs determining CMS have been discovered in a wide range of plant species, showing considerable diversity of the proteins involved and the specific phenotypic effects (Chase 2007).…”

Section: Evaluating the Genetic Basis Of The Cms Phenotypementioning

confidence: 99%

Elucidating genomic patterns and recombination events in plant cybrid mitochondria

García

Zubko

et al. 2019

Plant Mol Biol

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Key message Cybrid plant mitochondria undergo homologous recombination, mainly BIR, keep a single allele for each gene, and maintain exclusive sequences of each parent and a single copy of the homologous regions. Abstract The maintenance of a dynamic equilibrium between the mitochondrial and nuclear genomes requires continuous communication and a high level of compatibility between them, so that alterations in one genetic compartment need adjustments in the other. The co-evolution of nuclear and mitochondrial genomes has been poorly studied, even though the consequences and effects of this interaction are highly relevant for human health, as well as for crop improvement programs and for genetic engineering. The mitochondria of plants represent an excellent system to understand the mechanisms of genomic rearrangements, chimeric gene formation, incompatibility between nucleus and cytoplasm, and horizontal gene transfer. We carried out detailed analyses of the mtDNA of a repeated cybrid between the solanaceae Nicotiana tabacum and Hyoscyamus niger. The mtDNA of the cybrid was intermediate between the size of the parental mtDNAs and the sum of them. Noticeably, most of the homologous sequences inherited from both parents were lost. In contrast, the majority of the sequences exclusive of a single parent were maintained. The mitochondrial gene content included a majority of N. tabacum derived genes, but also chimeric, two-parent derived, and H. niger-derived genes in a tobacco nuclear background. Any of these alterations in the gene content could be the cause of CMS in the cybrid. The parental mtDNAs interacted through 28 homologous recombination events and a single case of illegitimate recombination. Three main homologous recombination mechanisms were recognized in the cybrid mitochondria. Break induced replication (BIR) pathway was the most frequent. We propose that BIR could be one of the mechanisms responsible for the loss of the majority of the repeated regions derived from H. niger.

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“…Many studies have revealed that mitochondrial genes are involved in plant male sterility, including atp [ 24 , 25 ], cox [ 24 ], rps [ 24 ], ORF [ 26 , 27 ], and ORF interacted with genes [ 28 ]. The common modes of male sterility were rearrangements of mitochondrial genes and chimeric gene fusion [ 29 , 30 , 31 ]. In addition, the chimeric CMS genes are mostly transcribed together with the upstream or downstream genes, such as ORF79 is co-transcribed with the upstream atp6 gene in rice [ 32 ].…”

Section: Introductionmentioning

confidence: 99%

Chloroplast Genes Are Involved in The Male-Sterility of K-Type CMS in Wheat

Han

Gao

et al. 2022

Genes

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The utilization of crop heterosis can greatly improve crop yield. The sterile line is vital for the heterosis utilization of wheat (Triticum aestivum L.). The chloroplast genomes of two sterile lines and one maintainer were sequenced using second-generation high-throughput technology and assembled. The nonsynonymous mutated genes among the three varieties were identified, the expressed difference was further analyzed by qPCR, and finally, the function of the differentially expressed genes was analyzed by the barley stripe mosaic virus-induced gene silencing (BSMV-VIGS) method. A total of 16 genes containing 31 nonsynonymous mutations between K519A and 519B were identified. There were no base mutations in the protein-encoding genes between K519A and YS3038. The chloroplast genomes of 519B and K519A were closely related to the Triticum genus and Aegilops genus, respectively. The gene expression levels of the six selected genes with nonsynonymous mutation sites for K519A compared to 519B were mostly downregulated at the binucleate and trinucleate stages of pollen development. The seed setting rates of atpB-silenced or ndhH-silenced 519B plants by BSMV-VIGS method were significantly reduced. It can be concluded that atpB and the ndhH are likely to be involved in the reproductive transformation of 519B.

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Origins of rice cytoplasmic male sterility genes

Cited by 10 publications

References 14 publications

The Gametic Non-Lethal Gene Gal on Chromosome 5 Is Indispensable for the Transmission of the Co-Induced Semidwarfing Gene d60 in Rice

The Gametic Non-Lethal Gene Gal on Chromosome 5 Is Indispensable for the Transmission of the Co-Induced Semidwarfing Gene d60 in Rice

Elucidating genomic patterns and recombination events in plant cybrid mitochondria

Chloroplast Genes Are Involved in The Male-Sterility of K-Type CMS in Wheat

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