Origins of the reassortant 2009 pandemic influenza virus through proteotyping with mass spectrometry

Fernandes, Neil; Downard, Kevin M.

doi:10.1002/jms.3310

Cited by 9 publications

(10 citation statements)

References 33 publications

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“…Apart from viral identification, MALDI-TOF MS has also been used in virology for genotyping of JC polyomaviruses ( Bayliss et al, 2010 ), hepatitis B and hepatitis C viruses ( Ilina et al, 2005 ; Ganova-Raeva et al, 2010 ) and for detection of mutations in hepatitis B viruses ( Luan et al, 2009 ). Also, many researchers have demonstrated the application of MALDI-TOF MS for screening of influenza virus subtypes and for tracking epidemiology of influenza viruses ( Schwahn et al, 2009 , 2010 ; Fernandes and Downard, 2014 ). Newer strains of influenza viruses frequently originate by mixing of genetic material of several strains in a common host.…”

Section: Maldi-tof Ms In Virologymentioning

confidence: 99%

MALDI-TOF mass spectrometry: an emerging technology for microbial identification and diagnosis

et al. 2015

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Currently microorganisms are best identified using 16S rRNA and 18S rRNA gene sequencing. However, in recent years matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has emerged as a potential tool for microbial identification and diagnosis. During the MALDI-TOF MS process, microbes are identified using either intact cells or cell extracts. The process is rapid, sensitive, and economical in terms of both labor and costs involved. The technology has been readily imbibed by microbiologists who have reported usage of MALDI-TOF MS for a number of purposes like, microbial identification and strain typing, epidemiological studies, detection of biological warfare agents, detection of water- and food-borne pathogens, detection of antibiotic resistance and detection of blood and urinary tract pathogens etc. The limitation of the technology is that identification of new isolates is possible only if the spectral database contains peptide mass fingerprints of the type strains of specific genera/species/subspecies/strains. This review provides an overview of the status and recent applications of mass spectrometry for microbial identification. It also explores the usefulness of this exciting new technology for diagnosis of diseases caused by bacteria, viruses, and fungi.

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Section: Maldi-tof Ms In Virologymentioning

confidence: 99%

MALDI-TOF mass spectrometry: an emerging technology for microbial identification and diagnosis

et al. 2015

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“…For over two decades, this laboratory has championed, developed, and implemented novel mass spectrometry (MS) based approaches to characterize respiratory and other viruses with fine molecular detail. , Proteotyping approaches have been developed to identify and characterize viral proteins for surveillance, , type, , and subtype , strains; establish their lineage and antigenicity; − and distinguish pandemic , and reassorted strains from seasonal viruses.…”

mentioning

confidence: 99%

“…Our proteotyping studies, − ,− which largely use single signature peptides detected in mass maps to identify, type, and subtype strains should also not be confused with other uses of the term that describe, more generally, the application of protein mass spectrometry in various guises to study microorganisms. These include use of the term for what is better known as biotyping, with and without the need for tandem mass spectrometry.…”

mentioning

confidence: 99%

Detection, Mapping, and Proteotyping of SARS-CoV-2 Coronavirus with High Resolution Mass Spectrometry

Dollman¹,

Griffin²,

Downard³

2020

ACS Infect. Dis.

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A high resolution mass spectrometry approach has been applied for the first time to detect and characterize SARS-CoV-2 coronavirus in cell cultured and nasopharyngeal swab specimens. Peptide ions for three of the most abundant structural viral proteins (membrane, nucleocapid, and spike) are detected and assigned directly, by virtue of the high resolution and mass accuracy within the mass maps of whole virus digests, without the need for tandem mass spectrometry (MS/MS). MALDI-MS based approaches offer high sample throughput and speed, compared with those of LC–MS strategies, and detection limits at some 105 copies, or orders of magnitude less with selected ion monitoring, that compete favorably with conventional reverse transcription polymerase chain reaction (RT-PCR) strategies. The detection of signature peptides unique to SARS-CoV-2 coronavirus over those from the influenza virus allows for its unambiguous detection.

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“…Interestingly, MS allows deciphering virus structural variability (as previously exemplified for bacteriophages) and evolution, through proteotyping and sequence analysis . An alternative may rely on indirect markers of exposition that could be established by specific proteomic approaches aiming at measuring changes in host proteins upon viral infection …”

Section: Emerging and Other Specific Approaches In The Environmental mentioning

confidence: 99%

“…[113,[121][122][123][124][125] An alternative may rely on indirect markers of exposition that could be established by specific proteomic approaches aiming at measuring changes in host proteins upon viral infection. [126][127][128][129]…”

Section: Analysis Of Virusesmentioning

confidence: 99%

Mass spectrometry for the detection of bioterrorism agents: from environmental to clinical applications

Duriez¹,

Armengaud

Fenaille

et al. 2016

J. Mass Spectrom.

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In the current context of international conflicts and localized terrorist actions, there is unfortunately a permanent threat of attacks with unconventional warfare agents. Among these, biological agents such as toxins, microorganisms, and viruses deserve particular attention owing to their ease of production and dissemination. Mass spectrometry (MS)-based techniques for the detection and quantification of biological agents have a decisive role to play for countermeasures in a scenario of biological attacks. The application of MS to every field of both organic and macromolecular species has in recent years been revolutionized by the development of soft ionization techniques (MALDI and ESI), and by the continuous development of MS technologies (high resolution, accurate mass HR/AM instruments, novel analyzers, hybrid configurations). New possibilities have emerged for exquisite specific and sensitive detection of biological warfare agents. MS-based strategies for clinical application can now address a wide range of analytical questions mainly including issues related to the complexity of biological samples and their available volume. Multiplexed toxin detection, discovery of new markers through omics approaches, and identification of untargeted microbiological or of novel molecular targets are examples of applications. In this paper, we will present these technological advances along with the novel perspectives offered by omics approaches to clinical detection and follow-up.

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Origins of the reassortant 2009 pandemic influenza virus through proteotyping with mass spectrometry

Cited by 9 publications

References 33 publications

MALDI-TOF mass spectrometry: an emerging technology for microbial identification and diagnosis

MALDI-TOF mass spectrometry: an emerging technology for microbial identification and diagnosis

Detection, Mapping, and Proteotyping of SARS-CoV-2 Coronavirus with High Resolution Mass Spectrometry

Mass spectrometry for the detection of bioterrorism agents: from environmental to clinical applications

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