OrMKK3 Influences Morphology and Grain Size in Rice

Pan, Ying; Gao, Li; Liang, Yun Tao; Zhao, Yan; Liang, Hai Fu; Chen, Wei Wei; Yang, Xing Hai; Qing, Dong Jin; Gao, Ju; Wu, Hao; Huang, Juan; Zhou, Wei; Huang, Cheng Cui; Dai, Gao Xing; Deng, Guo Fu

doi:10.1007/s12374-020-09290-2

Cited by 7 publications

(7 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…According to our previous study (Pan et al, 2021), OrMKK3 of O. officinalis Wall. ex Watt affects the morphology and grain size of rice.…”

Section: Osmkk3 Regulates Grain Size the Accumulation Of Starch And The Expression Of Other Genes Involved In The Production Of Ricementioning

confidence: 96%

“…A study examining the function of OrMKK3 in Oryza officinalis Wall. ex Watt revealed that the plant height was lower, the grains were shorter, and the number of tillers was higher in plants overexpressing OrMKK3 than wild-type plants of the Nipponbare (Nip) cultivar ( Pan et al, 2021 ). There is thus a need to study the genetic and molecular bases of grain size ( Mao et al, 2010 ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Natural Variation in OsMKK3 Contributes to Grain Size and Chalkiness in Rice

et al. 2021

Self Cite

View full text Add to dashboard Cite

Rice (Oryza sativa L.) is an important staple food crop for more than half of the world’s population. Enhancing the grain quality and yield of rice to meet growing demand remains a major challenge. Here, we show that OsMKK3 encode a MAP kinase kinase that controls grain size and chalkiness by affecting cell proliferation in spikelet hulls. We showed that OsSPL16, GS5, and GIF1 have a substantial effect on the OsMKK3-regulated grain size pathway. OsMKK3 has experienced strong directional selection in indica and japonica. Wild rice accessions contained four OsMKK3 haplotypes, suggesting that the OsMKK3 haplotypes present in cultivated rice likely originated from different wild rice accessions during rice domestication. OsMKK3-Hap1, gs3, and gw8 were polymerized to enhance the grain length. Polymerization of beneficial alleles, such as OsMKK3-Hap1, gs3, gw8, fgr, alk, chalk5, and wx, also improved the quality of hybrid rice. Overall, the results indicated that beneficial OsMKK3 alleles could be used for genomic-assisted breeding for rice cultivar improvement and be polymerized with other beneficial alleles.

show abstract

“…According to our previous study (Pan et al, 2021), OrMKK3 of O. officinalis Wall. ex Watt affects the morphology and grain size of rice.…”

Section: Osmkk3 Regulates Grain Size the Accumulation Of Starch And The Expression Of Other Genes Involved In The Production Of Ricementioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Natural Variation in OsMKK3 Contributes to Grain Size and Chalkiness in Rice

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Although GLW7 can interact with SRS5 to regulate grain size, the exact mechanism remains unclear. Furthermore, the novel gene OrMKK3 affects morphology and grain size, suggesting a relationship between MAPK and BR-responsive pathways with regard to grain development [ 193 ]. Moreover, LARGE2, encoding the HECT E3 UB-ligase OsUPL2, controls the rice panicle size and grain numbers, and large2 mutants showed increased panicle size and number of seeds per panicle [ 194 ].…”

Section: The Role Of Signaling Pathways In Seed Size Regulationmentioning

confidence: 99%

Developing Genetic Engineering Techniques for Control of Seed Size and Yield

Alam

Batool

Huang

et al. 2022

IJMS

View full text Add to dashboard Cite

Many signaling pathways regulate seed size through the development of endosperm and maternal tissues, which ultimately results in a range of variations in seed size or weight. Seed size can be determined through the development of zygotic tissues (endosperm and embryo) and maternal ovules. In addition, in some species such as rice, seed size is largely determined by husk growth. Transcription regulator factors are responsible for enhancing cell growth in the maternal ovule, resulting in seed growth. Phytohormones induce significant effects on entire features of growth and development of plants and also regulate seed size. Moreover, the vegetative parts are the major source of nutrients, including the majority of carbon and nitrogen-containing molecules for the reproductive part to control seed size. There is a need to increase the size of seeds without affecting the number of seeds in plants through conventional breeding programs to improve grain yield. In the past decades, many important genetic factors affecting seed size and yield have been identified and studied. These important factors constitute dynamic regulatory networks governing the seed size in response to environmental stimuli. In this review, we summarized recent advances regarding the molecular factors regulating seed size in Arabidopsis and other crops, followed by discussions on strategies to comprehend crops’ genetic and molecular aspects in balancing seed size and yield.

show abstract

“…In Oryza officinalis Wall. ex Watt, MKK3 encoding mitogen-activated protein kinase 3 is subject to alternative splicing and produces splicing variants, and overexpressing four of the five OsMKK3 splicing variants leads to reduced grain length and width ( Pan et al., 2021 ). Wheat has very huge and complex genome, and substantial wheat genes have complicated roles ( Singh et al., 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Two alternative splicing variants of a wheat gene TaNAK1, TaNAK1.1 and TaNAK1.2, differentially regulate flowering time and plant architecture leading to differences in seed yield of transgenic Arabidopsis

Zhang

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

In wheat production, appropriate flowering time and ideal plant architecture are the prerequisites for high grain yield. Alternative splicing (AS) is a vital process that regulates gene expression at the post-transcriptional level, and AS events in wheat have been found to be closely related to grain-related traits and abiotic stress tolerance. However, AS events and their biological roles in regulating flowering time and plant architecture in wheat remain unclear. In this study, we report that TaNAK1 undergoes AS, producing three splicing variants. Molecular characterization of TaNAK1 and its splicing variants demonstrated that all three protein isoforms have a conserved NB-ARC domain and a protein kinase domain, but the positions of these two domains and the length of the protein kinase domains are different among them, implying that they may have different three-dimensional structures and therefore have different functions. Further investigations showed that the two splicing variants of TaNAK1, TaNAK1.1 and TaNAK1.2, exhibited different expression patterns during wheat growth and development, while the other one, TaNAK1.3, was not detected. Subcellular localization demonstrated that TaNAK1.1 was mainly localized in the cytoplasm, while TaNAK1.2 was localized in the nucleus and cytoplasm. Both TaNAK1.1 and TaNAK1.2 exhibit protein kinase activity in vitro. Ectopic expression of TaNAK1.1 and TaNAK1.2 in Arabidopsis demonstrated that these two splicing variants play opposite roles in regulating flowering time and plant architecture, resulting in different seed yields. TaNAK1.2 positive regulates the transition from vegetative to reproductive growth, plant height, branching number, seed size, and seed yield of Arabidopsis, while TaNAK1.1 negatively regulates these traits. Our findings provide new gene resource for regulating flowering time and plant architecture in crop breeding for high grain yield.

show abstract

OrMKK3 Influences Morphology and Grain Size in Rice

Cited by 7 publications

References 71 publications

Natural Variation in OsMKK3 Contributes to Grain Size and Chalkiness in Rice

Natural Variation in OsMKK3 Contributes to Grain Size and Chalkiness in Rice

Developing Genetic Engineering Techniques for Control of Seed Size and Yield

Two alternative splicing variants of a wheat gene TaNAK1, TaNAK1.1 and TaNAK1.2, differentially regulate flowering time and plant architecture leading to differences in seed yield of transgenic Arabidopsis

Contact Info

Product

Resources

About