Ornamental flowers in new light: Artificial lighting shapes the microbial phyllosphere community structure of greenhouse grown sunflowers (Helianthus annuus L.)

Alsanius, Beatrix; Bergstrand, Karl-Johan; Hartmann, R.; Gharaie, Samareh; Wohanka, W.; Dorais, Martine; Rosberg, Anna Karin

doi:10.1016/j.scienta.2017.01.022

Cited by 40 publications

(42 citation statements)

References 40 publications

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“…The population counts measured on different microbiological laboratory media in terms of log CFU g −1 fresh weight ranged from 5 to 7, with the majority recording 6, except for R2A having 7. Our values corroborate with the data of other researchers documenting log CFU ml −1 in the range of 1-8 [23,24] in various plant species. However, the use of standard laboratory media provides bacterial isolates which represent only a small fraction of copiotrophs, even from the soil environments [25].…”

Section: Discussionsupporting

confidence: 93%

Influence of fertilizers and rice cultivation methods on the abundance and diversity of phyllosphere microbiome

et al. 2017

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Rice paddies are man-made, cross-over ecologies of aquatic and terrestrial systems, which favor the proliferation of characteristic microbial communities. Moisture regimes under flooded and different levels of irrigation such as in direct seeded rice (DSR) and system of rice intensification (SRI) lead to modulation in crop physiology, soil nutrient availability, and the soil microbiome. However, the diversity of the rice phyllosphere microbiome is less investigated in terms of the influence of fertilizer application and the method of rice cultivation (conventional-flooded, DSR and SRI). Scanning electron micrographs revealed the presence of bacteria as aggregates at microsites of the leaves. Phylogenetic analysis of the dominant culturable bacterial isolates using 16S rDNA sequences revealed that they belonged to the genera - Bacillus, Brevibacillus, Pantoea, Enterobacter, Pseudomonas, Erwinia, and Streptomyces. Fertilizer application brought about a distinct modulation in the communities belonging to phyla such as Bacteriodetes, Firmicutes, and Planctomyces, besides Proteobacteria. The cyanobacterial population was much influenced by the cultivation methods, particularly the SRI. Principal component analysis (PCA), involving the culturable phyllospheric microbial groups and leaf attributes (nutrients and pigments), illustrated the importance of leaf nitrogen and zinc. Also, the communities of the phylum Firmicutes exhibited marked changes in terms of the diversity, not only due to the cultivation method, but also the application of fertilizers. Thus, the cultivation methods and fertilizer application played important roles in modulating both the structural (taxonomical) and functional attributes of the phyllosphere microbiome.

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Section: Discussionsupporting

confidence: 93%

Influence of fertilizers and rice cultivation methods on the abundance and diversity of phyllosphere microbiome

et al. 2017

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“…Physiological changes in the plant caused by different light qualities have a major impact on the phyllosphere microbiota. In sunflower plants grown under HPS lamps, white LEDs, or red:blue (80:20 ratio) LEDs, it has been shown that the fungal communities are more affected than the bacterial communities by different light qualities [51]. Although that study did not investigate whether the effects on the microbiota are direct or indirect, other research (presented below) suggests that the effect is often caused by physiological alterations in the plant.…”

Section: Direct Plant-microbe Interactions Induced By Lightmentioning

confidence: 90%

“…With respect to foliar pathogens, the focus in previous research has been on alternative control of fungi using different wavelengths of light (light qualities), rather than on bacteria [46][47][48][49][50]. However, different light technologies influence biotic and abiotic conditions in the plant environment [51]. Modifications in the cropping environment, induced by light intensity and quality and by daylength, influence the structure but also the function of the leaf-associated microbiome [51][52][53].…”

Section: Introductionmentioning

confidence: 99%

“…However, different light technologies influence biotic and abiotic conditions in the plant environment [51]. Modifications in the cropping environment, induced by light intensity and quality and by daylength, influence the structure but also the function of the leaf-associated microbiome [51][52][53]. Microbes switch lifestyle to adapt to light qualities, as a matter of life and death (i.e., to enable metabolism, function, survival, growth, and nutrient acquisition) [50,52,53].…”

Section: Introductionmentioning

confidence: 99%

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Light and Microbial Lifestyle: The Impact of Light Quality on Plant–Microbe Interactions in Horticultural Production Systems—A Review

et al. 2019

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Horticultural greenhouse production in circumpolar regions (>60 • N latitude), but also at lower latitudes, is dependent on artificial assimilation lighting to improve plant performance and the profitability of ornamental crops, and to secure production of greenhouse vegetables and berries all year round. In order to reduce energy consumption and energy costs, alternative technologies for lighting have been introduced, including light-emitting diodes (LED). This technology is also well-established within urban farming, especially plant factories. Different light technologies influence biotic and abiotic conditions in the plant environment. This review focuses on the impact of light quality on plant-microbe interactions, especially non-phototrophic organisms. Bacterial and fungal pathogens, biocontrol agents, and the phyllobiome are considered. Relevant molecular mechanisms regulating light-quality-related processes in bacteria are described and knowledge gaps are discussed with reference to ecological theories.

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“…When a covering canopy has developed, this results in light quality stratification as basal leaves are shaded (Alsanius et al, 2017). While managing irradiation to mitigate enteric pathogens may not be feasible in a field setting, it may have some potential in a greenhouse setting where light conditions are more controlled.…”

Section: Abiotic Factors To Be Considered In the Hurdle Approachmentioning

confidence: 99%

The Hurdle Approach–A Holistic Concept for Controlling Food Safety Risks Associated With Pathogenic Bacterial Contamination of Leafy Green Vegetables. A Review

et al. 2018

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Consumers appreciate leafy green vegetables such as baby leaves for their convenience and wholesomeness and for adding a variety of tastes and colors to their plate. In Western cuisine, leafy green vegetables are usually eaten fresh and raw, with no step in the long chain from seed to consumption where potentially harmful microorganisms could be completely eliminated, e.g., through heating. A concerning trend in recent years is disease outbreaks caused by various leafy vegetable crops and one of the most important foodborne pathogens in this context is Shiga toxin-producing Escherichia coli (STEC). Other pathogens such as Salmonella, Shigella, Yersinia enterocolitica and Listeria monocytogenes should also be considered in disease risk analysis, as they have been implicated in outbreaks associated with leafy greens. These pathogens may enter the horticultural value network during primary production in field or greenhouse via irrigation, at harvest, during processing and distribution or in the home kitchen/restaurant. The hurdle approach involves combining several mitigating approaches, each of which is insufficient on its own, to control or even eliminate pathogens in food products. Since the food chain system for leafy green vegetables contains no absolute kill step for pathogens, use of hurdles at critical points could enable control of pathogens that pose a human health risk. Hurdles should be combined so as to decrease the risk due to pathogenic microbes and also to improve microbial stability, shelf-life, nutritional properties and sensory quality of leafy vegetables. The hurdle toolbox includes different options, such as physical, physiochemical and microbial hurdles. The goal for leafy green vegetables is multi-target preservation through intelligently applied hurdles. This review describes hurdles that could be used for leafy green vegetables and their biological basis, and identifies prospective hurdles that need attention in future research.

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Ornamental flowers in new light: Artificial lighting shapes the microbial phyllosphere community structure of greenhouse grown sunflowers (Helianthus annuus L.)

Cited by 40 publications

References 40 publications

Influence of fertilizers and rice cultivation methods on the abundance and diversity of phyllosphere microbiome

Influence of fertilizers and rice cultivation methods on the abundance and diversity of phyllosphere microbiome

Light and Microbial Lifestyle: The Impact of Light Quality on Plant–Microbe Interactions in Horticultural Production Systems—A Review

The Hurdle Approach–A Holistic Concept for Controlling Food Safety Risks Associated With Pathogenic Bacterial Contamination of Leafy Green Vegetables. A Review

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