Ornamental tobacco floral nectar is a rich source of antimicrobial peptides

Parra, Aura LC; Freitas, Cleverson D. T.; Souza, Pedro F.N.; Aderkas, Patrick von; Borchers, Christoph H.; Beattie, Gwyn A.; Silva, Fredy D.A.; Thornburg, Robert W.

doi:10.1016/j.plantsci.2022.111427

Cited by 5 publications

(5 citation statements)

References 61 publications

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“…Membranes are the main target of synthetic or natural peptides [ 16 , 24 ]. This is different from antibiotics, which generally affect a protein leading to the rapid development of resistance.…”

Section: Resultsmentioning

confidence: 99%

New Insights into the Mechanism of Antibacterial Action of Synthetic Peptide Mo-CBP3-PepI against Klebsiella pneumoniae

et al. 2022

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Klebsiella pneumoniae is a multidrug-resistant opportunistic human pathogen related to various infections. As such, synthetic peptides have emerged as potential alternative molecules. Mo-CBP3-PepI has presented great activity against K. pneumoniae by presenting an MIC50 at a very low concentration (31.25 µg mL−1). Here, fluorescence microscopy and proteomic analysis revealed the alteration in cell membrane permeability, ROS overproduction, and protein profile of K. pneumoniae cells treated with Mo-CBP3-PepI. Mo-CBP3-PepI led to ROS overaccumulation and membrane pore formation in K. pneumoniae cells. Furthermore, the proteomic analysis highlighted changes in essential metabolic pathways. For example, after treatment of K. pneumoniae cells with Mo-CBP3-PepI, a reduction in the abundance of protein related to DNA and protein metabolism, cytoskeleton and cell wall organization, redox metabolism, regulation factors, ribosomal proteins, and resistance to antibiotics was seen. The reduction in proteins involved in vital processes for cell life, such as DNA repair, cell wall turnover, and protein turnover, results in the accumulation of ROS, driving the cell to death. Our findings indicated that Mo-CBP3-PepI might have mechanisms of action against K. pneumoniae cells, mitigating the development of resistance and thus being a potent molecule to be employed in producing new drugs against K. pneumoniae infections.

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Section: Resultsmentioning

confidence: 99%

New Insights into the Mechanism of Antibacterial Action of Synthetic Peptide Mo-CBP3-PepI against Klebsiella pneumoniae

et al. 2022

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“…This peptide exhibited a significant inhibitory effect against Staphylococcus aureus , with a minimum inhibitory concentration (MIC) of 2 mg/mL. Parra, ALC et al (2022) [ 200 ] investigated AMPs from ornamental tobacco floral nectar, selecting six peptides for additional characterization, synthesis, and assessment of their antimicrobial efficacy against plant pathogenic fungi and bacteria. The results demonstrated that all six peptides exhibited certain antibacterial activity.…”

Section: Application Of Ms In Amps Studymentioning

confidence: 99%

Applications of tandem mass spectrometry (MS/MS) in antimicrobial peptides field: Current state and new applications

Fang,

Yu,

et al. 2024

Heliyon

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“…This conclusion resulted from using fluorophore-reactive propidium iodide (PI). Its presence in the tested configurations confirms that the membrane was degraded, with the specific mechanism not being elucidated [ 95 ].…”

Section: Mechanisms Of Actionmentioning

confidence: 99%

Membrane-Active Peptides and Their Potential Biomedical Application

Gostaviceanu,

Gavrilaş,

Copolovici

et al. 2023

Pharmaceutics

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Membrane-active peptides (MAPs) possess unique properties that make them valuable tools for studying membrane structure and function and promising candidates for therapeutic applications. This review paper provides an overview of the fundamental aspects of MAPs, focusing on their membrane interaction mechanisms and potential applications. MAPs exhibit various structural features, including amphipathic structures and specific amino acid residues, enabling selective interaction with multiple membranes. Their mechanisms of action involve disrupting lipid bilayers through different pathways, depending on peptide properties and membrane composition. The therapeutic potential of MAPs is significant. They have demonstrated antimicrobial activity against bacteria and fungi, making them promising alternatives to conventional antibiotics. MAPs can selectively target cancer cells and induce apoptosis, opening new avenues in cancer therapeutics. Additionally, MAPs serve as drug delivery vectors, facilitating the transport of therapeutic cargoes across cell membranes. They represent a fascinating class of biomolecules with significant potential in basic research and clinical applications. Understanding their mechanisms of action and designing peptides with enhanced selectivity and efficacy will further expand their utility in diverse fields. Exploring MAPs holds promise for developing novel therapeutic strategies against infections, cancer, and drug delivery challenges.

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Ornamental tobacco floral nectar is a rich source of antimicrobial peptides

Cited by 5 publications

References 61 publications

New Insights into the Mechanism of Antibacterial Action of Synthetic Peptide Mo-CBP3-PepI against Klebsiella pneumoniae

New Insights into the Mechanism of Antibacterial Action of Synthetic Peptide Mo-CBP3-PepI against Klebsiella pneumoniae

Applications of tandem mass spectrometry (MS/MS) in antimicrobial peptides field: Current state and new applications

Membrane-Active Peptides and Their Potential Biomedical Application

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