Orodispersible hyaluronic acid film delivery for oral wound healing in rats

Lee, Jeong Hyun; Lee, Ko Eun; Nam, Ok Hyung; Chae, Yong Kwon; Lee, Myoung-Han; Kweon, Dong-Keon; Kim, Mi Sun; Lee, Hyo-Seol; Choi, Sung Chul

doi:10.1016/j.jds.2022.04.004

Cited by 12 publications

(6 citation statements)

References 37 publications

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“…Similarly, Cho et al (2021) found that HA lms provided a faster healing rate in rat perianal wounds by increasing re-epithelialization and broblast proliferation. Moreover, this nding is supported by recent in vivo studies, which suggested that HA lms accelerate the healing of palatal and tongue biopsy wounds (Lee et al, 2024, Lee et al, 2022. Collectively, the available evidence supports the potential use of HA for oral wound healing.…”

Section: Introductionsupporting

confidence: 52%

Bilayer cellulose-coated hyaluronic acid-based scaffold for accelerating oral wound healing

Jung,

Ye,

Lee

et al. 2024

Preprint

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Objectives To assess the effects of hyaluronic acid (HA)-based scaffolds on oral wound healing. Materials and Methods A film-type 3% HA scaffold with bilayer cellulose coating was prepared and compared with one without coating. For cytocompatibility, human gingival fibroblasts were exposed to both scaffolds. Cell viability, flow cytometry, and scratch wound assays were performed. Additionally, in vivo and` ex vivo wound-healing assays were also conducted. Results Cytocompatibility tests showed no cytotoxicity for either HA scaffold. The scratch wound assay revealed a significant reduction in the open wound area in both HA scaffolds compared with the control (p < 0.05). However, no differences were observed in the open wound areas between the scaffolds with and without cellulose coating. Regarding in vivo wound healing analysis, the healing rates on day 3 were significantly higher in the HA scaffolds than in the control (p < 0.05), and there were no differences between the scaffolds. The HA scaffolds with the coating showed lower CD68 and higher vimentin expression than the control (p < 0.05). In contrast, the HA scaffolds without the coating did not. The ex vivo wound healing analysis showed significantly higher re-epithelialization rates in both scaffolds compared to the control (p < 0.05). Conclusions Within the limits of this study, the HA scaffold with coating showed enhanced wound-healing efficacy, indicating its potential for oral wound-healing applications.

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Section: Introductionsupporting

confidence: 52%

Bilayer cellulose-coated hyaluronic acid-based scaffold for accelerating oral wound healing

Jung,

Ye,

Lee

et al. 2024

Preprint

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“…Low molecular weight (0.8–1.2 MDa) HA films were prepared with a diameter of 7 mm according to previous studies (Kweon & Park, 2021; Lee, Lee, et al, 2022; Lee, Park, et al, 2022). The amount of HA was equivalent to 100 mL of 1% HA gel (Gengigel®; Ricerfarma).…”

Section: Methodsmentioning

confidence: 99%

Effect of orodispersible hyaluronic acid film on palatal mucosa wound healing

Lee

Kang

et al. 2023

Oral Diseases

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ObjectiveThe objective of the study was to evaluate the healing effect of hyaluronic acid films on palatal wounds.Materials and MethodsAfter making 5‐mm diameter palatal wounds, 72 rats were randomly assigned to three groups: control, hyaluronic acid gel, and hyaluronic acid film. The animals were sacrificed at 3, 7, and 21 days after the experiment. Clinical, histological, and RT‐PCR analyses were performed. Human ex vivo oral mucosa models were used. Histological analysis and pan‐cytokeratin staining were performed at 5 days after wound creation.ResultsIn rat model, both gels and films showed favorable healing on Days 3 and 7 compared with healing in the control (p < 0.05). Film showed remarkable VEGF and α‐SMA expression than did the others (p < 0.05). Immunohistochemical analysis showed that film exhibited significantly lower CD68 and greater α‐SMA and vimentin expression levels than those in the others (p < 0.05). In human model, re‐epithelialization rate of film group was significantly higher than that of the others. Complete epithelial regeneration was confirmed only in film group using pan‐cytokeratin staining.ConclusionsWithin the limits of this study, hyaluronic acid film outperformed gels in terms of palatal wound healing in both models.

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“…We have only quoted a paper reports the HA production by Klebsiellae sp. L-10 NTG 50 in a concentration of 2900 mg L − 1 and by K. pneumonia strain in concentration of 48 mg L − 1 [52]. Hence, K. pneumoniae H15 was nominated as a promising isolate for HA production in this study.…”

Section: Discussionmentioning

confidence: 95%

Hyaluronic acid production by Klebsiella pneumoniae strain H15 (OP354286) under different fermentation conditions

Ahmed,

Enan,

Saed

et al. 2023

Preprint

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Background Hyaluronic acid (HA) has gained significant attention due to its unique physical, chemical, and biological properties, making it widely used in various industries. This study aimed to screen bacterial isolates for HA production, characterize favorable fermentation conditions, and evaluate the inhibitory effect of bacterial HA on cancer cell lines. Results A total of 108 bacterial isolates from diverse sources were screened for HA production using HPLC, turbidity, and carbazole determination methods. Among the HA-producing isolates, Klebsiella pneumoniae H15 isolated from an animal feces sample, was superior in HA production. The strain was characterized based on morphological, cultural, and VITEK MS methods. Molecular identification using 16S rDNA sequencing and phylogenetic analysis confirmed its identity. Fermentation conditions, including pH, temperature, time, and agitation rate, were figured out to maximize HA production. The basal medium, comprising sucrose (7.0%) as the carbon source and yeast extract + peptone (1.25% each) as nitrogen substrates, favored the highest HA production at pH 8.0, for 30 h, at 30°C, and 180 rpm agitation. The average maximized HA concentration reached 1.5 g L− 1. Furthermore, bacterial HA exhibited a significant inhibitory effect on three cancer cell lines, with the lowest concentration ranging from 0.98–3.91 µg mL− 1. Conclusions K. pneumoniae H15 isolated from animal feces demonstrated promising potential for HA production. The most favorable fermentation conditions led to a high HA yield. The inhibitory effect of bacterial HA on cancer cell lines highlights its potential therapeutic applications. These findings contribute to the understanding and utilization of HA in various industries and therapeutic applications.

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Orodispersible hyaluronic acid film delivery for oral wound healing in rats

Cited by 12 publications

References 37 publications

Bilayer cellulose-coated hyaluronic acid-based scaffold for accelerating oral wound healing

Bilayer cellulose-coated hyaluronic acid-based scaffold for accelerating oral wound healing

Effect of orodispersible hyaluronic acid film on palatal mucosa wound healing

Hyaluronic acid production by Klebsiella pneumoniae strain H15 (OP354286) under different fermentation conditions

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